AB236451

Anti-TIPIN antibody

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Rabbit Polyclonal TIPIN antibody. N-terminal. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TIMELESS-interacting protein.

대체 명칭 보기

TIMELESS-interacting protein, TIPIN

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-TIPIN antibody (AB236451)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TIPIN antibody (AB236451)

2% paraformaldehyde-fixed U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling TIPIN with ab236451 (green) at 1/600 dilution in ICC/IF. Cells were treated with siRNA against TIPIN (right panel) or control (left panel). Counter stained with DAPI (blue). Incubated with primary antibody overnight at +4°C.

Supplier Data

Immunoprecipitation - Anti-TIPIN antibody (AB236451)

TIPIN was immunoprecipitated from U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate using 3 μl of ab236451.

Lane 1 : U-2 OS whole cell lysate ~400 μg (Input).

Lane 2 : Control IgG in U-2 OS whole cell lysate.

Lane 3 : ab236451 in U-2 OS whole cell lysate.

For western blotting ab236451 was used at a 1/2000 dilution.

All lanes:

Immunoprecipitation - Anti-TIPIN antibody (ab236451)

Predicted band size: 35 kDa

false

Supplier Data

Western blot - Anti-TIPIN antibody (AB236451)

Blocking : 5% TBS-T milk.

Incubated with primary antibody overnight.

All lanes:

Western blot - Anti-TIPIN antibody (ab236451) at 1/2000 dilution

Lane 1:

U-2 OS (Human bone osteosarcoma epithelial cell line) treated with control siRNA cell lysate at 40 µg

Lane 2:

U-2 OS (Human bone osteosarcoma epithelial cell line) treated with TIPIN siRNA cell lysate at 40 µg

Lane 3:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate at 40 µg

Predicted band size: 35 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TIMELESS-interacting protein.

Q9BVW5

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/2000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/600", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purity

Whole antiserum

보관 버퍼

Preservative: 0.1% Sodium azide

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The TIPIN protein also known as Timekeeper Homolog is a 29 kDa protein involved in the regulation of DNA replication and genome stability. It interacts with other proteins to form a complex that plays a role in stabilizing the replication machinery. TIPIN expresses in various tissues including those with high proliferation rates. Researchers study its mechanical role because of its involvement in maintaining the proper functioning of the replication fork a critical part of DNA synthesis.

Biological function summary

The interaction of TIPIN in the replication fork protection complex is important for preventing genomic instability. TIPIN associates with the Timeless protein to form an important complex that maintains the integrity of DNA replication under stress conditions. This complex stabilizes replication forks and prevents DNA breakage allowing smooth progression of DNA synthesis. Without its function cells might encounter failed replication processes and increased mutations.

Pathways

TIPIN integrates into the DNA damage response pathway which maintains genome integrity by activating repair processes. It interacts with the ATR-Chk1 signaling pathway an important mediator of replication stress response. This pathway triggers cell cycle arrest to give cells time to repair damaged DNA. TIPIN collaborates with Timeless and other proteins like MCM helicase preventing replication collapse and maintaining genomic stall points.

TIPIN's role in maintaining genomic stability connects it with cancer development where DNA replication errors can promote tumorigenesis. Changes or mutations affecting TIPIN expression can contribute to breast cancer. Additionally TIPIN is linked to ATR mutations in rare genetic disorders like Seckel syndrome where defective DNA repair causes developmental problems. The relationship between TIPIN and these proteins highlights its possible impact on such diseases serving as a target for therapeutic interventions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays an important role in the control of DNA replication and the maintenance of replication fork stability (PubMed : 17102137, PubMed : 23359676, PubMed : 35585232). Important for cell survival after DNA damage or replication stress (PubMed : 17116885). May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light (PubMed : 17296725). Forms a complex with TIMELESS and this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress (PubMed : 17102137, PubMed : 17116885, PubMed : 17296725, PubMed : 23359676, PubMed : 35585232).

See full target information TIMELESS-interacting protein

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

British journal of pharmacology 182:3594-3611 PubMed40194519

2025

Inhibition of DNA polymerase eta-mediated translesion DNA synthesis with small molecule sensitises ovarian cancer stem-like cells to chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Subhankar Bose,Priyanka Saha,Md Tanjim Alam,Bilash Chatterjee,Mrinmoy Sarkar,Amit Kumar Dixit,Deepak Kumar,Rakesh Kumar Pathak,Prem Prakash Tripathi,Amit Kumar Srivastava

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIPIN antibody

Immunocytochemistry/ Immunofluorescence - Anti-TIPIN antibody (AB236451)

Immunoprecipitation - Anti-TIPIN antibody (AB236451)

Western blot - Anti-TIPIN antibody (AB236451)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purity

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise