JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB34733

Texas Red ® Anti-GST antibody

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Anti-GST antibody conjugated to Texas Red® is a goat polyclonal antibody suitable for Western blot.
1 이미지
Western blot - Texas Red ® Anti-GST antibody (AB34733)
  • WB

Supplier Data

Western blot - Texas Red ® Anti-GST antibody (AB34733)

Blocking Buffer for Fluorescent Western Blotting for 30 minutes at room temperature.

All lanes:

Western blot - Texas Red ® Anti-GST antibody (ab34733) at 1/1000 dilution

All lanes:

GST at 0.05 µg

Predicted band size: 25 kDa

Observed band size: 25 kDa

false

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugation

Texas Red®

Excitation/Emission

Ex: 589nm, Em: 615nm

Carrier free

No

Applications

WB

applications

Specificity

<p>Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.</p>

style
left-column

대체품

Primary Antibodies

AB3416

HRP Anti-GST antibody

ELISA - HRP Anti-GST antibody (AB3416)

  • 5
  • 3
Primary Antibodies

AB9085

Anti-GST antibody

Lab Essentials

Western blot - Anti-GST antibody (AB9085)

  • 5
  • 2
Primary Antibodies

AB92

Anti-GST antibody [3G10/1B3]

Lab Essentials

Western blot - Anti-GST antibody [3G10/1B3] (AB92)

  • 5
  • 3
Primary Antibodies

AB111947

Anti-GST antibody [EPR4236]

20ul selling size|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GST antibody [EPR4236] (AB111947)

  • 0
  • 0
Primary Antibodies

AB235384

Anti-GST antibody [EPR4236] - BSA and Azide free

RabMAb|Recombinant

Immunoprecipitation - Anti-GST antibody [EPR4236] - BSA and Azide free (AB235384)

  • 0
  • 0

보완 제품

Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB1187

HRP Anti-6X His tag® antibody

Western blot - HRP Anti-6X His tag® antibody (AB1187)

  • 4
  • 12
Primary Antibodies

AB49763

HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2]

Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (AB49763)

  • 5
  • 8
Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

Texas Red™ Sulfonyl Chloride (Molecular Weight 625 daltons). Fluorochrome/Protein Ratio: 2.0 moles Texas Red™ per mole of Goat IgG. Absorption Wavelength: 596 nm; Emission Wavelength: 620 nm.

Conjugation Reference: J. Titus, P. Haugland, S. Sharrow, D. Segal J. Immunol. Methods 50; 193, 1982.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer.
보관 버퍼
Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.
Biological function summary

GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.

Pathways

GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.

GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
See full target information Glutathione S-transferase class-mu 26 kDa isozyme

대체 명칭 보기

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature methods 9:1192-4 PubMed23142872

2012

Systematic reconstruction of RNA functional motifs with high-throughput microfluidics.

Applications

Unspecified application

Species

Unspecified reactive species

Lance Martin,Matthias Meier,Shawn M Lyons,Rene V Sit,William F Marzluff,Stephen R Quake,Howard Y Chang
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com