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AB300087

Anti-Tetanus toxoid antibody [35F7]

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Mouse Recombinant Monoclonal TETX antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Clostridium tetani, Clostridium tetani samples.

대체 명칭 보기

CTC_p60, tetX, Tetanus toxin, Tentoxylysin

1 이미지
Western blot - Anti-Tetanus toxoid antibody [35F7] (AB300087)
  • WB

Supplier Data

Western blot - Anti-Tetanus toxoid antibody [35F7] (AB300087)

This clone recognizes the epitope of tetanus toxoid heavy chain fragment C.Blocking and diluting buffer and concentration : 5% NFDM/TBST The 100 kDa band is expected to be the cleaved heavy chain . The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 10924793). Exposure time : 3 minutes

All lanes:

Western blot - Anti-Tetanus toxoid antibody [35F7] (ab300087) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containi a myc-His-tag®, whole cell lysate 10 µg

Lane 2:

HEK-293T transfected with Tetanus toxoid expression vector containi a myc-His-tag®, whole cell lysate 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 150 kDa,100 kDa

false

Exposure time: 3min

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-Tetanus toxoid antibody [35F7] - BSA and Azide free

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

35F7

Isotype

IgG1

Carrier free

No

Reacts with

Clostridium tetani

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB53829

Anti-Tetanus Toxin antibody

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Primary Antibodies

AB320652

Anti-hMPV fusion Protein antibody [EPR29365-579]

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Immunocytochemistry/ Immunofluorescence - Anti-hMPV fusion Protein antibody [EPR29365-579] (AB320652)

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Primary Antibodies

AB283946

Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174]

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Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (AB283946)

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Primary Antibodies

AB68494

Anti-Tetanus Toxoid antibody

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Clostridium tetani": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p>Use at 250 ng/ml</p>" }, "Transfected cell lysate - Clostridium tetani": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Tetanus Toxoid often referred to as TT is an inactivated toxin used in vaccines to prevent tetanus. It originates from the bacterium Clostridium tetani which produces the neurotoxin tetanospasmin. This toxoid has a mass of approximately 150 kDa and is conventionally administered as a vaccine in humans and animals. It triggers an immune response without causing disease as it's a detoxified version of the toxin. Tetanus toxoid is expressed in a laboratory setting where it undergoes chemical treatment to eliminate toxicity while maintaining its antigenicity.
Biological function summary

Tetanus toxoid stimulates the immune system to produce antibodies against the active tetanus toxin tetanospasmin. It doesn't form part of any specific protein complexes but functions independently to evoke humoral immunity. The binding of these antibodies to tetanospasmin neutralizes its effect thereby preventing the inhibition of neurotransmitter release. This process is important for avoiding the spasms and muscle rigidity associated with tetanus. The immune response it provokes includes the activation of B cells which differentiate into plasma cells that produce anti-tetanus antibodies.

Pathways

Tetanus toxoid plays an integral part in the adaptive immune response. It functions within the humoral immune pathway by stimulating the Th2 response. This activation is associated with B-cell proliferation and differentiation into antibody-secreting cells which provide long-term immunity. The vaccine-related immune response connects to key immunological proteins like CD4+ T-helper cells which play a central role in modulating the immune system's response to vaccines.

The tetanus toxoid vaccine is directly related to preventing tetanus an acute often fatal infection marked by severe muscle contractions. By inducing immunity tetanus toxoid plays a preventive role against tetanospasmin-mediated dysfunction in the nervous system. Additionally the concept of herd immunity associated with tetanus prevention helps in protecting community members including those unvaccinated. No other direct protein associations in disorders are typically highlighted but successful immunization inherently aids in broader public health by reducing incidences of infection.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-|-Phe-77' bond of synaptobrevin-2.
See full target information tetX
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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