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AB27851

Anti-TIE1 antibody [8C9]

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(6 제품이 사용된 논문 )

Mouse Monoclonal TIE1 antibody. Carrier free. Suitable for Flow Cyt, WB and reacts with Human, Recombinant fragment samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TIE1.

대체 명칭 보기

TIE, TIE1, Tyrosine-protein kinase receptor Tie-1

1 이미지
Flow Cytometry - Anti-TIE1 antibody [8C9] (AB27851)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-TIE1 antibody [8C9] (AB27851)

Overlay histogram showing K562 cells stained with ab27851 (red line). The cells were fixed with 80% methanol (5 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27851, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

8C9

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human TIE1. The exact immunogen used to generate this antibody is proprietary information.

P35590

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대체품

Primary Antibodies

AB111547

Anti-TIE1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIE1 antibody (AB111547)

  • 0
  • 0
Primary Antibodies

AB201977

Anti-TIE1 antibody [8D12B10]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIE1 antibody [8D12B10] (AB201977)

  • 0
  • 0
Primary Antibodies

AB201986

Anti-TIE1 antibody [8D12D2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIE1 antibody [8D12D2] (AB201986)

  • 0
  • 0
Primary Antibodies

AB203078

Anti-TIE1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIE1 antibody (AB203078)

  • 0
  • 1
Primary Antibodies

AB236080

Anti-TIE1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIE1 antibody (AB236080)

  • 0
  • 0

보완 제품

Assay Kits

AB100649

Human TIE1 ELISA Kit

Sandwich ELISA - Human TIE1 ELISA Kit (AB100649)

  • 0
  • 0
Primary Antibodies

AB308524

Anti-ATP7A antibody [EPR27493-139]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-ATP7A antibody [EPR27493-139] (AB308524)

  • 0
  • 0
Primary Antibodies

AB227036

Anti-CCR6 antibody [EPR22259]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] (AB227036)

  • 0
  • 0
Primary Antibodies

AB182032

Anti-CD14 antibody [4B4F12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [4B4F12] (AB182032)

  • 5
  • 2
Primary Antibodies

AB315881

Anti-CLC3 antibody [EPR26497-64]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-CLC3 antibody [EPR26497-64] (AB315881)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "200000-500000 µg/mL", "WB-species-notes": "<p></p>" }, "Recombinant fragment": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "200000-500000 µg/mL", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
This antibody was purified from cell culture supernatant by chromatography.
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TIE1 also known as Tyrosine Kinase with Immunoglobulin-like and EGF-like Domains 1 plays a role as a receptor tyrosine kinase. It has a molecular mass of approximately 130 kDa. TIE1 is mainly expressed in vascular endothelial cells and is involved in the vascular system. It shares structural characteristics with other tyrosine kinases providing a framework for signaling and interaction. TIE1 activation involves phosphorylation leading to subsequent signaling cascades in endothelial cells.
Biological function summary

TIE1 associates with angiogenesis and blood vessel integrity. It forms a complex with other proteins notably TIE2 within the endothelial cells where it modulates endothelial cell survival and proliferation. Its role becomes particularly significant in dynamic environments where new blood vessel formation or remodeling occurs. TIE1 interacts with angiopoietins providing a network essential for maintaining vascular homeostasis and integrity.

Pathways

TIE1 interacts within angiopoietin-TIE signaling pathways. This pathway plays a critical role in vascular maturation and stability. TIE1 working in conjunction with TIE2 and the angiopoietins orchestrates the intricate balance between vessel stabilization and sprouting needed for healthy vascular function. These interactions link TIE1 with core processes in endothelial cell communication and vascular development.

TIE1 shows association with cancer and atherosclerosis. Its interaction with TIE2 and angiopoietin proteins contributes to pathological angiogenesis often observed in tumor growth and progression. In atherosclerosis TIE1 influences endothelial dysfunction and vascular inflammation. These diseases highlight TIE1's contribution to pathological processes highlighting the importance of its precise regulation in maintaining vascular health.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
See full target information TIE1
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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of cardiovascular and thoracic research 13:131-140 PubMed34326967

2021

Resveratrol reduced the detrimental effects of malondialdehyde on human endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mehdi Hassanpour,Çıgır Biray Avci,Reza Rahbarghazi,Aysa Rezabakhsh,Alireza Nourazarian,Elahe Nabat,Farzaneh Fathi,Majid Khaksar

Annals of translational medicine 9:232 PubMed33708859

2021

MiR-4729 regulates TIE1 mRNA m6A modification and angiogenesis in hemorrhoids by targeting METTL14.

Applications

Unspecified application

Species

Unspecified reactive species

Te Liu,Haikun Zhou,Hong Lu,Chunsheng Luo,Qingming Wang,Yunhua Peng,Wei Yang,Yaojie Xin

Frontiers in cell and developmental biology 9:629274 PubMed33644068

2021

Melatonin Promotes Heterotopic Ossification Through Regulation of Endothelial-Mesenchymal Transition in Injured Achilles Tendons in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhang,Jiajun Tang,Jie Liu,Bo Yan,Bin Yan,Minjun Huang,Zhongmin Zhang,Liang Wang

Stem cell research & therapy 11:139 PubMed32216836

2020

Autophagy modulation altered differentiation capacity of CD146 cells toward endothelial cells, pericytes, and cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Mehdi Hassanpour,Jafar Rezaie,Masoud Darabi,Amirataollah Hiradfar,Reza Rahbarghazi,Mohammad Nouri

Journal of cellular and molecular medicine 23:2595-2609 PubMed30672120

2019

Neurotrophin-3 acts on the endothelial-mesenchymal transition of heterotopic ossification in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhang,Liang Wang,He Cao,Nan Chen,Bin Yan,Xiang Ao,Huiyu Zhao,Jun Chu,Minjun Huang,Zhongmin Zhang

Nature communications 9:994 PubMed29520052

2018

Expansion of functional personalized cells with specific transgene combinations.

Applications

Unspecified application

Species

Unspecified reactive species

Christoph Lipps,Franziska Klein,Tom Wahlicht,Virginia Seiffert,Milada Butueva,Jeannette Zauers,Theresa Truschel,Martin Luckner,Mario Köster,Roderick MacLeod,Jörn Pezoldt,Jochen Hühn,Qinggong Yuan,Peter Paul Müller,Henning Kempf,Robert Zweigerdt,Oliver Dittrich-Breiholz,Thomas Pufe,Rainer Beckmann,Wolf Drescher,Jose Riancho,Carolina Sañudo,Thomas Korff,Bertram Opalka,Vera Rebmann,Joachim R Göthert,Paula M Alves,Michael Ott,Roland Schucht,Hansjörg Hauser,Dagmar Wirth,Tobias May
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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