Anti-Tissue kallikrein antibody [EPR25709-526]

• WB

Supplier Data

Western blot - Anti-Tissue kallikrein antibody [EPR25709-526] (AB323796)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands lower than 40 kDa are predicted to be glycosylated isoforms.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Tissue kallikrein antibody [EPR25709-526] (ab323796) at 1/1000 dilution

Lane 1:

Untreated human salivary gland tissue lysate at 20 µg

Lane 2:

Human salivary gland tissue lysate treated with Protein Deglycosylation MIX II at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40 kDa,29 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Tissue kallikrein antibody [EPR25709-526] (AB323796)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Human lung and testis.

The identity of the bands lower than 40 kDa are predicted to be glycosylated isoforms.

The identity of the bands higher than 70 kDa is unkown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Tissue kallikrein antibody [EPR25709-526] (ab323796) at 1/1000 dilution

Lane 1:

Human salivary gland tissue lysate at 20 µg

Lane 2:

Human pancreas tissue lysate at 20 µg

Lane 3:

Human lung tissue lysate at 20 µg

Lane 4:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40 kDa,36 kDa

false

Exposure time: 81s

• Dot

Supplier Data

Dot Blot - Anti-Tissue kallikrein antibody [EPR25709-526] (AB323796)

Dot blot analysis of Tissue kallikrein using ab323796 at 1 : 1000 (0.49 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : LIF-tagged human KLK1 fragment
Lane2 : LIF-tagged human KLK2 fragment
Lane3 : LIF-tagged human KLK3 fragment

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Dot Blot, Anti-LIF antibody [EPR21713-25] - BSA and Azide free (Capture) (ab244795) staining at 1 : 1000 dilution.

All lanes:

Dot Blot - Anti-Tissue kallikrein antibody [EPR25709-526] (ab323796) at 1/1000 dilution

Lane 1:

LIF-tagged human KLK1 fragment

Lane 2:

LIF-tagged human KLK2 fragment

Lane 3:

LIF-tagged human KLK3 fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s