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AB34674

Anti-TNF alpha antibody

4

(5 리뷰들)

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(54 제품이 사용된 논문 )

Rabbit Polyclonal TNF alpha antibody. Suitable for IHC-P, RIA, FuncS (Neut/Block), ICC/IF, IP, ELISA, WB and reacts with Mouse, Recombinant fragment - Mouse samples. Cited in 54 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Tnf.
2 이미지
Western blot - Anti-TNF alpha antibody (AB34674)
  • WB

Supplier Data

Western blot - Anti-TNF alpha antibody (AB34674)

All lanes:

Western blot - Anti-TNF alpha antibody (ab34674) at 1/1000 dilution

All lanes:

recombinant mouse TNF alpha, 50ng

Secondary

All lanes:

HRP rabbit secondary antibody at 1/40000 dilution

Predicted band size: 25 kDa

Observed band size: 17 kDa

false

Western blot - Anti-TNF alpha antibody (AB34674)
  • WB

CiteAb

Western blot - Anti-TNF alpha antibody (AB34674)

TNF alpha western blot using anti-TNF alpha antibody ab34674. Publication image and figure legend from Yang, Z. J., Wang, H. R., et al., 2019, Front Pharmacol, PubMed 31244660.

ab34674 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab34674 please see the product overview.

Myr regulated IκBα/NF-κB signaling independently of Nrf2. (A) Representative blots of p-IκB, IκB, p-P65, P65, IL-6, TNF-α, and GAPDH (n = 6); (B) calculated protein expression levels. *p < 0.05 vs. the Con group (n = 6), #p < 0.05 vs. the STZ group

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB, FuncS (Neut/Block), RIA, ELISA, IHC-P, ICC/IF, IP

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Tnf.

P06804

Specificity

<p>TNF alpha Antibody has been heated to 56°C for 30 minutes. The antibody is directed against mature 17,000 MW mouse TNF alpha and is useful in determining its presence in various assays. The antibody does not recognize mouse TNFß (lymphotoxin).</p>

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "RIA" : {"fullname" : "Radioimmunoassay", "shortname":"RIA"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "RIA-species-checked": "guaranteed", "RIA-species-dilution-info": "", "RIA-species-notes": "<p></p>", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p>It is recommended to incubate the sample with the dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Recombinant fragment - Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "RIA-species-checked": "notRecommended", "RIA-species-dilution-info": "", "RIA-species-notes": "", "FuncSNeutBlock-species-checked": "notRecommended", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

특성 및 보관 정보

제형
Liquid
Purity
IgG fraction
Purification 테크닉
Affinity purification Protein A/G
Purification 관련 사항
TNF alpha Antibody has been heated to 56°C for 30 minutes.
보관 버퍼
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity). Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (PubMed : 25586176). Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity). Promotes osteoclastogenesis and therefore mediates bone resorption (PubMed : 32741026).. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
See full target information Tnf

대체 명칭 보기

Tnfa, Tnfsf2, Tnf, Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

제품이 사용된 논문 (54)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of cell communication and signaling 19:e70048 PubMed41040887

2025

Targeting the PI3K/Akt/NF-κB axis: Cluster of differentiation 5-like-mediated immunometabolic regulation of macrophage polarization in abdominal aortic aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Hemoren Yi,Nan Liu,Zhengyang Wu,Lei Li,Tingting Li,Qixiang Liu,Man Duan,Taihu Wan

Science advances 11:eadx4183 PubMed40644532

2025

Wearing bacteriophages individually with an adhesive drug-loadable nanohelmet for treating ocular infections.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Meng,Fengmin Yang,Zhenping Cao,Chuhan Wang,Ju Chen,Yufeng Zhu,Kexin Wang,Jinyao Liu,Deyue Yan,Yan Pang

Molecular medicine (Cambridge, Mass.) 31:88 PubMed40050708

2025

Empagliflozin ameliorates renal and metabolic derangements in obese type 2 diabetic mice by blocking advanced glycation end product-receptor axis.

Applications

Unspecified application

Species

Unspecified reactive species

Takanori Matsui,Ami Sotokawauchi,Yuri Nishino,Yoshinori Koga,Sho-Ichi Yamagishi

International journal of medical sciences 21:2837-2850 PubMed39512686

2024

Integrating Network Pharmacology, Molecular Docking and Experimental Validation to Explore the Pharmacological Mechanisms of Quercetin Against Diabetic Wound.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Zhang,Lei Wang,Xuan Li,Yuxi Miao,Dongyu Li

Arkhiv patologii 86:31-37 PubMed39073539

2024

[Molecular markers of M. tuberculosis virulence in lung tissue (experimental study)].

Applications

Unspecified application

Species

Unspecified reactive species

Yu S Krylova,M A Dokhov,A S Panfilova,T I Vinogradova,I V Mokrousov,I M Kvetnoy

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2300521 PubMed37150865

2023

Selective NLRP3 Inflammasome Inhibitor MCC950 Suppresses Inflammation and Facilitates Healing in Vascular Materials.

Applications

Unspecified application

Species

Unspecified reactive species

Angus J Grant,Nianji Yang,Matthew J Moore,Yuen Ting Lam,Praveesuda L Michael,Alex H P Chan,Miguel Santos,Jelena Rnjak-Kovacina,Richard P Tan,Steven G Wise

International journal of molecular sciences 24: PubMed37175685

2023

Polyphenols and Triterpenes Combination in an In Vitro Model of Cardiac Damage: Protective Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia de Las Heras,Adrián Galiana,Sandra Ballesteros,José Carlos Quintela,Ileana Bonilauri,Vicente Lahera,Beatriz Martín-Fernández

Biomaterials advances 139:213009 PubMed35891603

2022

Peptide-siRNA nanoparticles targeting NF-κB p50 mitigate experimental abdominal aortic aneurysm progression and rupture.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Yan,Ying Hu,Antonina Akk,Samuel A Wickline,Hua Pan,Christine T N Pham

Drug design, development and therapy 16:1931-1945 PubMed35762015

2022

Dietary Supplement of . Polysaccharides Ameliorates Cognitive Dysfunction Induced by High Fat Diet via "Gut-Brain" Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Liya Fu,Wanlong Zhu,Dongmei Tian,Yong Tang,Yun Ye,Qiming Wei,Chengbin Zhang,Wenqiao Qiu,Dalian Qin,Xuping Yang,Yilan Huang

Microscopy research and technique 84:2434-2450 PubMed33908126

2021

Histological, immunohistochemical, and molecular investigation on the hepatotoxic effect of potassium dichromate and the ameliorating role of Persea americana mill pulp extract.

Applications

Unspecified application

Species

Unspecified reactive species

Basma Emad Aboulhoda,Shimaa Saad El-Din,Mohamed Mansour Khalifa,Sherif Fahmy Arsanyos,Ahmed Galal Motawie,Mohamed Shahat Sedeek,Gaber Hassan Abdelfattah,Waleed Ahmed Abdelgalil
제품이 사용된 논문 모두 보기

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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