AB71985

Anti-TR150 antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal TR150 antibody. Suitable for WB, IHC-P and reacts with Human, Chimpanzee samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human THRAP3 aa 900 to C-terminus.

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TR150 antibody (AB71985)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TR150 antibody (AB71985)

Paraffin embedded human colon tissue immunostained with ab71985 at 10 μg/ml.

Unknown

Western blot - Anti-TR150 antibody (AB71985)

All lanes:

Western blot - Anti-TR150 antibody (ab71985) at 0.1 µg/mL

Lane 1:

HeLa cell lysate

Lane 2:

HeLa cell lysate with immunising peptide

Predicted band size: 109 kDa

Observed band size: 109 kDa,140 kDa,40 kDa,60 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Chimpanzee

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human THRAP3 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y2W1

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TR150 also known as Transcription Regulator 150 is a protein with a molecular mass of approximately 150 kDa. You can find its expression in various tissues with higher levels in liver and brain tissues. Mechanically TR150 acts as a transcriptional regulator and binds directly to DNA to modulate the expression of different genes. Its capability to bind specific DNA sequences allows it to influence gene transcription effectively.

Biological function summary

TR150 plays a critical role in regulating gene expression that affects cellular functions like cell cycle progression and cell differentiation. It functions as part of a transcriptional regulatory complex interacting with cofactors and other transcriptional regulators to fine-tune gene expression. Its activity impacts cellular growth and development indicating its integral role in maintaining cellular homeostasis.

Pathways

TR150 is involved in the cell cycle regulation pathway and the Wnt signaling pathway. In the cell cycle pathway TR150 works alongside proteins such as Cyclin D1 to control cell division. In the Wnt signaling pathway it interacts with β-catenin and influences the transcriptional activation of Wnt target genes. Through these pathways TR150 helps in the coordination of proliferative signals and gene expression patterns.

Altered expression of TR150 has been linked to cancer and neurodegenerative diseases. Overexpression or dysfunction in TR150 can disrupt normal cell cycle control contributing to oncogenesis. In neurodegenerative diseases abnormal TR150 expression may interfere with neuronal function and survival. In these contexts it connects to proteins like p53 in cancer pathways and Tau in neurodegenerative pathways highlighting its involvement in disease mechanisms.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Involved in pre-mRNA splicing. Remains associated with spliced mRNA after splicing which probably involves interactions with the exon junction complex (EJC). Can trigger mRNA decay which seems to be independent of nonsense-mediated decay involving premature stop codons (PTC) recognition. May be involved in nuclear mRNA decay. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45 is proposed to sequester phosphorylated SFPQ from PTPRC/CD45 pre-mRNA in resting T-cells. Involved in cyclin-D1/CCND1 mRNA stability probably by acting as component of the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. Involved in response to DNA damage. Is excluced from DNA damage sites in a manner that parallels transcription inhibition; the function may involve the SNARP complex. Initially thought to play a role in transcriptional coactivation through its association with the TRAP complex; however, it is not regarded as a stable Mediator complex subunit. Cooperatively with HELZ2, enhances the transcriptional activation mediated by PPARG, maybe through the stabilization of the PPARG binding to DNA in presence of ligand. May play a role in the terminal stage of adipocyte differentiation. Plays a role in the positive regulation of the circadian clock. Acts as a coactivator of the CLOCK-BMAL1 heterodimer and promotes its transcriptional activator activity and binding to circadian target genes (PubMed : 24043798).

See full target information THRAP3

대체 명칭 보기

BCLAF2, TRAP150, THRAP3, Thyroid hormone receptor-associated protein 3, BCLAF1 and THRAP3 family member 2, Thyroid hormone receptor-associated protein complex 150 kDa component, Trap150

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell cycle (Georgetown, Tex.) 22:986-1004 PubMed36762777

2023

Metformin attenuates multiple myeloma cell proliferation and encourages apoptosis by suppressing METTL3-mediated m6A methylation of THRAP3, RBM25, and USP4.

Applications

Unspecified application

Species

Unspecified reactive species

Cong-Jie Chen,Jie-Yun Huang,Jian-Qing Huang,Jia-Yi Deng,Xiao-Hui Shangguan,Ai-Zhen Chen,Long-Tian Chen,Wei-Hao Wu

Proceedings of the National Academy of Sciences of the United States of America 110:16021-6 PubMed24043798

2013

A positive feedback loop links circadian clock factor CLOCK-BMAL1 to the basic transcriptional machinery.

Applications

IP, IP

Species

Mouse, Human

Laura Lande-Diner,Cyril Boyault,Jin Young Kim,Charles J Weitz

Molecular cell 40:126-37 PubMed20932480

2010

Phosphorylation-dependent regulation of PSF by GSK3 controls CD45 alternative splicing.

Applications

Species

Human

Florian Heyd,Kristen W Lynch

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TR150 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TR150 antibody (AB71985)

Western blot - Anti-TR150 antibody (AB71985)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise