Anti-TRADD antibody [EPR3604] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-TRADD antibody [EPR3604] - BSA and Azide free (AB238960)

ab110644, at 1/100, staining TRADD in HeLa cells by Immunofluorescence.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab110644).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody [EPR3604] - BSA and Azide free (AB238960)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling TRADD with purified ab110644 at 1/850 dilution (0.7 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab110644)

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-TRADD antibody [EPR3604] - BSA and Azide free (AB238960)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling TRADD with purified ab110644 at 1/600 dilution (1 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab110644)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody [EPR3604] - BSA and Azide free (AB238960)

ab110644, at 1/100, staining TRADD in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing Tris glycine, Tissue culture supernatant, BSA, glycerol and sodium azide (ab110644).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRADD antibody [EPR3604] - BSA and Azide free (AB238960)

Immunocytochemistry/ Immunofluorescence analysis of MCF7(Human breast adenocarcinoma epithelial cell) cells labeling TRADD with purified ab110644 at 1/100 dilution (6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab110644)