AB230263

Anti-TPK1 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal TPK1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TPK1.

대체 명칭 보기

Thiamine pyrophosphokinase 1, hTPK1, Placental protein 20, Thiamin pyrophosphokinase 1, PP20, TPK1

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPK1 antibody (AB230263)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPK1 antibody (AB230263)

Paraffin-embedded human heart tissue stained for TPK1 using ab230263 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPK1 antibody (AB230263)

Paraffin-embedded human kidney tissue stained for TPK1 using ab230263 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-TPK1 antibody (AB230263)

All lanes:

Western blot - Anti-TPK1 antibody (ab230263) at 1/1000 dilution

Lane 1:

U251 whole cell lysate at 30 µg

Lane 2:

THP-1 whole cell lysate at 30 µg

Lane 3:

MCF-7 whole cell lysate at 30 µg

Lane 4:

K562 whole cell lysate at 30 µg

Lane 5:

CT-26 whole cell lysate at 30 µg

Lane 6:

PC-3 whole cell lysate at 30 µg

Lane 7:

JK whole cell lysate at 30 µg

Lane 8:

Mouse Liver tissue lysate at 30 µg

Lane 9:

Mouse Kidney tissue lysate at 30 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/40000 dilution

Observed band size: 27 kDa

false

Exposure time: 180s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TPK1.

Q9H3S4

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TPK1 also known as thiamin pyrophosphokinase 1 is an enzyme responsible for the conversion of thiamine (vitamin B1) to thiamine pyrophosphate (TPP). The enzyme facilitates the transfer of pyrophosphate from ATP to thiamine which is the active form needed for cellular metabolism. TPK1 has a molecular mass of approximately 24 kDa. This enzyme is expressed in various tissues including liver kidney and brain reflecting its significant metabolic role.

Biological function summary

TPK1 plays an important role in energy metabolism. Thiamine pyrophosphate the product of TPK1 activity serves as an essential cofactor in several enzyme complexes including pyruvate dehydrogenase complex and alpha-ketoglutarate dehydrogenase complex. These complexes are involved in key metabolic pathways such as glycolysis and the citric acid cycle enabling the cell to produce energy efficiently. Therefore TPK1 activity supports energy production and cellular respiration.

Pathways

TPK1 significantly impacts carbohydrate metabolism and the Krebs cycle. It is involved in the thiamine metabolism pathway which is central to processing carbohydrates and generating energy. Proteins like pyruvate dehydrogenase and transketolase work in conjunction with TPK1 by using the TPP produced. These interactions highlight TPK1's role in linking thiamine conversion and energy-related pathways.

TPK1 mutations can lead to thiamine metabolism dysfunction syndrome type 5 (THMD5) and contribute to Leigh syndrome a severe neurological disorder. TPK1 dysfunction impacts brain metabolism due to its high energy demands. The disorder shares a connection with pyruvate dehydrogenase as both proteins require TPP as a cofactor. Mutations that affect TPK1 can impair these pathways leading to neurological and metabolic complications.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate (TPP) utilizing UTP and therefore links the biosynthesis of TPP to pyrimidines metabolism (PubMed : 38547260). By producing thiamine pyrophosphate, a cofactor of the mitochondrial pyruvate dehydrogenase indirectly regulates pyruvate oxidation and lipogenesis (PubMed : 38547260). Although it can also catalyze thiamine phosphorylation using ATP and CTP in vitro, it does so with significantly lower efficiency and without physiological relevance evidence (PubMed : 11342111, PubMed : 38547260).

See full target information TPK1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of clinical investigation 135: PubMed41031878

2025

Targeting specific kinase substrates rescues increased colitis severity induced by the Crohn's disease-linked LRRK2-N2081D variant.

Applications

Unspecified application

Species

Unspecified reactive species

George R Heaton,Xingjian Li,Xianting Li,Xiaoting Zhou,Yuanxi Zhang,Duc Tung Vu,Marc Oeller,Ozge Karayel,Quyen Q Hoang,Meltem Ece Kars,Nitika Kamath,Minghui Wang,Leonid Tarassishin,Matthias Mann,Inga Peter,Zhenyu Yue

Neuroreport 35:1000-1009 PubMed39190417

2024

Low expression of thiamine pyrophosphokinase-1 contributes to brain susceptibility to thiamine deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Yingfeng Xia,Ting Qian,Guoqiang Fei,Xiaoqin Cheng,Lei Zhao,Shaoming Sang,Chunjiu Zhong

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TPK1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPK1 antibody (AB230263)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPK1 antibody (AB230263)

Western blot - Anti-TPK1 antibody (AB230263)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise