AB288419

Anti-TIGIT antibody [1B4]

Recombinant
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Rabbit Recombinant Monoclonal TIGIT antibody. Suitable for ELISA, WB and reacts with Recombinant fragment, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Tigit.

대체 명칭 보기

Vstm3, Tigit, T-cell immunoreceptor with Ig and ITIM domains, V-set and transmembrane domain-containing protein 3

2 이미지

ELISA - Anti-TIGIT antibody [1B4] (AB288419)

ELISA

Supplier Data

ELISA - Anti-TIGIT antibody [1B4] (AB288419)

Binding curve of ab288419 to recombinant mouse TIGIT Fc-Fusion Protein. ELISA Plate coated with recombinant mouse TIGIT Fc-Fusion Protein at a concentration of 2 μg/ml. A 4-fold serial dilution from 5,000 ng/ml was performed using ab288419. For detection, a 1 : 4000 dilution of HRP-labelled anti-rabbit antibody was used.

Supplier Data

Western blot - Anti-TIGIT antibody [1B4] (AB288419)

ab288419 stained a band different to the expected MW as TIGIT is glycosylated which can make proteins run higher.

All lanes:

Western blot - Anti-TIGIT antibody [1B4] (ab288419) at 0.3 µg/mL

Lane 1:

RAW-264.7 cells (in RIPA buffer) at 35 µg

Lane 2:

Mouse spleen lysate (in RIPA buffer) at 35 µg

Lane 3:

Mouse thymus lysate (in RIPA buffer) at 35 µg

Secondary

All lanes:

Anti-rabbit antibody

Predicted band size: 26 kDa

false

Exposure time: 1hr

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

1B4

Isotype

IgG

Light chain 타입

lambda

Carrier free

Reacts with

Mouse

Applications

ELISA, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Tigit. The exact immunogen used to generate this antibody is proprietary information.

P86176

Specificity

This antibody is specific for murine TIGIT.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3 µg/mL", "WB-species-notes": "<p></p>" }, "Recombinant fragment": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.02% Proclin 300 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TIGIT also known as T cell immunoreceptor with Ig and ITIM domains is an immunoglobulin superfamily receptor. It weighs approximately 26 kDa. TIGIT commonly expresses on T cells especially on regulatory T cells (Tregs) and effector memory T cells. It also presents on natural killer (NK) cells. Among its alternate forms some notable ones also include the anti-TIGIT and biotinylated TIGIT antibodies used in experimental research to monitor immune responses.

Biological function summary

TIGIT acts as an inhibitory receptor modulating immune responses. It belongs to the CD28 family and binds to poliovirus receptor (PVR) and nectin-2 on antigen-presenting cells. This interaction inhibits T cell activation and proliferation and increases Treg cell activity maintaining immune homeostasis. TIGIT forms a complex with other inhibitory receptors like PD-1 2B4 and CD96 to further regulate immune functions.

Pathways

The interaction of TIGIT with its ligands significantly affects the immune checkpoint pathway. This involvement regulates T cell and NK cell activity. TIGIT is related to CD226 (DNAM-1) which competes for binding to its ligands influencing cellular signaling pathways. Through these pathways TIGIT signals through the downstream SHP-1 and SHP-2 proteins affecting immune cell signaling and response.

TIGIT has implications in cancer and autoimmune diseases. Its inhibitory function can allow tumor progression by dampening effective anti-tumor immunity. In cancer TIGIT’s activity often pairs with PD-1 both contributing to immune checkpoint blockade resistance necessary for tumor evasion. In autoimmune diseases increased TIGIT expression could result in reduced immune cell attack against self tissues showing its potential to modulate treatment strategies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades. In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway. Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity.

See full target information Tigit

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

World journal of surgical oncology 21:61 PubMed36823639

2023

Identification of critical prognosis signature associated with lymph node metastasis of stomach adenocarcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Wang,Wei Zhang,Yulin Guo,Yifei Zhang,Xiaofeng Bai,Yibin Xie

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIGIT antibody [1B4]

ELISA - Anti-TIGIT antibody [1B4] (AB288419)

Western blot - Anti-TIGIT antibody [1B4] (AB288419)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise