AB236858

Anti-TMEM192 antibody

Be the first to review this product! 리뷰 제출

(1 출판물)

Rabbit Polyclonal TMEM192 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TMEM192 aa 150-300.

대체 명칭 보기

Transmembrane protein 192, TMEM192

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-TMEM192 antibody (AB236858)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TMEM192 antibody (AB236858)

A549 (human lung carcinoma cell line) cells stained for TMEM192 (green) using ab236858 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L).

The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM192 antibody (AB236858)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM192 antibody (AB236858)

Paraffin-embedded human adrenal gland tissue stained for TMEM192 using ab236858 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM192 antibody (AB236858)

Paraffin-embedded human glioma tissue stained for TMEM192 using ab236858 at 1/400 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TMEM192 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q8IY95

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/500", "ICCIF-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TMEM192 also known as Transmembrane Protein 192 has a molecular weight of approximately 25 kDa. It is a lysosomal membrane protein expressed in various tissues including the spleen and thymus. This protein predominantly localizes to the lysosomal compartment where it plays a role in maintaining lysosomal integrity. Researchers often study TMEM192 for its role in membrane stabilization and its interaction with other lysosomal proteins.

Biological function summary

TMEM192 participates in the regulation of lysosomal functions. It integrates into the lysosomal membrane and contributes to the stability and function of this organelle. While not commonly part of larger protein complexes it influences lysosomal enzymatic activities by stabilizing the lysosomal environment. Its role in regulating pH and transport processes within the lysosome directly affects how the cell processes cellular debris and macromolecules.

Pathways

TMEM192 interacts with cellular processes related to lysosomal biogenesis and autophagy. It functions closely with lysosomal-associated membrane protein 1 (LAMP1) within the lysosomal pathway which plays a critical role in the degradation and recycling of cellular components. The lysosomal pathway where TMEM192 operates is significant for maintaining cellular homeostasis by regulating autophagic activity and responding to nutrient availability.

Disruptions in TMEM192 function associate with lysosomal storage disorders. Altered expression or mutations in TMEM192 link to Niemann-Pick disease type C which involves the accumulation of lipids within lysosomes. LAMP1 a protein associated with TMEM192 also plays a part in this disease pathway contributing to understanding the mechanism of lysosomal dysregulation in Niemann-Pick and similar disorders.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

See full target information TMEM192

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Experimental dermatology 31:1253-1263 PubMed35514241

2022

Silencing of Rab23 by siRNA inhibits ultraviolet B-induced melanogenesis via downregulation of PKA/CREB/MITF.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanjin Wei,Min Huang,Yi Yang,Yali Liu,Sumin Chi,Chengxin Li

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TMEM192 antibody

Immunocytochemistry/ Immunofluorescence - Anti-TMEM192 antibody (AB236858)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM192 antibody (AB236858)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM192 antibody (AB236858)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise