AB241992

Anti-TMEM109/Mitsugumin-23 antibody

Be the first to review this product! 리뷰 제출

(1 출판물)

Rabbit Polyclonal TMEM109/Mitsugumin-23 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TMEM109 aa 1-100.

대체 명칭 보기

Voltage-gated monoatomic cation channel TMEM109, Mitsugumin-23, Transmembrane protein 109, Mg23, TMEM109

2 이미지

Supplier Data

Immunoprecipitation - Anti-TMEM109/Mitsugumin-23 antibody (AB241992)

TMEM109/Mitsugumin-23 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241992 at 20 μL per reaction. Western blot was performed from the immunoprecipitate using ab241992 at 1/100 dilution.

Lane 1 : ab241992 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds.

Prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-TMEM109/Mitsugumin-23 antibody (ab241992)

Predicted band size: 26 kDa

false

Supplier Data

Western blot - Anti-TMEM109/Mitsugumin-23 antibody (AB241992)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-TMEM109/Mitsugumin-23 antibody (ab241992) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 26 kDa

true

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human TMEM109 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9BVC6

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "20 μl/mg lysate", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antibody was affinity purified using an epitope specific to TMEM109/Mitsugumin-23 immobilized on solid support.

보관 버퍼

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TMEM109 also known as Mitsugumin-23 (MG23) functions as a transmembrane protein with a mass of approximately 20-25 kDa. It resides in the endoplasmic reticulum membrane where it acts as an important component in maintaining cellular homeostasis. This protein is primarily observed across various tissues with a higher expression in muscle tissue and certain types of epithelial cells. Its structure suggests roles in calcium signaling due to domains that allow ion passage across cellular membranes.

Biological function summary

TMEM109 plays a role in the regulation of calcium ion concentrations within cells contributing to the mitochondrial permeability transition pore (MPTP) complex. This complex controls mitochondrial function by regulating the passage of ions and small metabolites which is critical for cell survival and apoptosis. Through these activities TMEM109 influences processes like muscle contraction and cell death highlighting its significance in cellular energy management and programmed cell death pathways.

Pathways

TMEM109 has roles in calcium signaling and apoptotic pathways two significant biological processes for cellular health. In the calcium signaling pathway it interacts with other proteins such as SERCA1 (Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 1) to help manage calcium ion levels within cells. In apoptotic pathways TMEM109's involvement in the MPTP complex links it to the BAX protein which is an essential regulator of apoptosis facilitating the release of cytochrome c from mitochondria triggering cascade events leading to cell death.

Irregular function or expression of TMEM109 associates with conditions such as muscular dystrophy and neurodegenerative disorders. For example in muscular dystrophy altered calcium regulation and signaling through MG23 may impact muscle contraction and integrity. Additionally abnormal TMEM109 activity can connect to Alzheimer’s disease where disrupted calcium homeostasis and apoptotic signaling play roles. These disease connections make TMEM109 a considerable target for therapeutic research and intervention often intersecting pathways with proteins like dystrophin in muscular dystrophy cases and amyloid precursor protein (APP) in Alzheimer's disease.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Functions as a voltage-gated monoatomic cation channel permeable to both potassium and calcium (By similarity). Plays a role in the cellular response to DNA damage (PubMed : 23542032).

See full target information TMEM109

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of cellular and molecular medicine : PubMed32757436

2020

Quantitative proteomics identified 3 oxidative phosphorylation genes with clinical prognostic significance in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Su,Fen-Fang Zhou,Tao Zhang,Dan-Wen Wang,Da Zhao,Xiao-Ming Hou,Mao-Hui Feng

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TMEM109/Mitsugumin-23 antibody

Immunoprecipitation - Anti-TMEM109/Mitsugumin-23 antibody (AB241992)

Western blot - Anti-TMEM109/Mitsugumin-23 antibody (AB241992)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise