AB240282

Anti-TORC2 antibody [EPR20219] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(1 출판물)

Rabbit Recombinant Monoclonal TORC2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

대체 명칭 보기

TORC2, CRTC2, CREB-regulated transcription coactivator 2, Transducer of regulated cAMP response element-binding protein 2, TORC-2, Transducer of CREB protein 2

3 이미지

Supplier Data

Western blot - Anti-TORC2 antibody [EPR20219] - BSA and Azide free (AB240282)

This data was developed using ab184239, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1-2 : 3 minutes; Lane 3 : 15 seconds.

All lanes:

Western blot - Anti-TORC2 antibody [EPR20219] (<a href='/ko/products/primary-antibodies/torc2-antibody-epr20219-ab184239'>ab184239</a>) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 20 µg

Lane 2:

Human fetal kidney tissue lysate at 20 µg

Lane 3:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/10000 dilution

Predicted band size: 73 kDa

Observed band size: 78 kDa

true

Supplier Data

Western blot - Anti-TORC2 antibody [EPR20219] - BSA and Azide free (AB240282)

This data was developed using ab184239, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-3 : 10 seconds; Lanes 4-9 : 3 minutes.

All lanes:

Western blot - Anti-TORC2 antibody [EPR20219] (<a href='/ko/products/primary-antibodies/torc2-antibody-epr20219-ab184239'>ab184239</a>) at 1/1000 dilution

Lane 1:

A20 (mouse reticulum sarcoma cell line) whole cell lysate at 20 µg

Lane 2:

CTLL-2 (mouse T lymphocyte cell line) whole cell lysate at 20 µg

Lane 3:

YAC-1 (mouse Moloney murine leukemia virus induced lymphoma lymphoblast cell line) whole cell lysate at 20 µg

Lane 4:

Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 20 µg

Lane 5:

C6 (rat glial tumor cell line) whole cell lysate at 20 µg

Lane 6:

L6 (rat skeletal muscle cell line) whole cell lysate at 20 µg

Lane 7:

A-673 (human muscle Ewing's Sarcoma cell line) whole cell lysate at 20 µg

Lane 8:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 9:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 73 kDa

Observed band size: 78 kDa

true

Supplier Data

Western blot - Anti-TORC2 antibody [EPR20219] - BSA and Azide free (AB240282)

This data was developed using ab184239, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-6 : 3 minutes; Lanes 7-9 : 15 seconds.

All lanes:

Western blot - Anti-TORC2 antibody [EPR20219] (<a href='/ko/products/primary-antibodies/torc2-antibody-epr20219-ab184239'>ab184239</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse kidney lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Lane 4:

Rat brain lysate at 10 µg

Lane 5:

Rat kidney lysate at 10 µg

Lane 6:

Rat spleen lysate at 10 µg

Lane 7:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 20 µg

Lane 9:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 73 kDa

Observed band size: 78 kDa

true

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20219

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

제품 세부 정보

ab240282 is the carrier-free version of ab184239.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TORC2 also known as Target of Rapamycin Complex 2 is a multi-protein complex involved in regulating cell growth and survival. It consists of several subunits including mTOR RICTOR and mLST8 and has a molecular mass of 1-1.5 MDa. TORC2 acts as a serine/threonine kinase primarily phosphorylating Akt (Protein Kinase B) and other AGC family kinases. This complex is expressed in various tissues including liver muscle and brain where it influences multiple cellular functions.

Biological function summary

The actions of TORC2 play important roles in the regulation of lipid metabolism cytoskeletal organization and stress response. This complex contributes to cell survival and proliferation by phosphorylating specific substrates that control these processes. TORC2 specifically phosphorylates the hydrophobic motif on actor proteins like Akt which is necessary for their full activation. This activity establishes TORC2 as an important regulator within numerous cellular systems that contribute to maintaining cellular integrity and homeostasis.

Pathways

The activities of TORC2 are integral to the PI3K/Akt signaling and insulin signaling pathways. Within these pathways TORC2's phosphorylation of Akt impacts glucose metabolism protein synthesis and cell survival. It operates in parallel with TORC1 (Target of Rapamycin Complex 1) yet TORC2 responds to distinct extracellular cues highlighting its unique role. Key proteins associated with TORC2 in these pathways include PI3K upstream and Akt downstream which are essential for transmitting signals that affect cell growth and metabolic regulation.

Disruptions in TORC2 function have been associated with cancer and diabetes. The aberrant activation of TORC2 leads to unchecked cell growth and survival contributing to tumor progression in various cancer types. Additionally alterations in TORC2 activity can affect insulin signaling linking it to insulin resistance and type 2 diabetes. Proteins such as mTOR and Akt are central to these diseases with TORC2 playing a pivotal regulatory role in their pathways.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).

See full target information CRTC2

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

European journal of histochemistry : EJH 65: PubMed34587716

2021

Long noncoding RNA Meg3 mediates ferroptosis induced by oxygen and glucose deprivation combined with hyperglycemia in rat brain microvascular endothelial cells, through modulating the p53/GPX4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Chen,Yan Huang,Pingping Xia,Fan Zhang,Longyan Li,E Wang,Qulian Guo,Zhi Ye

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TORC2 antibody [EPR20219] - BSA and Azide free

Western blot - Anti-TORC2 antibody [EPR20219] - BSA and Azide free (AB240282)

Western blot - Anti-TORC2 antibody [EPR20219] - BSA and Azide free (AB240282)

Western blot - Anti-TORC2 antibody [EPR20219] - BSA and Azide free (AB240282)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise