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AB37910

Anti-TIGAR antibody

4

(3 리뷰들)

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(28 제품이 사용된 논문 )

Rabbit Polyclonal TIGAR antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 28 publications.
2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-TIGAR antibody (AB37910)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TIGAR antibody (AB37910)

ICC/IF image of ab37910 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37910 at 1μg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.

Western blot - Anti-TIGAR antibody (AB37910)
  • WB

Project

Western blot - Anti-TIGAR antibody (AB37910)

All lanes:

Western blot - Anti-TIGAR antibody (ab37910) at 1/250 dilution

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Western blot - Jurkat whole cell lysate (<a href='/ko/products/cell-lysates/jurkat-whole-cell-lysate-ab7899'>ab7899</a>) at 10 µg

Lane 3:

Western blot - A-431 whole cell lysate (<a href='/ko/products/cell-lysates/a-431-whole-cell-lysate-ab7909'>ab7909</a>) at 10 µg

Lane 4:

Western blot - HEK-293 whole cell lysate (<a href='/ko/products/cell-lysates/hek-293-whole-cell-lysate-ab7902'>ab7902</a>) at 10 µg

Lane 5:

Hep G2 whole cell lysate (<a href='/ko/products/unavailable/hep-g2-whole-cell-lysate-ab7900'>ab7900</a>) at 10 µg

Lane 6:

MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 30 kDa

Observed band size: 30 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB137573

Anti-TIGAR antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGAR antibody - C-terminal (AB137573)

  • 0
  • 0
Primary Antibodies

AB62533

Anti-TIGAR antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGAR antibody (AB62533)

  • 0
  • 0
Primary Antibodies

AB189164

Anti-TIGAR antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGAR antibody (AB189164)

  • 0
  • 0

보완 제품

Assay Kits

AB136955

Glucose Uptake Assay Kit (Colorimetric)

Functional Studies - Glucose Uptake Assay Kit (Colorimetric) (AB136955)

  • 5
  • 1
Primary Antibodies

AB109520

Anti-p21 antibody [EPR362]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-p21 antibody [EPR362] (AB109520)

  • 5
  • 17
Primary Antibodies

AB209847

Anti-Hexokinase II antibody [EPR20839]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-Hexokinase II antibody [EPR20839] (AB209847)

  • 5
  • 2
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
Primary Antibodies

AB181125

Anti-STING antibody [EPR13130]

20ul selling size|KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-STING antibody [EPR13130] (AB181125)

  • 4
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TIGAR also known as TP53-Induced Glycolysis and Apoptosis Regulator is a protein involved in cellular metabolism. With a mass of approximately 28 kDa TIGAR regulates glycolysis by acting similarly to fructose-26-bisphosphatase. It is expressed predominantly in tissues with active cellular proliferation such as the brain and liver. TIGAR functions mechanically to inhibit the glycolytic flux by decreasing levels of fructose-26-bisphosphate which is important for various cellular activities.
Biological function summary

TIGAR plays a critical role in controlling the oxidative stress within cells. By lowering glycolytic rates it shifts cellular metabolism toward the pentose phosphate pathway boosting the production of NADPH and reducing reactive oxygen species (ROS). This protein does not form a part of a larger complex operating independently to exhibit its effects. It also influences cellular survival mechanisms by mitigating oxidative stress which is important for cell health.

Pathways

TIGAR is involved with both apoptosis and carbohydrate metabolism pathways where it acts to support cellular metabolism and survival. Within these pathways it interacts closely with the tumor suppressor protein p53 which regulates TIGAR expression. Another related protein is AMPK which also plays roles in energy balance and is influenced by changes in cellular metabolic status. TIGAR's actions help in cellular adaptation during metabolic stress maintaining energy homeostasis.

TIGAR is linked to cancer and neurodegenerative diseases. Its role in reducing oxidative stress makes it significant in cancer as elevated levels can sometimes promote cancer cell survival. Involvement with p53 is particularly notable as alterations in p53 pathways are common in various cancers. In neurodegenerative diseases such as Alzheimer's TIGAR's function in managing oxidative stress can affect neuronal survival. Dysregulation of related metabolic processes can contribute to disease progression highlighting the importance of TIGAR in these contexts.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate (PubMed : 19015259). Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent manner, resulting in the pentose phosphate pathway (PPP) activation and NADPH production (PubMed : 16839880, PubMed : 22887998). Contributes to the generation of reduced glutathione to cause a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death (PubMed : 16839880, PubMed : 19713938, PubMed : 22887998, PubMed : 23726973, PubMed : 23817040). Plays a role in promoting protection against cell death during hypoxia by decreasing mitochondria ROS levels in a HK2-dependent manner through a mechanism that is independent of its fructose-bisphosphatase activity (PubMed : 23185017). In response to cardiac damage stress, mediates p53-induced inhibition of myocyte mitophagy through ROS levels reduction and the subsequent inactivation of BNIP3. Reduced mitophagy results in an enhanced apoptotic myocyte cell death, and exacerbates cardiac damage (By similarity). Plays a role in adult intestinal regeneration; contributes to the growth, proliferation and survival of intestinal crypts following tissue ablation (PubMed : 23726973). Plays a neuroprotective role against ischemic brain damage by enhancing PPP flux and preserving mitochondria functions (By similarity). Protects glioma cells from hypoxia- and ROS-induced cell death by inhibiting glycolysis and activating mitochondrial energy metabolism and oxygen consumption in a TKTL1-dependent and p53/TP53-independent manner (PubMed : 22887998). Plays a role in cancer cell survival by promoting DNA repair through activating PPP flux in a CDK5-ATM-dependent signaling pathway during hypoxia and/or genome stress-induced DNA damage responses (PubMed : 25928429). Involved in intestinal tumor progression (PubMed : 23726973).
See full target information TIGAR

대체 명칭 보기

C12orf5, TIGAR, TP53-induced glycolysis and apoptosis regulator, TP53-induced glycolysis regulatory phosphatase
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제품이 사용된 논문 (28)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in immunology 14:1114620 PubMed37122710

2023

TIGAR deficiency induces caspase-1-dependent trophoblasts pyroptosis through NLRP3-ASC inflammasome.

Applications

Unspecified application

Species

Unspecified reactive species

Junjun Guo,Meijuan Zhou,Man Zhao,Shuxian Li,Zhenya Fang,Anna Li,Meihua Zhang

Acta pharmaceutica Sinica. B 12:1871-1884 PubMed35847493

2022

Nuclear TIGAR mediates an epigenetic and metabolic autoregulatory loop NRF2 in cancer therapeutic resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Wang,Qianqian Wang,Guodi Cai,Zhijian Duan,Zoann Nugent,Jie Huang,Jianwei Zheng,Alexander D Borowsky,Jian Jian Li,Peiqing Liu,Hsing-Jien Kung,Leigh Murphy,Hong-Wu Chen,Junjian Wang

Nature metabolism 4:389-403 PubMed35347316

2022

Switching to the cyclic pentose phosphate pathway powers the oxidative burst in activated neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Emily C Britt,Jorgo Lika,Morgan A Giese,Taylor J Schoen,Gretchen L Seim,Zhengping Huang,Pui Y Lee,Anna Huttenlocher,Jing Fan

Cells 9: PubMed32823793

2020

A Scaffold-Free 3-D Co-Culture Mimics the Major Features of the Reverse Warburg Effect In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Florian Keller,Roman Bruch,Richard Schneider,Julia Meier-Hubberten,Mathias Hafner,Rüdiger Rudolf

Oncology letters 19:1322-1330 PubMed31966064

2020

IDH1-R132H mutation radiosensitizes U87MG glioma cells via epigenetic downregulation of TIGAR.

Applications

Unspecified application

Species

Unspecified reactive species

Narui Yin,Ting Xie,Haowen Zhang,Jian Chen,Jiahua Yu,Fenju Liu

Frontiers in oncology 9:1258 PubMed31799200

2019

TIGAR Promotes Tumorigenesis and Protects Tumor Cells From Oxidative and Metabolic Stresses in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhua Liu,Yue Wu,Yingqiu Zhang,Menglang Yuan,Xuelu Li,Jiyue Gao,Shanni Zhang,Chengjuan Xing,Huamin Qin,Hongbo Zhao,Zuowei Zhao

Molecular cancer research : MCR 17:1893-1909 PubMed31239287

2019

Cigarette Smoke Induces Metabolic Reprogramming of the Tumor Stroma in Head and Neck Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Domingo-Vidal,Diana Whitaker-Menezes,Cristina Martos-Rus,Patrick Tassone,Christopher M Snyder,Madalina Tuluc,Nancy Philp,Joseph Curry,Ubaldo Martinez-Outschoorn

International journal of molecular sciences 20: PubMed30823646

2019

Knockdown of the TP53-Induced Glycolysis and Apoptosis Regulator (TIGAR) Sensitizes Glioma Cells to Hypoxia, Irradiation and Temozolomide.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriele D Maurer,Sonja Heller,Christina Wanka,Johannes Rieger,Joachim P Steinbach

Cell death & disease 10:198 PubMed30814486

2019

TIGAR promotes neural stem cell differentiation through acetyl-CoA-mediated histone acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjuan Zhou,Tiantian Zhao,Jingyi Du,Guangyu Ji,Xinyue Li,Shufang Ji,Wenyu Tian,Xu Wang,Aijun Hao

Cancer management and research 10:5605-5616 PubMed30519107

2018

Prognostic value of TIGAR and LC3B protein expression in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wei,Jinxia Peng,Peng Wu,Ping Chen,Hongru Yang,Yongxia Cui,Linglin Yang
제품이 사용된 논문 모두 보기
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Product promise

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