AB236903

Anti-ThrRS antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal ThrRS antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TARS1 aa 1-250.

대체 명칭 보기

TARS, TARS1, Threonyl-tRNA synthetase, Threonyl-tRNA synthetase 1, ThrRS

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ThrRS antibody (AB236903)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ThrRS antibody (AB236903)

ThrRS was immunoprecipitated from 0.5 mg A549 (human lung carcinoma cell line) whole cell lysate with ab236903 at 1/200 dilution.

Lane 1 : Control rabbit IgG IP in A549 whole cell lysate.

Lane 2 : ab236903 IP in A549 whole cell lysate.

Lane 3 : A549 whole cell lysate 10 μg (Input).

For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ThrRS antibody (AB236903)

Paraffin-embedded human colon cancer tissue stained for ThrRS using ab236903 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ThrRS antibody (AB236903)

Paraffin-embedded human brain tissue stained for ThrRS using ab236903 at 1/100 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TARS1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P26639

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ThrRS also known as threonyl-tRNA synthetase is an enzyme that attaches the amino acid threonine to its corresponding tRNA (tRNA^Thr) an important step in the translation process during protein synthesis. ThrRS's molecular mass is approximately 80 kDa. It is expressed in various tissues across many organisms showing high conservation from bacteria to humans. This enzyme ensures accurate pairing of threonine with its tRNA which is critical for translating genetic information into functional proteins.

Biological function summary

Threonyl-tRNA synthetase plays a role in cellular function by ensuring proteins are synthesized accurately and efficiently. ThrRS is a standalone enzyme and does not form part of an enzyme complex. Its correct functioning is essential for maintaining cellular homeostasis and ensuring the fidelity of the genetic code. Incorrect aminoacylation by ThrRS can lead to translational errors which may manifest in malfunctioning proteins.

Pathways

The activity of threonyl-tRNA synthetase is integral to the protein synthesis pathway which is part of the larger biological process of gene expression. This pathway involves collaboration with other aminoacyl-tRNA synthetases and overlaps with translation mechanisms. ThrRS is related to proteins such as serine-tRNA synthetase and alanine-tRNA synthetase as they also participate in aminoacylation facilitating accurate protein synthesis by adding the right amino acids to tRNA molecules.

Problems associated with threonyl-tRNA synthetase can lead to conditions like Charcot-Marie-Tooth disease a disorder affecting peripheral nerves and certain types of cancer. Mutations in the ThrRS gene can alter its enzymatic activity or stability potentially disrupting the translation process. Moreover issues with ThrRS can interact with defective proteins like glycyl-tRNA synthetase further impacting cellular function and contributing to disease pathologies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction : threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr) (PubMed : 25824639, PubMed : 31374204). Also edits incorrectly charged tRNA(Thr) via its editing domain, at the post-transfer stage (By similarity).

See full target information TARS1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21948 PubMed34569098

2021

A non-translational role of threonyl-tRNA synthetase in regulating JNK signaling during myogenic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Dai,Adriana Reyes-Ordoñez,Jae-Sung You,Jie Chen

Journal of experimental & clinical cancer research : CR 40:92 PubMed33691743

2021

Downregulation of lncRNA ZNF582-AS1 due to DNA hypermethylation promotes clear cell renal cell carcinoma growth and metastasis by regulating the N(6)-methyladenosine modification of MT-RNR1.

Applications

Unspecified application

Species

Unspecified reactive species

Wuping Yang,Kenan Zhang,Lei Li,Yawei Xu,Kaifang Ma,Haibiao Xie,Jingcheng Zhou,Lin Cai,Yanqing Gong,Kan Gong

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ThrRS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ThrRS antibody (AB236903)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ThrRS antibody (AB236903)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ThrRS antibody (AB236903)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise