AB239509

Anti-Thymidine Kinase 1/TK1 antibody [C1]

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(2 제품이 사용된 논문 )

Mouse Monoclonal Thymidine Kinase 1/TK1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TK1.

대체 명칭 보기

TK1

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

Paraffin-embedded human uterine fibroids tissue stained for Thymidine Kinase 1/TK1 using ab239509 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Immunocytochemistry/ Immunofluorescence - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

MCF7 (human breast adenocarcinoma cell line) cells stained for Thymidine Kinase 1/TK1 (green) using ab239509 at 30 μg/ml in ICC/IF, followed by FITC staining.

Supplier Data

Western blot - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

All lanes:

Western blot - Anti-Thymidine Kinase 1/TK1 antibody [C1] (ab239509) at 6 µg/mL

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 25 kDa

false

Supplier Data

Western blot - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

All lanes:

Western blot - Anti-Thymidine Kinase 1/TK1 antibody [C1] (ab239509) at 5 µg/mL

All lanes:

Recombinant human Thymidine Kinase 1/TK1 protein

Predicted band size: 25 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG2b

Light chain 타입

kappa

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TK1.

P04183

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-30 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-30 µg/mL", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

Purification 관련 사항

Purified from TCS.

보관 버퍼

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Thymidine Kinase 1 (TK1) also known as Thymidine Kinase isoform A is an enzyme involved in the phosphorylation of thymidine into thymidine monophosphate an important component in DNA synthesis. TK1 has a molecular weight of approximately 25 kDa. It is predominantly expressed in proliferating cells with expression levels peaking during the S phase of the cell cycle. You often find TK1 in tissues with high cell division rates like bone marrow and lymphatic tissues.

Biological function summary

This enzyme is critical for DNA replication and repair by providing deoxythymidine triphosphate (dTTP) a deoxynucleoside triphosphate. TK1 operates as a monomer but can form a tetrameric complex in its active state which enhances its affinity for substrates. TK1 can process various substrates including thymidine and thymidine analogs such as 5-bromo-2'-deoxyuridine (BrdU) and EdU. These substrates are vital in molecular biology as they help in measuring DNA synthesis in cell proliferation studies.

Pathways

The function of TK1 is central to the salvage pathway of nucleotide synthesis. This pathway allows recycling of thymidine from degraded DNA which is particularly important for rapidly dividing cells that need to ensure a sufficient supply of nucleotides. TK1 acts alongside other enzymes such as thymidylate synthase and ribonucleotide reductase to maintain nucleotide balance within the cell ensuring proper DNA synthesis and cellular proliferation.

TK1 levels often increase in cancerous tissues due to their elevated cell proliferation rates. Serum TK1 is a biomarker for various cancers like leukemia and breast cancer. Studies show a correlation between high TK1 activity and tumor aggressiveness and progression. Additionally interactions of TK1 with the protein p53 a tumor suppressor show that defects in TK1 regulation can contribute to genomic instability highlighting its role in oncogenesis.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cell-cycle-regulated enzyme of importance in nucleotide metabolism (PubMed : 9575153). Catalyzes the first enzymatic step in the salvage pathway converting thymidine into thymidine monophosphate (PubMed : 22385435). Transcriptional regulation limits expression to the S phase of the cell cycle and transient expression coincides with the oscillation in the intracellular dTTP concentration (Probable). Also important for the activation of anticancer and antiviral nucleoside analog prodrugs such as 1-b-d-arabinofuranosylcytosine (AraC) and 3c-azido-3c-deoxythymidine (AZT) (PubMed : 22385435).

See full target information TK1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of translational medicine 23:61 PubMed39806464

2025

The microenvironment cell index is a novel indicator for the prognosis and therapeutic regimen selection of cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Xian-Yan Yang,Nian Chen,Qian Wen,Yu Zhou,Tao Zhang,Ji Zhou,Cheng-Hui Liang,Li-Ping Han,Xiao-Ya Wang,Qing-Mei Kang,Xiao-Xia Zheng,Xue-Jia Zhai,Hong-Ying Jiang,Tian-Hua Shen,Jin-Wei Xiao,Yu-Xin Zou,Yun Deng,Shuang Lin,Jiang-Jie Duan,Jun Wang,Shi-Cang Yu

Frontiers in molecular biosciences 9:848463 PubMed35480888

2022

Identification of Novel Immune Cell-Relevant Therapeutic Targets and Validation of Roles of TK1 in BMSCs of Systemic Lupus Erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Fangru Chen,Jian Meng,Wenjie Yan,Mengjiao Wang,Yunfei Jiang,Jintao Gao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thymidine Kinase 1/TK1 antibody [C1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

Immunocytochemistry/ Immunofluorescence - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

Western blot - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

Western blot - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise