AB72625

Anti-TRA2A antibody

Name: Anti-TRA2A antibody
Brand: Abcam Limited
SKU: AB72625
Rating: 5 (1 reviews)

(1 리뷰)

(3 제품이 사용된 논문 )

Rabbit Polyclonal TRA2A antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TRA2A.

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRA2A antibody (AB72625)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRA2A antibody (AB72625)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab72625 at 1 : 50 dilution.
Left image un-treated.
Right image treated with immunizing peptide.

Immunocytochemistry/ Immunofluorescence - Anti-TRA2A antibody (AB72625)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRA2A antibody (AB72625)

Immunofluorescence analysis of Hela cells using ab72625 at a 1 : 500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.

AbReview39331****

Western blot - Anti-TRA2A antibody (AB72625)

All lanes:

Western blot - Anti-TRA2A antibody (ab72625) at 1/250 dilution

Lanes 1, 3 and 5:

293T whole cell lysate at 5 µg

Lanes 2, 4 and 6:

Mouse ES (129Sv) whole cell lysate at 5 µg

Secondary

All lanes:

HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution

Predicted band size: 33 kDa

Observed band size: 27 kDa,33 kDa,85 kDa

false

Exposure time: 1min

This image is courtesy of an anonymous Abreview

Unknown

Western blot - Anti-TRA2A antibody (AB72625)

All lanes:

Western blot - Anti-TRA2A antibody (ab72625) at 1/500 dilution

Lane 1:

Extracts from HUVEC cells at 5 µg

Lane 2:

Extracts from HUVEC cells plus 5µg immunizing peptide at 5 µg

Predicted band size: 33 kDa

Observed band size: 27 kDa,33 kDa,44 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human TRA2A. The exact immunogen used to generate this antibody is proprietary information.

Q13595

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TRA2A also known as transformer 2 alpha homolog is an RNA-binding protein involved in numerous cellular processes. This protein has a molecular weight of approximately 31 kDa. It is expressed in many tissues including the brain and testes. TRA2A binds to specific RNA sequences modulating splicing events and affecting the choice of splice sites. It belongs to the serine/arginine-rich protein family which plays key roles in the regulation of both alternative and constitutive splicing.

Biological function summary

TRA2A influences gene expression and transcript variants by engaging in complex interactions. It acts as part of the spliceosome machinery which is a large ribonucleoprotein complex responsible for splicing pre-mRNA. TRA2A ensures precise removal of introns and ligation of exons producing different mRNA isoforms. These isoforms translate into functionally distinct proteins influencing cellular activity.

Pathways

TRA2A participates in the mRNA processing pathway influencing genetic output by regulating splice site selection. TRA2A connects to the MAPK signaling pathway as it is known to interact with proteins like SAM68 which also affects mRNA splicing. This connectivity with other proteins highlights the regulatory network's complexity ensuring accurate gene expression profiles.

TRA2A has been implicated in cancer and neurodegenerative disorders. Altered expression and splicing activity of TRA2A contribute to oncogenesis as seen in breast cancer where abnormal splicing can drive tumor progression. TRA2A also impacts neurological conditions interplaying with proteins like tau in Alzheimer's disease where aberrant splicing plays a part in disease pathology. Understanding these links offers potential therapeutic angles for targeting TRA2A-related pathways.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.

See full target information TRA2A

대체 명칭 보기

Transformer-2 protein homolog alpha, TRA-2 alpha, TRA2-alpha, Transformer-2 protein homolog A, TRA2A

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of Cancer 12:4883-4890 PubMed34234858

2021

TRA2A Binds With LncRNA MALAT1 To Promote Esophageal Cancer Progression By Regulating EZH2/β-catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Zhao,Qiuyang Chen,Yujie Cai,Danze Chen,Mingrong Bei,Hongyan Dong,Jianzhen Xu

Cell reports 25:3422-3434.e7 PubMed30566867

2018

An Integrative Study of Protein-RNA Condensates Identifies Scaffolding RNAs and Reveals Players in Fragile X-Associated Tremor/Ataxia Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando Cid-Samper,Mariona Gelabert-Baldrich,Benjamin Lang,Nieves Lorenzo-Gotor,Riccardo Delli Ponti,Lies-Anne W F M Severijnen,Benedetta Bolognesi,Ellen Gelpi,Renate K Hukema,Teresa Botta-Orfila,Gian Gaetano Tartaglia

Life science alliance 1:e201800070 PubMed30456350

2018

Proteomics and neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Hannelore Hartmann,Daniel Hornburg,Mareike Czuppa,Jakob Bader,Meike Michaelsen,Daniel Farny,Thomas Arzberger,Matthias Mann,Felix Meissner,Dieter Edbauer

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRA2A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRA2A antibody (AB72625)

Immunocytochemistry/ Immunofluorescence - Anti-TRA2A antibody (AB72625)

Western blot - Anti-TRA2A antibody (AB72625)

Western blot - Anti-TRA2A antibody (AB72625)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise