AB195810

Anti-TNFAIP8 antibody [EPR10058(3)]

RabMAb
Recombinant
각 태그의 뜻

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(6 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal TNFAIP8 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications.

대체 명칭 보기

Tumor necrosis factor alpha-induced protein 8, TNF alpha-induced protein 8, Head and neck tumor and metastasis-related protein, MDC-3.13, NF-kappa-B-inducible DED-containing protein, SCC-S2, TNF-induced protein GG2-1, NDED, TNFAIP8

3 이미지

Supplier Data

Immunoprecipitation - Anti-TNFAIP8 antibody [EPR10058(3)] (AB195810)

TNFAIP8 was immunoprecipitated from K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab195810 at 1/70 dilution (Lane 2). Western blot was performed from the immunoprecipitate using ab195810 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1 : K562 whole cell extract (Input) 10μg. Lane 2 : ab195810 IP in K562 whole cell extract. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab195810 in K562 whole cell extract.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810)

Predicted band size: 23 kDa

false

Supplier Data

Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (AB195810)

Blocking/Dilution buffer : 5% NFDM/TBST.

Based on the sequence analysis, ab195810 recognizes three isoforms with the predicted MWs of 23KDa, 22KDa and 22KDa, respectively.

All lanes:

Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810) at 1/1000 dilution

All lanes:

Human fetal spleen lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 19 kDa,21 kDa

false

Supplier Data

Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (AB195810)

Blocking/Dilution buffer : 5% NFDM/TBST.

Based on the sequence analysis, ab195810 recognizes three isoforms with the predicted MWs of 23KDa, 22KDa and 22KDa, respectively.

All lanes:

Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (ab195810) at 1/1000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 23 kDa

Observed band size: 19 kDa,21 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10058(3)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/70", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Acts as a negative mediator of apoptosis and may play a role in tumor progression. Suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation.

See full target information Tumor necrosis factor alpha-induced protein 8

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of genomics 2021:3997045 PubMed34901263

2021

circHUWE1 Exerts an Oncogenic Role in Inducing DDP-Resistant NSCLC Progression Depending on the Regulation of miR-34a-5p/TNFAIP8.

Applications

Unspecified application

Species

Unspecified reactive species

Xueliang Yang,Quan Sun,Yongming Song,Wenli Li

Cancer research 82:221-234 PubMed34625422

2021

Novel Oncogenic Transcription Factor Cooperation in RB-Deficient Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Amy C Mandigo,Ayesha A Shafi,Jennifer J McCann,Wei Yuan,Talya S Laufer,Denisa Bogdan,Lewis Gallagher,Emanuela Dylgjeri,Galina Semenova,Irina A Vasilevskaya,Matthew J Schiewer,Chris M McNair,Johann S de Bono,Karen E Knudsen

Endocrine connections 10:471-483 PubMed33764889

2021

Long noncoding RNA DLEU2 drives the malignant behaviors of thyroid cancer through mediating the miR-205-5p/TNFAIP8 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiwen Yang,Yayin Huang,Bohan Dong,Yunhai Dai

Oncology letters 21:118 PubMed33376549

2020

Expression of TIPE family members in human colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mengya Zhong,Zhijian Chen,Yang Yan,Argen Bahet,Xin Cai,Huiyu Chen,Honggang Ran,Kaiyong Qu,Zhaopu Han,Guohong Zhuang,Shifeng Zhang,Yinan Wang

Journal of Cancer 11:3061-3071 PubMed32226521

2020

TNFAIP8 promotes the migration of clear cell renal cell carcinoma by regulating the EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Mengya Zhong,Maoshu Zhu,Yu Liu,Ying Lin,Lianghai Wang,Yuhan Ye,Huiyu Chen,Yan Yang,Guohong Zhuang,Jiyi Huang

Oncology letters 17:4667-4674 PubMed30944654

2019

TNFAIP8 promotes cisplatin resistance in cervical carcinoma cells by inhibiting cellular apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Suxia Wu,Weihua Li,Zhenghui Wu,Tianran Cheng,Ping Wang,Na Li,Xiaonan Liang,Mengmeng Chi,Shuman Zhang,Yuanfang Ma,Yanyun Li,Lihui Chai

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TNFAIP8 antibody [EPR10058(3)]

Immunoprecipitation - Anti-TNFAIP8 antibody [EPR10058(3)] (AB195810)

Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (AB195810)

Western blot - Anti-TNFAIP8 antibody [EPR10058(3)] (AB195810)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (6)

Product promise