AB53170

Anti-Thyroid Hormone Receptor beta antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal Thyroid Hormone Receptor beta antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human THRB.

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody (AB53170)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody (AB53170)

ICC/IF image of ab53170 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53170, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-Thyroid Hormone Receptor beta antibody (AB53170)

All lanes:

Western blot - Anti-Thyroid Hormone Receptor beta antibody (ab53170) at 1/500 dilution

Lane 1:

LoVo cell extracts minus immunising peptide

Lane 2:

LoVo cell extracts plus immunising peptide

Predicted band size: 52 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human THRB. The exact immunogen used to generate this antibody is proprietary information.

P10828

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Thyroid Hormone Receptor beta (THRB) also known as c-erbA-2 and NR1A2 is a nuclear hormone receptor and transcription factor weighing approximately 55 kDa. THRB binds thyroid hormones and plays a central role in moderating gene expression. Expression of this receptor occurs in various tissues with notable presence in the liver brain and heart. As a transcription factor it influences the transcription of specific target genes upon binding to thyroid hormones primarily thyroxine (T4) and triiodothyronine (T3).

Biological function summary

This receptor regulates critical metabolic processes and plays a role in development. THRB functions as part of a larger receptor complex partnering with retinoid X receptor (RXR) to form a heterodimer. This dimer binds to thyroid hormone response elements (TREs) on DNA modulating the expression of genes involved in metabolism thermogenesis and development of the central nervous system. The receptor's activity impacts many key physiological functions ensuring proper growth and metabolic balance.

Pathways

THRB engages significantly in the thyroid hormone signaling pathway and the lipid degradation pathway. It interacts with proteins like the aforementioned RXR in promoting transcriptional activities. In its regulatory capacity THRB helps facilitate lipid metabolism cholesterol homeostasis and energy management. As part of the thyroid hormone signaling pathway it controls numerous genes aligning with proteins like thyroid peroxidase (TPO) which in turn affect hormone synthesis.

Mutations or malfunctions in THRB relate to conditions such as resistance to thyroid hormone (RTH) and medullary thyroid carcinoma. In RTH inappropriate THRB activity results in altered thyroid hormone action and impaired feedback mechanism. This connects to disrupted signaling involving proteins like thyroglobulin. In medullary thyroid carcinoma THRB alterations can influence tumor progression demonstrating its impact on cellular regulatory mechanisms. Understanding THRB's involvement in these disorders aids in diagnosing and developing targeted therapeutic approaches.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.

See full target information THRB

대체 명칭 보기

ERBA2, NR1A2, THR1, THRB, Thyroid hormone receptor beta, Nuclear receptor subfamily 1 group A member 2, c-erbA-2, c-erbA-beta

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Endocrinology 157:3278-92 PubMed27253998

2016

Thyroid Hormone Receptor-β (TRβ) Mediates Runt-Related Transcription Factor 2 (Runx2) Expression in Thyroid Cancer Cells: A Novel Signaling Pathway in Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Frances E Carr,Phillip W L Tai,Michael S Barnum,Noelle E Gillis,Katherine G Evans,Thomas H Taber,Jeffrey H White,Jennifer A Tomczak,Diane M Jaworski,Sayyed K Zaidi,Jane B Lian,Janet L Stein,Gary S Stein

The American journal of pathology 184:230-47 PubMed24215914

2013

The thyroid hormone triiodothyronine controls macrophage maturation and functions: protective role during inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Cristiana Perrotta,Marcella Buldorini,Emma Assi,Denise Cazzato,Clara De Palma,Emilio Clementi,Davide Cervia

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thyroid Hormone Receptor beta antibody

Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody (AB53170)

Western blot - Anti-Thyroid Hormone Receptor beta antibody (AB53170)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise