AB240947

Anti-Thymidylate Synthase antibody [SP112] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal Thymidylate Synthase antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

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Flow Cytometry (Intracellular) - Anti-Thymidylate Synthase antibody [SP112] - BSA and Azide free (AB240947)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Thymidylate Synthase antibody [SP112] - BSA and Azide free (AB240947)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Thymidylate Synthase with purified ab111153 at 1/200 dilution (0.78μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^®488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab111153).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [SP112] - BSA and Azide free (AB240947)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [SP112] - BSA and Azide free (AB240947)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling Thymidylate Synthase with ab111153 at 1/100 dilution (1.55 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human colon carcinoma, with some nuclear staining, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab111153 for 30 mins at room temperature.

This image was generated using ab111153, the same clone, but with a different buffer formulation.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [SP112] - BSA and Azide free (AB240947)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling Thymidylate Synthase with ab111153 at 1/100 dilution (1.55 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human hepatocellular carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab111153 for 30 mins at room temperature.

This image was generated using ab111153, the same clone, but with a different buffer formulation.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP112

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }

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제품 세부 정보

ab240947 is the carrier-free version of ab111153.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

Purification 관련 사항

Purified from TCS by protein A/G.

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Thymidylate Synthase (TS) also known as TYMS or TSase is an important enzyme involved in the de novo synthesis of thymidylate a nucleotide necessary for DNA replication and repair. TS catalyzes the methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) using 510-methylenetetrahydrofolate as a cofactor. It has an approximate molecular weight of 36 kDa. The enzyme is expressed in the cytoplasm of proliferating cells predominantly in tissues with high cellular turnover like bone marrow and the intestinal lining.

Biological function summary

Thymidylate synthase plays a critical role in DNA synthesis and cell division. As a member of the folate cycle it indirectly influences the supply of thymidine for DNA synthesis affecting cell proliferation. The enzyme generally functions as a homodimer which is necessary for its activity. Increased expression of TS usually correlates with high rates of cell division making it an important factor in rapidly growing tissues and tumors.

Pathways

Several important biological networks involve thymidylate synthase especially the folate and pyrimidine metabolism pathways. These pathways are essential for the maintenance of nucleotide pools within the cell. TS is functionally related to proteins like dihydrofolate reductase (DHFR) which also play roles in folate metabolism and DNA synthesis. Collectively these proteins ensure the proper balance of nucleotide precursors for DNA replication and repair.

Thymidylate synthase is closely associated with cancer and certain autoimmunity conditions. Overexpression of TS is commonly observed in various cancers as tumor cells require increased nucleotide synthesis for rapid proliferation. This makes TS a target for several anticancer drugs like 5-fluorouracil which inhibits TS activity. Additionally aberrations in enzymes like dihydropyrimidine dehydrogenase (DPD) could affect TS function leading to chemotherapeutic agent sensitivity in cancer treatments.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the mitochondrial and nuclear de novo thymidylate biosynthesis pathway.

See full target information TYMS

대체 명칭 보기

TS, OK/SW-cl.29, TYMS, Thymidylate synthase, TSase

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal for immunotherapy of cancer 11: PubMed37734877

2023

Bispecific antibody CD73xEGFR more selectively inhibits the CD73/adenosine immune checkpoint on cancer cells and concurrently counteracts pro-oncogenic activities of CD73 and EGFR.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Maria Ploeg,Douwe Freerk Samplonius,Xiao Xiong,Xiurong Ke,Mark Alexander Johannes Martinus Hendriks,Isabel Britsch,Anne Paulien van Wijngaarden,Hao Zhang,Wijnand Helfrich

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thymidylate Synthase antibody [SP112] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Thymidylate Synthase antibody [SP112] - BSA and Azide free (AB240947)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [SP112] - BSA and Azide free (AB240947)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [SP112] - BSA and Azide free (AB240947)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise