AB137130

Anti-TNNC1 antibody [EPR9692(B)]

RabMAb
Recombinant
각 태그의 뜻

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(8 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal TNNC1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Transfected cell lysate - Human, Rat samples. Cited in 8 publications.

대체 명칭 보기

TNNC, TNNC1, TN-C

10 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

Immunohistochemical analysis using ab137130 showing negative staining in Normal human colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

Immunohistochemical analysis of paraffin embedded Human heart tissue labelling TNNC1 using ab137130 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labelling TNNC1 using ab137130 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

Immunohistochemical analysis using ab137130 showing negative staining in Normal human brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

Immunohistochemical analysis using ab137130 showing positive staining in human Heart tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

Immunohistochemical analysis using ab137130 showing negative staining in Normal human kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

Immunohistochemical analysis using ab137130 showing positive staining in human Skeletal muscle tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

WB

Lab

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000,000 dilution.

Not suitable for detecting endogenous TNNC1 in cell lines.

All lanes:

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (ab137130) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

SK-OV-3 (human ovarian cancer epithelial cell) whole cell lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 18 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

WB

Unknown

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

All lanes:

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (ab137130) at 1/1000 dilution

Lane 1:

Fetal heart lysate at 10 µg

Lane 2:

Human skeletal muscle lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

WB

Lab

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (AB137130)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab213204 was used as a loading control.

All lanes:

Western blot - Anti-TNNC1 antibody [EPR9692(B)] (ab137130) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with TNNC1 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 18 kDa

Observed band size: 20 kDa

false

Exposure time: 80s

관련 conjugated 항체와 다양한 조성의 항체 (1)

Carrier free

Anti-TNNC1 antibody [EPR9692(B)] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9692(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>This antibody is not suitable for detecting endogenous TNNC1 in cell lines.</p>

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components : Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments.

See full target information TNNC1

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제품이 사용된 논문 (8)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature cardiovascular research 3:987-1002 PubMed39196031

2024

Cardiac troponin I directly binds and inhibits mitochondrial ATP synthase with a noncanonical role in the post-ischemic heart.

Applications

Unspecified application

Species

Unspecified reactive species

Aly Elezaby,Amanda J Lin,Vijith Vijayan,Suman Pokhrel,Benjamin R Kraemer,Luiz R G Bechara,Isabel Larus,Junhui Sun,Valentina Baena,Zulfeqhar A Syed,Elizabeth Murphy,Brian Glancy,Nicolai P Ostberg,Bruno B Queliconi,Juliane C Campos,Julio C B Ferreira,Bereketeab Haileselassie,Daria Mochly-Rosen

Basic research in cardiology 118:20 PubMed37212935

2023

SMYD1a protects the heart from ischemic injury by regulating OPA1-mediated cristae remodeling and supercomplex formation.

Applications

Unspecified application

Species

Unspecified reactive species

Marta W Szulik,Steven Valdez,Maureen Walsh,Kathryn Davis,Ryan Bia,Emilee Horiuchi,Sean O'Very,Anil K Laxman,Linda Sandaklie-Nicolova,David R Eberhardt,Jessica R Durrant,Hanin Sheikh,Samuel Hickenlooper,Magnus Creed,Cameron Brady,Mickey Miller,Li Wang,June Garcia-Llana,Christopher Tracy,Stavros G Drakos,Katsuhiko Funai,Dipayan Chaudhuri,Sihem Boudina,Sarah Franklin

Life (Basel, Switzerland) 12: PubMed36013400

2022

Effects of Obesity and Diabesity on Ventricular Muscle Structure and Function in the Zucker Rat.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Sultan,Ernest Adeghate,Bright Starling Emerald,Muhammad A Qureshi,Saeed Tariq Minhas,Frank Christopher Howarth

Communications biology 4:703 PubMed34103648

2021

Random errors in protein synthesis activate an age-dependent program of muscle atrophy in mice.

Applications

Unspecified application

Species

Unspecified reactive species

James Moore,Rashid Akbergenov,Martina Nigri,Patricia Isnard-Petit,Amandine Grimm,Petra Seebeck,Lisa Restelli,Stephan Frank,Anne Eckert,Kader Thiam,David P Wolfer,Dimitri Shcherbakov,Erik C Böttger

The Veterinary record 185:694 PubMed31554713

2019

Stress cardiomyopathy in stranded cetaceans: a histological, histochemical and immunohistochemical study.

Applications

Unspecified application

Species

Unspecified reactive species

Nakita Câmara,Eva Sierra,Carolina Fernández-Maldonado,Antonio Espinosa de Los Monteros,Manuel Arbelo,Antonio Fernández,Pedro Herráez

Journal of visualized experiments : JoVE : PubMed31355804

2019

Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Zhao,Yawen Tang,Patrick J Ernst,Asher Kahn-Krell,Chengming Fan,Danielle Pretorius,Hanxi Zhu,Xi Lou,Lufang Zhou,Jianyi Zhang,Wuqiang Zhu

Journal of comparative physiology. B, Biochemical, 188:863-876 PubMed30039299

2018

Muscle-specific activation of calpain system in hindlimb unloading rats and hibernating Daurian ground squirrels: a comparison between artificial and natural disuse.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Chang,Tingyun Lei,Xiufeng Ma,Jie Zhang,Huiping Wang,Xingyuan Zhang,Yun-Fang Gao

Proteome science 14:16 PubMed27833457

2016

iTRAQ-based proteomic analysis of myofibrillar contents and relevant synthesis and proteolytic proteins in soleus muscle of hibernating Daurian ground squirrels ().

Applications

WB

Species

Unspecified reactive species

Hui Chang,Shan-Feng Jiang,Kai Dang,Hui-Ping Wang,Shen-Hui Xu,Yun-Fang Gao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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