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AB324717

Anti-TIGIT antibody [EPR28147-69]

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Rabbit Recombinant Monoclonal TIGIT antibody. Suitable for Flow Cyt and reacts with Mouse samples.
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Flow Cytometry - Anti-TIGIT antibody [EPR28147-69] (AB324717)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TIGIT antibody [EPR28147-69] (AB324717)

Flow cytometric analysis of Mouse spleen cells were treated with 5ug/ml of immobilized anti-CD3 and anti-CD28 for 24 hours (Lower left and right) / Untreated control (Upper left and right) cells labelling TIGIT with ab324717 at 1/500 dilution (0.1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells were co-stained with CD3 conjugated to Alexa Fluor® 647.

Flow Cytometry - Anti-TIGIT antibody [EPR28147-69] (AB324717)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TIGIT antibody [EPR28147-69] (AB324717)

Flow cytometric analysis of Mouse spleen cells were treated with 5ug/ml of immobilized anti-CD3 and anti-CD28 for 24 hours (Lower left and right) / Untreated control (Upper left and right) cells labelling TIGIT with ab324717 at 1/500 dilution (0.1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells were co-stained with CD25 conjugated to Brilliant Violet 421.

Flow Cytometry - Anti-TIGIT antibody [EPR28147-69] (AB324717)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TIGIT antibody [EPR28147-69] (AB324717)

Flow cytometric analysis of Mouse spleen cells were treated with 5ug/ml of immobilized anti-CD3 and anti-CD28 for 24 hours (Lower left and right) / Untreated control (Upper left and right) cells labelling TIGIT with ab324717 at 1/500 dilution (0.1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells were co-stained with CD19 conjugated to PE/Cy7.

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-TIGIT antibody [EPR28147-69] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28147-69

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

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Primary Antibodies

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Primary Antibodies

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  • 4
  • 5
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades. In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway. Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity.
See full target information Tigit

대체 명칭 보기

Vstm3, Tigit, T-cell immunoreceptor with Ig and ITIM domains, V-set and transmembrane domain-containing protein 3
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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