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AB62577

Anti-TLR9 antibody

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(1 출판물)

Rabbit Polyclonal TLR9 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TLR9.
2 이미지
Immunocytochemistry - Anti-TLR9 antibody (AB62577)
  • ICC

Unknown

Immunocytochemistry - Anti-TLR9 antibody (AB62577)

ab62577 at 2μg/ml staining TLR9 in Jurkat cells by Immunocytochemistry.

Western blot - Anti-TLR9 antibody (AB62577)
  • WB

Unknown

Western blot - Anti-TLR9 antibody (AB62577)

Lane 1:

Western blot - Anti-TLR9 antibody (ab62577) at 0.5 µg/mL

Lane 2:

Western blot - Anti-TLR9 antibody (ab62577) at 1 µg/mL

Lane 3:

Western blot - Anti-TLR9 antibody (ab62577) at 2 µg/mL

All lanes:

Jurkat cell lysate at 15 µg

Predicted band size: 115 kDa

Observed band size: 95 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC

applications

Immunogen

Synthetic Peptide within Human TLR9. The exact immunogen used to generate this antibody is proprietary information.

Q9NR96

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대체품

Primary Antibodies

AB37154

Anti-TLR9 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody (AB37154)

  • 5
  • 1
Primary Antibodies

AB58864

FITC Anti-TLR9 antibody [5G5]

Flow Cytometry (Intracellular) - FITC Anti-TLR9 antibody [5G5] (AB58864)

  • 0
  • 0
Primary Antibodies

AB211012

Anti-TLR9 antibody [EPR21735]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunoprecipitation - Anti-TLR9 antibody [EPR21735] (AB211012)

  • 0
  • 0
Primary Antibodies

AB187148

Anti-TLR9 antibody [EPR14964-2]

20ul selling size|BOND RX™ Validated|RabMAb|KO Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR9 antibody [EPR14964-2] (AB187148)

  • 0
  • 0
Primary Antibodies

AB134368

Anti-TLR9 antibody [26C593.2]

Western blot - Anti-TLR9 antibody [26C593.2] (AB134368)

  • 0
  • 0
Primary Antibodies

AB232933

Anti-TLR9 antibody [EPR21735] - BSA and Azide free

RabMAb|KO Validated|Recombinant

Immunoprecipitation - Anti-TLR9 antibody [EPR21735] - BSA and Azide free (AB232933)

  • 0
  • 0

보완 제품

Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Primary Antibodies

AB219413

Anti-MyD88 antibody [EPR21824]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunoprecipitation - Anti-MyD88 antibody [EPR21824] (AB219413)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB22048

Anti-TLR4 antibody [76B357.1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR4 antibody [76B357.1] (AB22048)

  • 4
  • 29
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TLR9 also known as Toll-like receptor 9 is a protein that detects unmethylated CpG motifs which are common in bacterial and viral DNA. This protein weighs about 116 kDa. It mainly expresses in plasmacytoid dendritic cells B cells and in epithelial cells of various tissues. TLR9 locates in the endoplasmic reticulum and translocates to endolysosomes upon activation which is essential for its detection function.
Biological function summary

TLR9 recognizes and binds to microbial DNA triggering an immune response. It is part of the Toll-like receptor family which plays an important role in the innate immune system. TLR9 engages in the formation of complexes with other molecules such as MyD88 an adaptor protein to propagate downstream signaling. This leads to the activation of transcription factors that stimulate cytokine production promoting inflammation and defense against infections.

Pathways

TLR9 participates significantly in the NF-kB activation pathway and the type I interferon signaling cascade. During these processes TLR9 interacts with proteins like IRAK4 and TRAF6. In the NF-kB pathway TLR9's role in the early immune response is coordinated with other Toll-like receptors to detect pathogen-related patterns and initiate adaptive immunity. Its influence on type I interferon signaling facilitates antiviral defense mechanisms.

TLR9's malfunction or altered expression associates with systemic lupus erythematosus (SLE) and cancer. In SLE overactivation of TLR9 leads to autoantibody production which worsens the disease. This can involve the protein BAFF which supports B cell proliferation. In cancer TLR9 can influence tumor growth and the tumor microenvironment affecting disease progression. Its modulation is being researched for therapeutic interventions in these conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Key component of innate and adaptive immunity (PubMed : 14716310). TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms (PubMed : 14716310). TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides (PubMed : 14716310). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 11564765, PubMed : 17932028). Also acts via ADCY7, leading to cyclic di-AMP (c-di-AMP) synthesis and activation of the NLRP3 inflammasome (By similarity). Plays a role in defense against systemic mouse cytomegalovirus infection (By similarity). Controls lymphocyte response to Helicobacter infection (By similarity). Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production (PubMed : 23857366).
See full target information TLR9

대체 명칭 보기

CD289, UNQ5798/PRO19605, TLR9, Toll-like receptor 9
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Proceedings of the National Academy of Sciences of 112:E1659-68 PubMed25775528

2015

Heteromerization of chemokine (C-X-C motif) receptor 4 with α1A/B-adrenergic receptors controls α1-adrenergic receptor function.

Applications

Unspecified application

Species

Unspecified reactive species

Abhishek Tripathi,P Geoff Vana,Tanmay S Chavan,Lioubov I Brueggemann,Kenneth L Byron,Nadya I Tarasova,Brian F Volkman,Vadim Gaponenko,Matthias Majetschak
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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