AB167423

Anti-TIM22 antibody [EPR9973]

Name: Anti-TIM22 antibody [EPR9973]
Brand: Abcam Limited
SKU: AB167423
Rating: 4 (1 reviews)

RabMAb
Recombinant
각 태그의 뜻

(1 리뷰)

(5 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal TIM22 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.

대체 명칭 보기

TEX4, TIM22, TIMM22, Mitochondrial import inner membrane translocase subunit Tim22, Testis-expressed protein 4

1 이미지

Unknown

Western blot - Anti-TIM22 antibody [EPR9973] (AB167423)

All lanes:

Western blot - Anti-TIM22 antibody [EPR9973] (ab167423) at 1/1000 dilution

Lane 1:

A673 cell lysate at 10 µg

Lane 2:

T47D cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

BxPC-3 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP antibody at 1/2000 dilution

Predicted band size: 20 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9973

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The TIM22 protein also known as Translocase of Inner Mitochondrial Membrane 22 has a molecular mass of approximately 20 kilodaltons. It plays an important mechanical role in the import and insertion of membrane proteins into the mitochondrial inner membrane. TIM22 locates itself in the mitochondrial inner membrane where it functions as part of a larger translocase complex.

Biological function summary

TIM22 interacts with several mitochondria-rich cellular environments to facilitate the integration of polytopic proteins. As part of the TIM22 complex it integrates endeavored proteins into the mitochondrial inner membrane. This integration is essential for the optimal function of the mitochondria. It interacts closely with other components including TIM54 and TIM18 which assist in its function within the complex.

Pathways

The TIM22 complex activities heavily contribute to the mitochondrial import pathways. It remains connected with the translocase of outer mitochondrial membrane (TOM) complex as it receives incoming proteins. TIM22 connects with TOM40 and small TIM such as TIM9 and TIM10 during the import and sorting processes. This integration ensures that proteins achieve the correct membrane insertion needed for mitochondrial function.

TIM22-related dysfunction can lead to mitochondrial-related disorders such as mitochondrial myopathy. This happens when the protein integration into the mitochondrial membrane faces disruption. Additionally deficiencies or mutations in TIM22 have links to neurodegenerative disorders where proper mitochondrial function is compromised. The interaction with other members of the mitochondrial membrane translocase complexes like TIM54 and TIM18 is important in these disorder contexts.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps (By similarity).

See full target information TIMM22

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports 34:108869 PubMed33730581

2021

The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Michelle Grace Acoba,Ebru S Selen Alpergin,Santosh Renuse,Lucía Fernández-Del-Río,Ya-Wen Lu,Oleh Khalimonchuk,Catherine F Clarke,Akhilesh Pandey,Michael J Wolfgang,Steven M Claypool

Journal of cellular biochemistry 122:626-638 PubMed33522619

2021

The transcriptional regulator Zfat is essential for maintenance and differentiation of the adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhei Ishikura,Masayoshi Nagai,Toshiyuki Tsunoda,Kensuke Nishi,Yoko Tanaka,Midori Koyanagi,Senji Shirasawa

Scientific reports 9:2822 PubMed30808977

2019

Mieap-induced accumulation of lysosomes within mitochondria (MALM) regulates gastric cancer cell invasion under hypoxia by suppressing reactive oxygen species accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Keiichiro Okuyama,Yoshihiko Kitajima,Noriyuki Egawa,Hiroshi Kitagawa,Kotaro Ito,Shinichi Aishima,Kazuyoshi Yanagihara,Tomokazu Tanaka,Hirokazu Noshiro

Stem cell reviews and reports 15:427-438 PubMed30338499

2018

Metabolic Syndrome Modulates Protein Import into the Mitochondria of Porcine Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Arash Aghajani Nargesi,Xiang-Yang Zhu,LaTonya J Hickson,Sabena M Conley,Andre J van Wijnen,Lilach O Lerman,Alfonso Eirin

American journal of human genetics 99:860-876 PubMed27693233

2016

Recurrent De Novo Dominant Mutations in SLC25A4 Cause Severe Early-Onset Mitochondrial Disease and Loss of Mitochondrial DNA Copy Number.

Applications

Unspecified application

Species

Unspecified reactive species

Kyle Thompson,Homa Majd,Cristina Dallabona,Karit Reinson,Martin S King,Charlotte L Alston,Langping He,Tiziana Lodi,Simon A Jones,Aviva Fattal-Valevski,Nitay D Fraenkel,Ann Saada,Alon Haham,Pirjo Isohanni,Roshni Vara,Inês A Barbosa,Michael A Simpson,Charu Deshpande,Sanna Puusepp,Penelope E Bonnen,Richard J Rodenburg,Anu Suomalainen,Katrin Õunap,Orly Elpeleg,Ileana Ferrero,Robert McFarland,Edmund R S Kunji,Robert W Taylor

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIM22 antibody [EPR9973]

Western blot - Anti-TIM22 antibody [EPR9973] (AB167423)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (5)

Product promise