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AB325668

Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric)

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Chicken Recombinant Monoclonal tmem119 antibody. Suitable for IHC-P, WB and reacts with Rat, Transfected cell lysate samples.
3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (AB325668)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (AB325668)

Representative section of formalin fixed, paraffin-embedded rat brain stem showing microglia cells stained by ab325668 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. Magnification 100X.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (AB325668)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (AB325668)

Representative section of formalin fixed, paraffin-embedded rat brain stem showing microglia cells stained by ab325668 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. Magnification 400X.

Western blot - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (AB325668)
  • WB

Supplier Data

Western blot - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (AB325668)

Western blotting of mock transfected and TMEM119-FLAG transfected COS-7 lysates with ab325668 (10 µg/mL) and detected with anti-chicken-HRP. The antibody recognizes exogenous TMEM119 only in TMEM119-FLAG transfected COS-7 lysates at 40 kDa.

All lanes:

Western blot - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (ab325668) at 10 µg/mL

Lane 1:

mock transfected COS-7 lysates

Lane 2:

TMEM119-FLAG transfected COS-7 lysates

Secondary

All lanes:

anti-chicken-HRP

Observed band size: 40 kDa

false

주요 정보

Host species

Chicken

Clonality

Monoclonal

Clone number

L128/43

Isotype

IgY

Carrier free

No

Reacts with

Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>No cross-reactivity reported.</p>

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보완 제품

Primary Antibodies

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Anti-TMEM119 antibody [28-3] - Microglial marker

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Immunohistochemistry (Frozen sections) - Anti-TMEM119 antibody [28-3] - Microglial marker (AB209064)

  • 4
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Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 50
Primary Antibodies

AB274386

Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P2Y12 antibody [EPR23511-72] - BSA and Azide free (AB274386)

  • 2
  • 3
Primary Antibodies

AB300421

Anti-F4/80 antibody [EPR26545-166]

BOND RX™ Validated|Recombinant|Lab Essentials|20ul selling size|RabMAb|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)

  • 5
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10 µg/mL", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L128/43 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L128/43 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
보관 버퍼
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

See full target information tmem119
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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