Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free (AB323703)

This data was developed using ab323702, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / 293T (human embryonic kidney epithelial cell) cells transfected with a mouse TRAIL expression vector containing a Myc tag (Middle) /293T cells transfected with an empty expression vector containing a Myc tag (Right) cells labelling TRAIL with ab323702 at 1/5000 dilution (0.01 ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were surface stained with isotype control (ab172730) or ab323702. Then fixed with 2% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor®647.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free (AB323703)

This data was developed using ab323702, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse spleen cell cells labelling TRAIL with ab323702 at 1/50 dilution (1 ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Mouse spleen cells were co-stained with anti mouse CD19 conjugated to PE/Cy7.
Gated on viable cell.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free (AB323703)

This data was developed using ab323702, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse spleen cell cells labelling TRAIL with ab323702 at 1/50 dilution (1 ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Mouse spleen cells were co-stained with anti mouse CD11b conjugated to BV421.
Gated on viable cell.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free (AB323703)

This data was developed using ab323702, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse spleen cell cells labelling TRAIL with ab323702 at 1/50 dilution (1 ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Mouse spleen cells were co-stained with anti mouse CD3 conjugated to Alexa Fluor®647.
Gated on viable cell.

• WB

Supplier Data

Western blot - Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free (AB323703)

This data was developed using ab323702, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of TRAIL is induced by LPS treatment (PMID : 9603453).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TRAIL antibody [EPR28587-33] (<a href='/ko/products/primary-antibodies/trail-antibody-epr28587-33-ab323702'>ab323702</a>) at 1/1000 dilution

Lane 1:

Untreated mouse lung tissue lysate at 40 µg

Lane 2:

Mouse lung treated with 1ug/ml LPS and 1ug/ml BFA for 16h whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 28-30 kDa,36 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-TRAIL antibody [EPR28587-33] - BSA and Azide free (AB323703)

This data was developed using ab323702, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of TRAIL is induced by LPS treatment (PMID : 9603453).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TRAIL antibody [EPR28587-33] (<a href='/ko/products/primary-antibodies/trail-antibody-epr28587-33-ab323702'>ab323702</a>) at 1/1000 dilution

Lane 1:

Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 40 µg

Lane 2:

RAW 264.7 treated with 100 ng/ml LPS for 6h and 300ng/ml BFA for 3h whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 28-30 kDa,36 kDa

false

Exposure time: 26s