AB272644

Anti-TMEM2 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal TMEM2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CEMIP2 aa 1-100.

대체 명칭 보기

KIAA1412, TMEM2, CEMIP2, Cell surface hyaluronidase CEMIP2, Cell migration-inducing hyaluronidase 2, Transmembrane protein 2

6 이미지

Immunocytochemistry/ Immunofluorescence - Anti-TMEM2 antibody (AB272644)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TMEM2 antibody (AB272644)

PFA fixed, Triton X-100 permeablized U-2 OS cells (human bone osteosarcoma epithelial cell line) labeling TMEM2 (green) using ab272644 at 2 ug/ml in ICC/IF analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM2 antibody (AB272644)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM2 antibody (AB272644)

Paraffin-embedded formalin-fixed human skeletal tissue stained for TMEM2 using ab272644 at 1/50 dilution in immunohistochemical analysis. Shows no expression as expected.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM2 antibody (AB272644)

Paraffin-embedded formalin-fixed human placenta tissue stained for TMEM2 using ab272644 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM2 antibody (AB272644)

Paraffin-embedded formalin-fixed human small intestine tissue stained for TMEM2 using ab272644 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM2 antibody (AB272644)

Paraffin-embedded formalin-fixed human Fallopian tube tissue stained for TMEM2 using ab272644 at 1/50 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-TMEM2 antibody (AB272644)

All lanes:

Western blot - Anti-TMEM2 antibody (ab272644) at 0.4 µg/mL

Lane 1:

A549 cells (Human lung carcinoma cell line)

Lane 2:

PC-3 cells (Human prostate adenocarcinoma cell line)

Predicted band size: 154 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human CEMIP2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9UHN6

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TMEM2 also known as Transmembrane Protein 2 is a protein involved in the hydrolysis of hyaluronan a component of the extracellular matrix. TMEM2 is a single-pass type II transmembrane protein with a molecular mass of approximately 150 kDa. It is expressed in various tissues with heightened expression in the heart skeletal muscle and connective tissues. Researchers have noted its importance in the remodeling of the matrix during developmental processes and tissue repair.

Biological function summary

The protein TMEM2 plays a role in hyaluronan turnover which affects processes like cell motility and proliferation. It is not part of a larger protein complex but its activity influences the availability of hyaluronan fragments that interact with other cell surface receptors. This interaction can influence cellular responses such as inflammation and tissue modeling.

Pathways

TMEM2 is significant in the matrix degradation pathway impacting cellular and tissue development signaling. It is related to the extracellular matrix organization pathway where its function in hyaluronan degradation directly affects other matrix proteins. This interaction is often seen with proteins such as CD44 and RHAMM which are known to bind hyaluronan fragments and mediate further signaling.

TMEM2 mutations or dysfunctions have links to cardiovascular diseases and certain cancers. Its role in hyaluronan turnover has implications in pathological processes like tumor metastasis given that altered extracellular matrix can affect cancer cell mobility. The protein interacts with CD44 a receptor involved in tumor progression and metastasis highlighting its potential importance in oncological research. Understanding TMEM2 and its pathways could provide insights into therapeutic targets for treating cancer and cardiovascular conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cell surface hyaluronidase that mediates the initial cleavage of extracellular high-molecular-weight hyaluronan into intermediate-size hyaluronan of approximately 10-5 kDa fragments (PubMed : 37527776). Very specific to hyaluronan; not able to cleave chondroitin sulfate or dermatan sulfate. Has an essential function in systemic hyaluronan catabolism and turnover and regulates cell adhesion and migration via hyaluronan degradation at focal adhesion sites (By similarity). Acts as a regulator of angiogenesis and heart morphogenesis by mediating degradation of extracellular hyaluronan, thereby regulating VEGF signaling (By similarity).

See full target information CEMIP2

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

American journal of cancer research 14:3010-3035 PubMed39005682

2024

analysis of TMEM2 as a pancreatic adenocarcinoma and cancer-associated fibroblast biomarker, and functional characterization of NSC777201, for targeted drug development.

Applications

Unspecified application

Species

Unspecified reactive species

Prateeti Srivastava,Vijesh Kumar Yadav,Tzu-Hao Chang,Emily Chia-Yu Su,Bashir Lawal,Alexander Th Wu,Hsu-Shan Huang

Heliyon 9:e16559 PubMed37292284

2023

TMEM2 induces epithelial-mesenchymal transition and promotes resistance to temozolomide in GBM cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lun Gao,Shiao Tong,Junhui Liu,Jiayang Cai,Zhang Ye,Long Zhou,Ping Song,Zhiyang Li,Pan Lei,Hangyu Wei,Qiuwei Hua,Daofeng Tian,Qiang Cai

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TMEM2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-TMEM2 antibody (AB272644)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM2 antibody (AB272644)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM2 antibody (AB272644)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM2 antibody (AB272644)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM2 antibody (AB272644)

Western blot - Anti-TMEM2 antibody (AB272644)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise