AB228962

Anti-TLR8 antibody [EPR22848-74]

BOND RX™ Validated
RabMAb
Recombinant
각 태그의 뜻

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal TLR8 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications.

대체 명칭 보기

CD288, UNQ249/PRO286, TLR8, Toll-like receptor 8

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR8 antibody [EPR22848-74] (AB228962)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR8 antibody [EPR22848-74] (AB228962)

Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue labeling TLR8 with ab228962 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human B-cell lymphoma (PMID : 20800061) is observed. The section was incubated with ab228962 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR8 antibody [EPR22848-74] (AB228962)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling TLR8 with ab228962 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human spleen (PMID : 20811154) is observed. The section was incubated with ab228962 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Lab

Western blot - Anti-TLR8 antibody [EPR22848-74] (AB228962)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 59 secs.

All lanes:

Western blot - Anti-TLR8 antibody [EPR22848-74] (ab228962) at 1/1000 dilution

Lane 1:

THP-1 (human monocytic leukemia monocyte), treated with 80nM 12-O-Tetradecanoylphorbol-13-acetate (TPA) for 16 hours, whole cell lysate at 20 µg

Lane 2:

THP-1 treated with 80nM 12-O-Tetradecanoylphorbol-13-acetate (TPA) for 16 hours, then with 20ng/ml Human interferon gamma for 8 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 120 kDa

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22848-74

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TLR8 also known as Toll-like receptor 8 is a member of the Toll-like receptor family and has a molecular mass of approximately 120 kDa. This receptor is mainly expressed in immune cells such as monocytes macrophages and dendritic cells. The receptor is located within endosomes and is responsible for recognizing single-stranded RNA (ssRNA) from viruses which leads to an immune response. TLR8 plays a part in detecting pathogens and activating innate immunity.

Biological function summary

TLR8 contributes to the immune system by playing a role in the innate immune response specifically in sensing viral components. TLR8 forms part of a larger receptor complex that includes MyD88 (myeloid differentiation primary response 88) an adaptor protein important for signaling processes within innate immunity. Upon recognizing ssRNA TLR8 initiates signaling cascades to trigger the release of inflammatory cytokines and other mediators that contribute to an antiviral response.

Pathways

TLR8 engages in the innate immune signaling pathway and the NF-kB pathway. These pathways are significant for the activation of immune responses and inflammation. MyD88 bridges TLR8 and downstream signaling events leading to the production and release of pro-inflammatory cytokines. In addition to MyD88 connections to molecules like IRAK4 illustrate how TLR8 signaling maintains a robust immune response to pathogen detection.

TLR8 is linked to autoimmune conditions and viral infections. The receptor's activation by viral components can contribute to chronic inflammation seen in autoimmune diseases such as systemic lupus erythematosus. Additionally aberrant TLR8 signaling is associated with increased susceptibility to certain viral infections where its relationship with MyD88 influences both protective and pathological responses in diseases. Understanding TLR8's function may unveil new therapeutic approaches to manage immune-related disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Endosomal receptor that plays a key role in innate and adaptive immunity (PubMed : 25297876, PubMed : 32433612). Controls host immune response against pathogens through recognition of RNA degradation products specific to microorganisms that are initially processed by RNASET2 (PubMed : 31778653). Recognizes GU-rich single-stranded RNA (GU-rich RNA) derived from SARS-CoV-2, SARS-CoV-1 and HIV-1 viruses (PubMed : 33718825). Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction (PubMed : 23520111, PubMed : 25599397, PubMed : 26929371, PubMed : 33718825). In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons, respectively (PubMed : 16737960, PubMed : 17932028, PubMed : 29155428).

See full target information TLR8

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in immunology 15:1480675 PubMed39544932

2024

Possible involvement of Toll-like receptor 8-positive monocytes/macrophages in the pathogenesis of Sjögren's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Lijing Yan,Yuka Miyahara,Mizuki Sakamoto,Naoki Kaneko,Hu Chen,Junsei Sameshima,Hajime Kido,Shiho Yokomizo,Tomoki Sueyoshi,Haruki Nagano,Yukiko Ohyama,Seiji Nakamura,Shintaro Kawano,Masafumi Moriyama

Cell death discovery 9:315 PubMed37626060

2023

Neutrophil extracellular traps contribute to immunothrombosis formation via the STING pathway in sepsis-associated lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Shuainan Zhu,Ying Yu,Mengdi Qu,Zhiyun Qiu,Hao Zhang,Changhong Miao,Kefang Guo

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TLR8 antibody [EPR22848-74]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR8 antibody [EPR22848-74] (AB228962)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR8 antibody [EPR22848-74] (AB228962)

Western blot - Anti-TLR8 antibody [EPR22848-74] (AB228962)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise