Anti-TIM44 antibody [EPR16821] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (AB251197)

This data was developed using ab194829, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling TIM44 with ab194829 at 1/100 dilution (5μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution was used as secondary (ab97051). Counterstain : Hematoxylin.
Inset image : negative control obtained using PBS instead of ab194829. Cytoplasm staining on humanendometrial adenocarcinoma was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (AB251197)

This data was developed using ab194829, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of A431 cells labeling TIM44 with ab194829 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain : DAPI. Cytoplasm staining on A431 cell line was observed.

• IP

Supplier Data

Immunoprecipitation - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (AB251197)

This data was developed using ab194829, the same antibody clone in a different buffer formulation.

Immunoprecipitation analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab194826 at 1/20 dilution (Lane 1). Lane 2 : PBS instead of K562 cell lysate. ab194829 at 1/1000 was used for subsequent western blot detection. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-TIM44 antibody [EPR16821] (<a href='/ko/products/primary-antibodies/tim44-antibody-epr16821-ab194829'>ab194829</a>)

Predicted band size: 51 kDa

false

• WB

Supplier Data

Western blot - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (AB251197)

This data was developed using ab194829, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TIM44 antibody [EPR16821] (<a href='/ko/products/primary-antibodies/tim44-antibody-epr16821-ab194829'>ab194829</a>) at 1/5000 dilution

Lane 1:

A431 cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

false

• WB

Supplier Data

Western blot - Anti-TIM44 antibody [EPR16821] - BSA and Azide free (AB251197)

This data was developed using ab194829, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TIM44 antibody [EPR16821] (<a href='/ko/products/primary-antibodies/tim44-antibody-epr16821-ab194829'>ab194829</a>) at 1/1000 dilution

All lanes:

Mouse heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

false