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AB238140

Anti-Tetraspanin-33 antibody [EPR23121-146]

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Rabbit Recombinant Monoclonal Tetraspanin-33 antibody. Suitable for Flow Cyt, Dot and reacts with Human, Recombinant fragment - Human samples.

대체 명칭 보기

PEN, TSPAN33, Tetraspanin-33, Tspan-33, Penumbra, Proerythroblast new membrane, hPen

2 이미지
Flow Cytometry - Anti-Tetraspanin-33 antibody [EPR23121-146] (AB238140)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Tetraspanin-33 antibody [EPR23121-146] (AB238140)

Flow cytometric analysis of human peripheral blood mononuclear cells (PBMC) treated with 1ug/ml human CD40 ligand and 100U/ml human IL-4 for 72h labeling Tetraspanin-33 with ab238140 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG Isotype control (ab172730) (Left). Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG (Left) or ab238140 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.

Gated on viable cells.

Dot Blot - Anti-Tetraspanin-33 antibody [EPR23121-146] (AB238140)
  • Dot

Supplier Data

Dot Blot - Anti-Tetraspanin-33 antibody [EPR23121-146] (AB238140)

Dot blot analysis of Tetraspanin-33 labeled with ab238140 at 1/1000 dilution.

Lane 1 : Recombinant tetraspanin-33 fragment containing the sequence at the immunogen region.
Lane 2 : BSA.

Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-Tetraspanin-33 antibody [EPR23121-146] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23121-146

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB204950

Anti-hCG receptor/LHR antibody [8G9A2]

Flow Cytometry - Anti-hCG receptor/LHR antibody [8G9A2] (AB204950)

  • 4
  • 1
Sample Prep & Detection Kits

AB201807

APC Conjugation Kit - Lightning-Link®

Fluorescence Microscopy - APC Conjugation Kit - Lightning-Link® (AB201807)

  • 0
  • 0
Primary Antibodies

AB318916

Alexa Fluor® 555 Anti-CYP11A1 antibody [EPR24868-86]

RabMAb|Recombinant

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Primary Antibodies

AB284387

Anti-BMP2 antibody [EPR24209-61]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-BMP2 antibody [EPR24209-61] (AB284387)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Tetraspanin-33 also known as TSPAN33 or TSPN33 is a protein belonging to the tetraspanin family. It plays a role in organizing other proteins into functional complexes on the cell surface. Tetraspanin-33 weighs approximately 34 kDa and is mainly expressed in hematopoietic tissues which include the bone marrow and lymphoid organs. It is a transmembrane protein with four domains that spans the lipid bilayer influencing cell signaling by clustering with integrins and other membrane proteins.
Biological function summary

The organization of proteins into complexes forms an important function of Tetraspanin-33 in the immune system. It regulates cell adhesion migration and signaling impacting various types of immune responses. Tetraspanin-33 interacts with integrins forming tetraspanin-enriched microdomains. Such interactions stabilize the functional arrangements of signaling molecules ensuring precise communication and activation in immune cells.

Pathways

Tetraspanin-33 has influential roles in integrin-mediated signaling and immune response pathways. It modulates beta-1 integrin activity and works alongside proteins such as CD9 and CD81 which are also tetraspanins involved in the orchestration of cell adhesion and motility. The signaling modulated by Tetraspanin-33 plays key roles in mediating the cell-cell interactions needed for effective immune function providing a scaffold necessary for the activation and regulation of signaling cascades.

Dysfunctions involving Tetraspanin-33 appear in immune system disorders and certain types of cancer. Aberrant expression or mutations can relate to conditions such as leukemia where Tetraspanin-33 interacts with proteins like CD53 another tetraspanin involved in immune cell signaling. Additionally alterations in Tetraspanin-33 function might contribute to autoimmune disorders highlighting its importance in maintaining immune system balance and responding to pathogens properly.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Part of TspanC8 subgroup, composed of 6 members that interact with the transmembrane metalloprotease ADAM10. This interaction is required for ADAM10 exit from the endoplasmic reticulum and for enzymatic maturation and trafficking to the cell surface as well as substrate specificity. Different TspanC8/ADAM10 complexes have distinct substrates (PubMed : 26686862, PubMed : 30463011, PubMed : 37516108). Plays an important role in normal erythropoiesis (By similarity). It has a role in the differentiation of erythroid progenitors (By similarity). Negatively regulates ligand-induced Notch activity probably by regulating ADAM10 activity (PubMed : 26686862). Mediates docking of ADAM10 to zonula adherens by interacting with ADAM10 and, in a PDZD11-dependent manner, with the zonula adherens protein PLEKHA7 (PubMed : 30463011).
See full target information TSPAN33
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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