Anti-TGF beta Receptor I (phospho S165) antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-TGF beta Receptor I (phospho S165) antibody (AB112095)

ab112095, at a 1/100 dilution, staining TGF beta Receptor I in HeLa cells by Immunofluorescence, in the absence (left) and presence (right) of the phosphopeptide.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta Receptor I (phospho S165) antibody (AB112095)

Immunohistochemistry analysis of paraffin-embedded human-liver tissue staining with ab112095 at 1/200 dilution incubated at 4°C overnight. Sodium citrate pH 6.0 was used for antibody retrieval at 98°C for 20min. Secondary antibody was used at 1/200 dilution at room temperature for 30min. Negative control was the secondary antibody only.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta Receptor I (phospho S165) antibody (AB112095)

Immunohistochemistry analysis of paraffin-embedded rat spleen tissue staining with ab112095 at 1/200 dilution incubated at 4°C overnight. Sodium citrate pH 6.0 was used for antibody retrieval at 98°C for 20min. Secondary antibody was used at 1/200 dilution at room temperature for 30min. Negative control was the secondary antibody only.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta Receptor I (phospho S165) antibody (AB112095)

Immunohistochemistry analysis of paraffin-embedded rat kidney tissue staining with ab112095 at 1/200 dilution incubated at 4°C overnight. Sodium citrate pH 6.0 was used for antibody retrieval at 98°C for 20min. Secondary antibody was used at 1/200 dilution at room temperature for 30min. Negative control was the secondary antibody only.

• WB

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Western blot - Anti-TGF beta Receptor I (phospho S165) antibody (AB112095)

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Western blot - Anti-TGF beta Receptor I (phospho S165) antibody (ab112095) at 1/1000 dilution

All lanes:

Mouse liver cells

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• WB

CiteAb

Western blot - Anti-TGF beta Receptor I (phospho S165) antibody (AB112095)

Western Blotting using Anti-TGF beta Receptor I (phospho S165) antibody, ab112095. Publication image from Wojciech, S. et al., 2018, Nat Commun, 29572483. Legend direct from paper.

GPR50 phosphorylate and activate TβRI independently of TβRII. a SNU638 cells were transfected with the indicated plasmids, stimulated for 1 h with TGFβ (2 ng/mL) and pretreated or not overnight with SB431542 at 10 µM. p-Smad3 and total Smad3 levels and expression of transfected plasmids were determined by western blot in cell lysates. b Co-immunoprecipitation of GRP50 and TβRI in lysates of SNU638 cells expressing HA-TβRI alone or together with GPR50δ4. Total lysates were used as expression control (c) SNU638 cells were transfected with the indicated plasmids, stimulated TGFβ (2 ng/mL, 1 h). Total TβRI was immunoprecipitated with anti-Flag antibody and immunoblotted for anti-TβRIp-S165. Below, the same blot was immunoblotted with anti-Flag to show the amount of TβRI precipitation in cell lysates. d Phospho-Smad3 and Smad2/3 levels were determined in lysates of SNU638 cells stimulated or not with TGFβ (2 ng/mL, 1 h) and expressing the indicated proteins (as verified by western blot). The myc-MT2 melatonin receptor and the myc-MT2-GPR50Cter served as negative controls. Representative results are shown for all the panels. Similar results were obtained in at least two additional experiments. See also Supplementary Fig. 4

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