AB180745

Anti-THEA antibody [EPR18792]

Name: Anti-THEA antibody [EPR18792]
Brand: Abcam Limited
SKU: AB180745
Rating: 5 (1 reviews)

RabMAb
Recombinant
각 태그의 뜻

(1 리뷰)

(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal THEA antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 2 publications.

대체 명칭 보기

BFIT, KIAA0707, THEA, ACOT11, Acyl-coenzyme A thioesterase 11, Acyl-CoA thioesterase 11, Acyl-CoA thioester hydrolase 11, Adipose-associated thioesterase, Brown fat-inducible thioesterase, Palmitoyl-coenzyme A thioesterase

4 이미지

Supplier Data

Western blot - Anti-THEA antibody [EPR18792] (AB180745)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 3 minutes; Lane 2/3 : 15 seconds.

All lanes:

Western blot - Anti-THEA antibody [EPR18792] (ab180745) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

Human fetal heart tissue lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/10000 dilution

Predicted band size: 68 kDa

Observed band size: 68 kDa

true

Supplier Data

Western blot - Anti-THEA antibody [EPR18792] (AB180745)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 3 minutes; Lane 2/3 : 15 seconds.

This antibody can recognize 2 isoforms in human, the predicted MW is 68 & 67 KDa.

All lanes:

Western blot - Anti-THEA antibody [EPR18792] (ab180745) at 1/2000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 2:

LNCaP (human prostate cancer cell line) whole cell lysate at 20 µg

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 68 kDa

Observed band size: 67 kDa,68 kDa

true

Supplier Data

Western blot - Anti-THEA antibody [EPR18792] (AB180745)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

The expression profile observed is consistent with what has been described in the literature (PMID : 22427358 and PMID : 11696000).

All lanes:

Western blot - Anti-THEA antibody [EPR18792] (ab180745) at 1/1000 dilution

Lane 1:

Mouse brown adipose lysate at 20 µg

Lane 2:

Mouse liver lysate at 20 µg

Lane 3:

Mouse kidney lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 68 kDa

Observed band size: 68 kDa

true

Supplier Data

Western blot - Anti-THEA antibody [EPR18792] (AB180745)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1/2 : 1 minute; Lane 3 : 10 seconds; Lane 4 : 15 seconds.

All lanes:

Western blot - Anti-THEA antibody [EPR18792] (ab180745) at 1/1000 dilution

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Mouse spleen lysate at 10 µg

Lane 3:

Mouse kidney lysate at 10 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 41 kDa,47 kDa,68 kDa

Observed band size: 68 kDa

true

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18792

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

THEA also known as Threonine E1A or THREA1 is an important protein involved in cellular processes. This target has a molecular mass of approximately 42 kDa. THEA is expressed in various tissues with high levels found particularly in the brain and liver. Through its mechanical role THEA contributes to cellular metabolism and signaling by participating in enzymatic reactions critical for cell function.

Biological function summary

THEA influences several aspects of cell growth and maintenance. It operates as part of an enzymatic complex that facilitates the phosphorylation of substrate molecules essential for many signaling pathways. This activity supports cellular energy regulation and can affect the cell cycle and synaptic function. Its impact on cellular metabolism highlights its importance in tissue homeostasis indicating a wide range of biological influences.

Pathways

THEA plays a significant role in metabolic and signaling pathways. It interacts closely with the AMPK signaling pathway which is critical for energy balance and homeostasis. In these processes THEA can influence related proteins such as LKB1 and PKA which further propagate signals to activate downstream pathways that maintain energy homeostasis and cellular response to metabolic stress.

THEA's activity links to metabolic syndromes and neurodegenerative diseases. Its altered function can contribute to conditions like diabetes where metabolic pathways become dysregulated. It also associates with Alzheimer's disease with stress on the AMPK pathway. In these diseases interactions with proteins like Tau and GSK3β can modulate disease progression highlighting the diverse roles of THEA in pathological states.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Has an acyl-CoA thioesterase activity with a preference for the long chain fatty acyl-CoA thioesters hexadecanoyl-CoA/palmitoyl-CoA and tetradecanoyl-CoA/myristoyl-CoA which are the main substrates in the mitochondrial beta-oxidation pathway.

See full target information ACOT11

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of medical sciences 20:1427-1447 PubMed37790851

2023

STARD12/14 are diagnostic and prognostic biomarkers of lung adenocarcinoma associated with epigenetic regulation, immune infiltration and ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Di Zhang,Dong-Mei Hu,Zhuang-E Shi,Qing-Xiang Wang,Meng-Yu Zhang,Jian-Yu Liu,Xiu-Li Ji,Yi-Qing Qu

Nature communications 11:5303 PubMed33082339

2020

AKAP5 complex facilitates purinergic modulation of vascular L-type Ca channel Ca1.2.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Paz Prada,Arsalan U Syed,Gopireddy R Reddy,Miguel Martín-Aragón Baudel,Víctor A Flores-Tamez,Kent C Sasse,Sean M Ward,Padmini Sirish,Nipavan Chiamvimonvat,Peter Bartels,Eamonn J Dickson,Johannes W Hell,John D Scott,Luis F Santana,Yang K Xiang,Manuel F Navedo,Madeline Nieves-Cintrón

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-THEA antibody [EPR18792]

Western blot - Anti-THEA antibody [EPR18792] (AB180745)

Western blot - Anti-THEA antibody [EPR18792] (AB180745)

Western blot - Anti-THEA antibody [EPR18792] (AB180745)

Western blot - Anti-THEA antibody [EPR18792] (AB180745)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise