AB76133

Anti-Torsin A/DYT1 antibody [EP2569Y]

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal Torsin A/DYT1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

2 이미지

Flow Cytometry (Intracellular) - Anti-Torsin A/DYT1 antibody [EP2569Y] (AB76133)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Torsin A/DYT1 antibody [EP2569Y] (AB76133)

Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Torsin A/DYT1 with purified ab76133 at 1/100 dilution (10μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

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Western blot - Anti-Torsin A/DYT1 antibody [EP2569Y] (AB76133)

All lanes:

Western blot - Anti-Torsin A/DYT1 antibody [EP2569Y] (ab76133) at 1/5000 dilution

Lane 1:

SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Rat brain lysate at 20 µg

Lane 4:

A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 6:

Mouse lung lysate at 20 µg

Lane 7:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 38 kDa

Observed band size: 35 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2569Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "For unpurified used at 1/150 <a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "For unpurified used at 1/150 <a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "For unpurified used at 1/150 <a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Torsin A also known as DYT1 is an endoplasmic reticulum-based AAA+ ATPase protein. It has a molecular mass of approximately 37 kDa. Torsin A is widely expressed in the central nervous system particularly in neurons. As part of its mechanical function Torsin A plays an important role in ATP binding and hydrolysis which drive conformational changes required for its activities. It is associated with maintaining proper protein folding and quality control within the endoplasmic reticulum and its activity supports membrane trafficking processes.

Biological function summary

Torsin A participates in neuronal functions that ensure cellular homeostasis. It functions within the hetero-oligomeric complexes aiding in the assembly of cellular structures such as the nuclear envelope. Through its interaction with nuclear envelope proteins Torsin A contributes to the maintenance of nuclear integrity. This function helps to regulate the movement of lipids and proteins between the endoplasmic reticulum and other parts of the cell indicating its role in intracellular transport mechanisms.

Pathways

This protein contributes to the dysbindin-containing biogenesis of lysosome-related organelles complex 1 (BLOC-1) pathway important in cellular trafficking processes. Torsin A interacts with other AAA+ ATPases and is closely related to LULL1 (luminal domain-like LAP1) which is a partner protein involved in similar pathways. Its function connects to cholesterol biosynthesis pathways indicating its influence on lipid homeostasis in neurons.

Mutations in the TOR1A gene that encodes Torsin A cause early-onset torsion dystonia a movement disorder affecting muscle contractions and control. Similar to DYT1 dystonia alterations in Torsin A function have links to Parkinson's disease as both conditions involve disruptions in cellular signaling pathways in neurons. Torsin A has connections to other proteins implicated in these disorders like Parkin which participates in cellular quality control impacting the same neuronal pathways disrupted in these disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Protein with chaperone functions important for the control of protein folding, processing, stability and localization as well as for the reduction of misfolded protein aggregates. Involved in the regulation of synaptic vesicle recycling, controls STON2 protein stability in collaboration with the COP9 signalosome complex (CSN). In the nucleus, may link the cytoskeleton with the nuclear envelope, this mechanism seems to be crucial for the control of nuclear polarity, cell movement and, specifically in neurons, nuclear envelope integrity. Participates in the cellular trafficking and may regulate the subcellular location of multipass membrane proteins such as the dopamine transporter SLC6A3, leading to the modulation of dopamine neurotransmission. In the endoplasmic reticulum, plays a role in the quality control of protein folding by increasing clearance of misfolded proteins such as SGCE variants or holding them in an intermediate state for proper refolding. May have a redundant function with TOR1B in non-neural tissues.

See full target information TOR1A

대체 명칭 보기

DQ2, DYT1, TA, TORA, TOR1A, Torsin-1A, Dystonia 1 protein, Torsin ATPase-1A, Torsin family 1 member A

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cellular and molecular neurobiology 44:17 PubMed38285192

2024

Treadmill Running Regulates Adult Neurogenesis, Spatial and Non-spatial Learning, Parvalbumin Neuron Activity by ErbB4 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yandong Yi,Yuejin Zhang,Yuanlong Song,Yisheng Lu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Torsin A/DYT1 antibody [EP2569Y]

Flow Cytometry (Intracellular) - Anti-Torsin A/DYT1 antibody [EP2569Y] (AB76133)

Western blot - Anti-Torsin A/DYT1 antibody [EP2569Y] (AB76133)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise