Anti-TRA-1-60 (R) antibody [TRA-1-60]

• ICC

AbReview15359****

Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (AB16288)

Immunocytochemical analysis of ab16288 staining TRA-160 (R) in HUES7 cells. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocked in 10% serum for 1 hour at 24°C. Samples were incubated with primary antibody, dilution 1 : 500 (1% serum, 0.1% Triton in PBS), for 1 hour at 37°C. An Alexa Fluor^® 647-conjugated goat polyclonal to mouse IgM, dilution 1 : 100, was used as secondary antibody.

This image is courtesy of an Abreview submitted by Ms Fiona Lewis

• ICC

Collaborator

Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (AB16288)

This image shows a colony of Human Embryonic Stem Cells stained with dapi (blue), anti-Oct4 antibody ab19857 (red) and Tra-1-60 antibody ab16288 (green). The nuclei of Oct4-positive undifferentiated hESCs stained bright red, whereas Tra-1-60 staining was seen at the cell membrane. These antibodies can be used as markers of undifferentiated Human Embryonic Stem Cells.

This image is courtesy of an anonymous collaborator.

• ICC

AbReview57385****

Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (AB16288)

Immunocytochemistry analysis of human iPSC cells labeling TRA-1-60 (R) with ab16288 at 1 : 500 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.5% TX100. 5% serum was used to block cells for 20 minutes at 25°C. A polyclonal goat anti-mouse Cy3 secondary antibody was used at 1 : 500 dilution.

This image is courtesy of an abreview submitted by Vladimir Milenkovic, University Hospital Regensburg, Germany

• WB

Project

Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (AB16288)

As expected, ab16288 recognizes a band in Human Embryonic Stem Cell Lysate but not Mouse Embryonic Stem Cell Lysate. The smear above the main band is due to protein glycosylation. The Western Blot profile is as expected for the TRA-1-60 monoclonal antibody.

All lanes:

Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288) at 10 µg/mL

Lane 1:

Human Embryonic Stem Cell Lysate at 20 µg

Lane 2:

Mouse Embryonic Stem Cell Lysate at 20 µg

Secondary

All lanes:

Western blot - Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (<a href='/ko/products/secondary-antibodies/goat-fab2-mouse-igm-mu-chain-hrp-ab5930'>ab5930</a>) at 1/5000 dilution

Predicted band size: 235 kDa

Observed band size: 250 kDa

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