AB179826

Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal

20ul selling size
RabMAb
Recombinant
각 태그의 뜻

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(6 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal TOMM22/TOM22 antibody. N-terminal. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

6 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (AB179826)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (AB179826)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TOMM22/TOM22 with ab179826 at 1/1000 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (AB179826)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (AB179826)

Intracellular flow cytometric analysis of 293T cells labeling TOMM22/TOM22 with ab179826 at 1/10 (red) or a rabbit IgG (negative) (green).

Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (AB179826)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (AB179826)

Immunofluorescent analysis of 293T cells labeling TOMM22/TOM22 with ab179826 at 1/100 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (AB179826)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TOMM22/TOM22 with ab179826 at 1/1000 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (AB179826)

Western blot analysis on immunoprecipitation pellet from Raji cell lysate using ab179826 at 1/10 dilution for IP.

All lanes:

Immunoprecipitation - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826)

Predicted band size: 15 kDa

false

Supplier Data

Western blot - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (AB179826)

All lanes:

Western blot - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (ab179826) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 15 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13505

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), ICC/IF, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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제품 세부 정보

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purity

Tissue culture supernatant

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Central receptor component of the translocase of the outer membrane of mitochondria (TOM) complex essential for the recognition and translocation of cytosolically synthesized mitochondrial preproteins (PubMed : 40080546). Together with the peripheral receptor TOMM20, functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore (PubMed : 10982837). The TOM complex associates with the ion channel VDAC2 and PINK1 kinase at depolarized mitochondria, this interaction stabilizes PINK1 at the outer mitochondrial membrane and triggers downstream mitophagy by the recruitment of the E3 ubiquitin ligase PRKN (PubMed : 40080546). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases (By similarity).

See full target information TOMM22

대체 명칭 보기

TOM22, TOMM22, Mitochondrial import receptor subunit TOM22 homolog, hTom22, 1C9-2, Translocase of outer membrane 22 kDa subunit homolog

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록and refine your search

EMBO reports 26:1835-1862 PubMed40055465

2025

MIA40 suppresses cell death induced by apoptosis-inducing factor 1.

Applications

Unspecified application

Species

Unspecified reactive species

Ben Hur Marins Mussulini,Klaudia K Maruszczak,Piotr Draczkowski,Mayra A Borrero-Landazabal,Selvaraj Ayyamperumal,Artur Wnorowski,Michal Wasilewski,Agnieszka Chacinska

Science advances 10:eadn7191 PubMed38848361

2024

Mechanism of human PINK1 activation at the TOM complex in a reconstituted system.

Applications

Unspecified application

Species

Unspecified reactive species

Olawale G Raimi,Hina Ojha,Kenneth Ehses,Verena Dederer,Sven M Lange,Cristian Polo Rivera,Tom D Deegan,Yinchen Chen,Melanie Wightman,Rachel Toth,Karim P M Labib,Sebastian Mathea,Neil Ranson,Rubén Fernández-Busnadiego,Miratul M K Muqit

Nature 620:1101-1108 PubMed37612504

2023

Structural mechanism of mitochondrial membrane remodelling by human OPA1.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander von der Malsburg,Gracie M Sapp,Kelly E Zuccaro,Alexander von Appen,Frank R Moss,Raghav Kalia,Jeremy A Bennett,Luciano A Abriata,Matteo Dal Peraro,Martin van der Laan,Adam Frost,Halil Aydin

Communications biology 4:138 PubMed33514783

2021

Cardiac-specific loss of mitoNEET expression is linked with age-related heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Takaaki Furihata,Shingo Takada,Naoya Kakutani,Satoshi Maekawa,Masaya Tsuda,Junichi Matsumoto,Wataru Mizushima,Arata Fukushima,Takashi Yokota,Nobuyuki Enzan,Shouji Matsushima,Haruka Handa,Yoshizuki Fumoto,Junko Nio-Kobayashi,Toshihiko Iwanaga,Shinya Tanaka,Hiroyuki Tsutsui,Hisataka Sabe,Shintaro Kinugawa

Journal of cellular biochemistry : PubMed30426531

2018

Investigating the role of CRIPTO-1 (TDGF-1) in glioblastoma multiforme U87 cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Faisal Alowaidi,Saeed M Hashimi,Maria Nguyen,Mallika Meshram,Naif Alqurashi,Brenton L Cavanagh,Bernadette Bellette,Saso Ivanovski,Adrian Meedenyia,Stephen A Wood

JCI insight 3: PubMed30089724

2018

Circulating mitochondria in deceased organ donors are associated with immune activation and early allograft dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Justin Pollara,R Whitney Edwards,Liwen Lin,Victoria A Bendersky,Todd V Brennan

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (AB179826)

Flow Cytometry (Intracellular) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (AB179826)

Immunocytochemistry/ Immunofluorescence - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (AB179826)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (AB179826)

Immunoprecipitation - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (AB179826)

Western blot - Anti-TOMM22/TOM22 antibody [EPR13505] - N-terminal (AB179826)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purity

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise