AB128006

Anti-TMEPAI antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal TMEPAI antibody. Suitable for IHC-P, ELISA, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PMEPA1.

대체 명칭 보기

STAG1, TMEPAI, PMEPA1, Protein TMEPAI, Prostate transmembrane protein androgen induced 1, Solid tumor-associated 1 protein, Transmembrane prostate androgen-induced protein

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEPAI antibody (AB128006)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEPAI antibody (AB128006)

ab128006, at a concentration of 10 µg/ml, staining TMEPAI in paraffin-embedded Human Prostate, Smooth Muscle tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-TMEPAI antibody (AB128006)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TMEPAI antibody (AB128006)

ab128006, at a dilution of 1/100, staining TMEPAI in HuvEc cells by Immunofluorescence. The picture on the right is treated with the synthesized peptide.

Unknown

Western blot - Anti-TMEPAI antibody (AB128006)

All lanes:

Western blot - Anti-TMEPAI antibody (ab128006) at 1/100 dilution

Lane 1:

HT29 cell extract

Lane 2:

HT29 cell extract with synthesized peptide

Predicted band size: 32 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF, ELISA

applications

Immunogen

Synthetic Peptide within Human PMEPA1. The exact immunogen used to generate this antibody is proprietary information.

Q969W9

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TMEPAI also known as TMPRSS6-associated product containing transcript is a transmembrane protein. It has a molecular weight of approximately 26 kDa. TMEPAI is widely expressed in various tissues with particularly high expression in the lungs prostate and breast. It interacts with the cytoplasmic signaling proteins through its PY motifs modulating their activities directly. TMEPAI plays an important role in cellular pathways affecting cellular functions and interactions.

Biological function summary

This protein modulates signaling pathways and cell growth regulation. It is well known for its involvement in the TGF-beta (Transforming Growth Factor-beta) signaling pathway. TMEPAI acts as a negative regulator inhibiting TGF-beta's tumor-suppressive functions through interaction with Smad proteins. While it does not form part of a complex itself it significantly influences the functional dynamics of the TGF-beta pathway constituents impacting cellular proliferation and survival.

Pathways

TMEPAI exerts its functional significance primarily within the TGF-beta and PI3K/Akt signaling pathways. In the TGF-beta pathway TMEPAI modulates the signaling cascade by interacting with Smad2/3 and Smad4. The regulation of these proteins by TMEPAI suppresses TGF-beta-induced growth inhibition. In the PI3K/Akt pathway TMEPAI influences cell survival and proliferation indirectly through these interactions positioning it as an important modulator in these pathways.

TMEPAI has significant implications in cancer particularly in breast and prostate cancer. It facilitates cancer progression by overriding the tumor-suppressive effects of TGF-beta signaling. The interactions with Smad proteins like Smad2/3 highlight its role in oncogenic pathways. Moreover in prostate cancer the dysregulation of TMEPAI links with increased aggressive behavior of cancer cells exacerbating disease progression. Understanding these interactions provides insights into potential targets for therapeutic intervention in these diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Functions as a negative regulator of TGF-beta signaling and thereby probably plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production and immunosuppression. In the canonical TGF-beta pathway, ZFYVE9/SARA recruits the intracellular signal transducer and transcriptional modulators SMAD2 and SMAD3 to the TGF-beta receptor. Phosphorylated by the receptor, SMAD2 and SMAD3 then form a heteromeric complex with SMAD4 that translocates to the nucleus to regulate transcription. Through interaction with SMAD2 and SMAD3, LDLRAD4 may compete with ZFYVE9 and SMAD4 and prevent propagation of the intracellular signal (PubMed : 20129061, PubMed : 24627487). Also involved in down-regulation of the androgen receptor (AR), enhancing ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4 (PubMed : 18703514).

See full target information PMEPA1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33:4365-4375 PubMed30557043

2018

Prostate transmembrane protein androgen induced 1 is induced by activation of osteoclasts and regulates bone resorption.

Applications

Unspecified application

Species

Unspecified reactive species

Xianghe Xu,Hirohito Hirata,Makoto Shiraki,Asana Kamohara,Kenichi Nishioka,Hiroshi Miyamoto,Toshio Kukita,Akiko Kukita

Gastroenterology 148:1427-37.e8 PubMed25736321

2015

Transforming Growth Factor β Signaling in Colorectal Cancer Cells With Microsatellite Instability Despite Biallelic Mutations in TGFBR2.

Applications

Unspecified application

Species

Unspecified reactive species

Noel F C C de Miranda,Maarten van Dinther,Brendy E W M van den Akker,Tom van Wezel,Peter ten Dijke,Hans Morreau

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TMEPAI antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEPAI antibody (AB128006)

Immunocytochemistry/ Immunofluorescence - Anti-TMEPAI antibody (AB128006)

Western blot - Anti-TMEPAI antibody (AB128006)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise