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AB17785

Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted

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(3 제품이 사용된 논문 )

Mouse Monoclonal antibody. Suitable for IHC-P and reacts with Human, Rat, Mouse samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing Thomsen-Friedenreich Antigen protein.

대체 명칭 보기

CD176, TF antigen

1 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted (AB17785)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted (AB17785)

Human colon adenocarcinoma stained with ab17785 (prediluted)

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

SPM320

Isotype

IgM

Light chain 타입

kappa

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

Galß1-3GalNAc

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

특성 및 보관 정보

제형
Liquid
Purity
IgM fraction
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Thomsen-Friedenreich antigen (TF antigen) also known as CD176 is a cryptic carbohydrate antigen exposed on the surface of cells particularly after malignant transformation. It has a molecular mass that varies depending on the glycoprotein carrier. TF antigen often exists in an inactive form within normal epithelial and endothelial cells masked by sialic acid and becomes exposed in cancerous and certain pathological conditions. Its structural core consists of a galactose beta 1-3 N-acetylgalactosamine configuration.
Biological function summary

The TF antigen plays roles in cell adhesion signal transduction and as a marker of cancer progression. It is not part of a larger protein complex but serves a significant role by participating in cellular recognition and immune response evasion. Researchers identified it as a pan-carcinoma antigen often used as a target for cancer immunotherapy due to its expression in many tumor tissues such as breast and colorectal cancers.

Pathways

TF antigen is involved in processes related to cell adhesion and immune evasion. These interactions often include pathways like the PI3K/AKT signaling pathway which contribute to cell survival and proliferation and involve proteins such as integrins and lectins. The alteration in pathways due to TF expression changes helps cancer cells migrate and grow making it an important target for analyzing tumor behavior.

TF antigen closely associates with cancer specifically aiding in tumorigenesis and metastasis in breast and colorectal cancers. Its expression correlates with proteins like MUC1 and MUC2 which are mucins often overexpressed and modified in these cancers. The exposure of TF antigen on these mucins can be a marker for malignancy and the interaction further disrupts normal cell function contributing to disease progression.

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biochemistry and biophysics reports 5:313-327 PubMed28955838

2016

Mangiferin attenuates oxidative stress induced renal cell damage through activation of PI3K induced Akt and Nrf-2 mediated signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Sukanya Saha,Pritam Sadhukhan,Krishnendu Sinha,Namrata Agarwal,Parames C Sil

PloS one 9:e89026 PubMed24586486

2014

Amelioration of aspirin induced oxidative impairment and apoptotic cell death by a novel antioxidant protein molecule isolated from the herb Phyllanthus niruri.

Applications

Unspecified application

Species

Mouse

Sudip Bhattacharyya,Shatadal Ghosh,Parames C Sil

International journal of cancer 132:2107-17 PubMed23055136

2012

Multifunctional nanobeacon for imaging Thomsen-Friedenreich antigen-associated colorectal cancer.

Applications

Unspecified application

Species

Human

Hironori Kumagai,Wellington Pham,Makoto Kataoka,Ken-Ichiro Hiwatari,James McBride,Kevin J Wilson,Hiroyuki Tachikawa,Ryoji Kimura,Kunio Nakamura,Eric H Liu,John C Gore,Shinji Sakuma
제품이 사용된 논문 모두 보기

Product promise

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