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AB238740

Anti-TMEM2 antibody

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Rabbit Polyclonal TMEM2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CEMIP2 aa 700-900.

대체 명칭 보기

KIAA1412, TMEM2, CEMIP2, Cell surface hyaluronidase CEMIP2, Cell migration-inducing hyaluronidase 2, Transmembrane protein 2

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-TMEM2 antibody (AB238740)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TMEM2 antibody (AB238740)

A549 (Human lung carcinoma cell line) cells stained for TMEM2 (Green) using ab238740 at 1/100 dilution in ICC/IF, followed by an Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. The nuclear counterstain is DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM2 antibody (AB238740)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM2 antibody (AB238740)

Paraffin-embedded human brain tissue stained for TMEM2 using ab238740 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CEMIP2 aa 700-900. The exact immunogen used to generate this antibody is proprietary information.

Q9UHN6

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보완 제품

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB302710

Anti-PARP14 antibody [EPR26299-91]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-PARP14 antibody [EPR26299-91] (AB302710)

  • 5
  • 1
Primary Antibodies

AB183724

Anti-N myc interactor/NMI antibody [EPR11065(2)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N myc interactor/NMI antibody [EPR11065(2)] (AB183724)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity greater than 95%.
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TMEM2 also known as Transmembrane Protein 2 is a protein involved in the hydrolysis of hyaluronan a component of the extracellular matrix. TMEM2 is a single-pass type II transmembrane protein with a molecular mass of approximately 150 kDa. It is expressed in various tissues with heightened expression in the heart skeletal muscle and connective tissues. Researchers have noted its importance in the remodeling of the matrix during developmental processes and tissue repair.
Biological function summary

The protein TMEM2 plays a role in hyaluronan turnover which affects processes like cell motility and proliferation. It is not part of a larger protein complex but its activity influences the availability of hyaluronan fragments that interact with other cell surface receptors. This interaction can influence cellular responses such as inflammation and tissue modeling.

Pathways

TMEM2 is significant in the matrix degradation pathway impacting cellular and tissue development signaling. It is related to the extracellular matrix organization pathway where its function in hyaluronan degradation directly affects other matrix proteins. This interaction is often seen with proteins such as CD44 and RHAMM which are known to bind hyaluronan fragments and mediate further signaling.

TMEM2 mutations or dysfunctions have links to cardiovascular diseases and certain cancers. Its role in hyaluronan turnover has implications in pathological processes like tumor metastasis given that altered extracellular matrix can affect cancer cell mobility. The protein interacts with CD44 a receptor involved in tumor progression and metastasis highlighting its potential importance in oncological research. Understanding TMEM2 and its pathways could provide insights into therapeutic targets for treating cancer and cardiovascular conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Cell surface hyaluronidase that mediates the initial cleavage of extracellular high-molecular-weight hyaluronan into intermediate-size hyaluronan of approximately 10-5 kDa fragments (PubMed : 37527776). Very specific to hyaluronan; not able to cleave chondroitin sulfate or dermatan sulfate. Has an essential function in systemic hyaluronan catabolism and turnover and regulates cell adhesion and migration via hyaluronan degradation at focal adhesion sites (By similarity). Acts as a regulator of angiogenesis and heart morphogenesis by mediating degradation of extracellular hyaluronan, thereby regulating VEGF signaling (By similarity).
See full target information CEMIP2
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Bioengineered 12:5323-5333 PubMed34499008

2021

The tumor-suppressive effects of alpha-ketoglutarate-dependent dioxygenase FTO via N6-methyladenosine RNA methylation on bladder cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Wenfa Yi,Yong Yu,Yafei Li,Juan Yang,Shuying Gao,Lingfen Xu
제품이 사용된 논문 모두 보기
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Product promise

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