JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB309243

Anti-TIRAP antibody [EPR27962-69] - BSA and Azide free (Detector)

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Rabbit Recombinant Monoclonal TIRAP antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

대체 명칭 보기

MAL, TIRAP, Toll/interleukin-1 receptor domain-containing adapter protein, TIR domain-containing adapter protein, Adaptor protein Wyatt, MyD88 adapter-like protein, MyD88-2

1 이미지
Sandwich ELISA - Anti-TIRAP antibody [EPR27962-69] - BSA and Azide free (Detector) (AB309243)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-TIRAP antibody [EPR27962-69] - BSA and Azide free (Detector) (AB309243)

Sandwich ELISA of ab309273 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27962-69

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

보완 제품

Primary Antibodies

AB17218

Anti-TIRAP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIRAP antibody (AB17218)

  • 5
  • 2
Primary Antibodies

AB183517

Anti-TRRAP antibody [EPR14952]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-TRRAP antibody [EPR14952] (AB183517)

  • 0
  • 0
Primary Antibodies

AB46010

Anti-CXCL14 antibody

Sandwich ELISA - Anti-CXCL14 antibody (AB46010)

  • 0
  • 0
Primary Antibodies

AB203102

Anti-ADAMTS12 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAMTS12 antibody (AB203102)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

특성 및 보관 정보

제형
Liquid
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TIRAP also known as Mal is an adapter protein integral to the Toll-like receptor (TLR) signaling pathway. It has a molecular mass of approximately 24 kDa. TIRAP resides mostly in the cytoplasm and gets recruited to the plasma membrane where it assists in signal transduction. It plays a significant role in bridging TLRs such as TLR2 and TLR4 with downstream signaling elements like MyD88. This protein mediates the recruitment of other signaling proteins after TLR activation therefore facilitating the innate immune response.
Biological function summary

TIRAP is part of the innate immune system and contributes to the immune response against pathogens. It forms part of a signaling complex that leads to the activation of NF-kB and MAP kinase pathways resulting in the production of inflammatory cytokines. The protein works by directly interacting with phosphatidylinositol 45-bisphosphate at the plasma membrane through its phosphoinositide-binding motif. This interaction ensures that TIRAP is correctly positioned to relay signals for immune activation.

Pathways

TIRAP functions in the MyD88-dependent signaling pathway which is significant for initiating inflammatory responses. TIRAP and MyD88 work in tandem to propagate signals from activated TLRs. This relationship not only aids in inflammation but also in the differentiation of immune cells. TIRAP is also related to other proteins like IRAK1 in these pathways which further transduces signals leading to downstream activation of transcription factors relevant for immune responses.

TIRAP plays a part in conditions like systemic lupus erythematosus and septic shock. Mutations in the TIRAP gene can alter the signaling cascade leading to aberrant immune responses associated with these diseases. In this context TIRAP interacts with TLR4 and any dysfunction in the interaction may contribute to the excessive inflammation seen in septic shock. Understanding TIRAP's role in these diseases provides insight into targeted therapeutic strategies.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Adapter involved in TLR2, TLR4 and RAGE signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF (TNF) and interleukin-6 (IL6).
See full target information TIRAP
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com