AB109503

Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control

Name: Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control
Brand: Abcam Limited
SKU: AB109503
Rating: 5 (2 reviews)

20ul selling size
RabMAb
Recombinant
Lab Essentials
각 태그의 뜻

(2 리뷰들)

(11 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Transferrin antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 11 publications.

3 이미지

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (AB109503)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labelling Transferrin with ab109503 at 1/50 dilution (1μg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Unknown

Western blot - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (AB109503)

All lanes:

Western blot - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503) at 1/1000 dilution

Lane 1:

fetal liver lysate at 10 µg

Lane 2:

prostate cancer lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 77 kDa

false

Lab

Western blot - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (AB109503)

All lanes:

Western blot - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (ab109503) at 1/10000 dilution

All lanes:

Human plasma lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 77 kDa

Observed band size: 77 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2932(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Transferrin also known as serotransferrin or siderophilin is a glycoprotein with a mass of approximately 80 kDa. It is primarily synthesized in the liver and subsequently secreted into the bloodstream. Transferrin has an important role in iron transport and maintains iron homeostasis by binding and delivering iron to various tissues throughout the body. It can bind two ferric ions (Fe3+) in association with an anion usually bicarbonate. In biological fluids transferrin exists in serum plasma and other extracellular fluids.

Biological function summary

Transferrin facilitates the transportation of iron ions. It delivers iron to cells by binding to transferrin receptors on cell surfaces forming a complex that gets internalized via receptor-mediated endocytosis. Inside the endosomes acidic conditions cause iron to release from transferrin enabling its utilization in cellular processes like DNA synthesis and electron transport. Transferrin itself acts independently and does not form part of a larger protein complex. Variants of transferrin include mouse transferrin bovine transferrin and biotinylated transferrin each with similar function across different species.

Pathways

Transferrin operates centrally in iron metabolism and homeostasis pathways. It functions in coordination with the transferrin receptor 1 (TfR1) which facilitates cellular uptake of the transferrin-iron complex. Additionally transferrin plays a role in the hepcidin regulatory pathway. Hepcidin modulates iron homeostasis by decreasing iron absorption in the intestine and controlling iron release from macrophages and hepatocytes. Transferrin's ability to bind iron connects it to other iron-containing proteins such as ferritin which stores excess iron in cells.

Several iron-related conditions can impact transferrin function including anemia and hemochromatosis. Anemia often occurs when there is insufficient iron delivery leading to inadequate hemoglobin synthesis and reduced oxygen transport. Aberrant transferrin receptor activity affects iron uptake in such conditions. Hemochromatosis characterized by iron overload can occur due to mutations in genes like HFE leading to changes in hepcidin regulation and increased intestinal iron absorption. Transferrin levels and saturation are clinical indicators used to assess iron status in such diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.. (Microbial infection) Serves as an iron source for Neisseria species, which capture the protein and extract its iron for their own use.. (Microbial infection) Serves as an iron source for parasite T.brucei (strain 427), which capture TF via its own transferrin receptor ESAG6 : ESAG7 and extract its iron for its own use.

See full target information TF

대체 명칭 보기

PRO1400, TF, Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin

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제품이 사용된 논문 (11)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology letters 28:298 PubMed38751752

2024

Mechanism of targeting the mTOR pathway to regulate ferroptosis in NSCLC with different EGFR mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Chunjiao Wu,Rui Zhong,Tianxue Wei,Yulong Jin,Chunying He,Hui Li,Ying Cheng

Oxidative medicine and cellular longevity 2023:9808100 PubMed36654781

2023

TRIM6 Reduces Ferroptosis and Chemosensitivity by Targeting SLC1A5 in Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Ping Dong,Nian Liu,Jun-Yuan Yang,Hui-Min Wang,Qing Geng

Frontiers in cardiovascular medicine 9:1003282 PubMed36172581

2022

Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wanrong Man,Xinglong Song,Zhenyu Xiong,Jing Gu,Jie Lin,Xiaoming Gu,Duan Yu,Congye Li,Mengyuan Jiang,Xuebin Zhang,Zhi Yang,Yang Cao,Yan Zhang,Xiaofei Shu,Dexi Wu,Haichang Wang,Gang Ji,Dongdong Sun

International journal of molecular sciences 23: PubMed35743034

2022

Large Benefit from Simple Things: High-Dose Vitamin A Improves -Related Retinal Dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Vasily M Smirnov,Baptiste Wilmet,Marco Nassisi,Christel Condroyer,Aline Antonio,Camille Andrieu,Céline Devisme,Serge Sancho,José-Alain Sahel,Christina Zeitz,Isabelle Audo

Cell death and differentiation 29:1513-1527 PubMed35105963

2022

EBV infection-induced GPX4 promotes chemoresistance and tumor progression in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yuan,Shibing Li,Qiuyan Chen,Tianliang Xia,Donghua Luo,Liangji Li,Sailan Liu,Shanshan Guo,Liting Liu,Chaochao Du,Guodong Jia,Xiaoyun Li,Zijian Lu,Zhenchong Yang,Huanliang Liu,Haiqiang Mai,Linquan Tang

Molecular medicine reports 24: PubMed34165161

2021

Downregulation of VDR in benign paroxysmal positional vertigo patients inhibits otolith‑associated protein expression levels.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Zhang,Juanli Xing,Yulin Gong,Ping Li,Boqian Wang,Ling Xu

Clinical and translational medicine 11:e390 PubMed33931967

2021

Deubiquitinase USP35 modulates ferroptosis in lung cancer via targeting ferroportin.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Tang,Wanli Jiang,Ming Mao,Jinping Zhao,Jiakuan Chen,Nitao Cheng

Theranostics 11:5650-5674 PubMed33897873

2021

Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zhang,Keke Yu,Lifang Ma,Zijun Qian,Xiaoting Tian,Yayou Miao,Yongjie Niu,Xin Xu,Susu Guo,Yueyue Yang,Zhixian Wang,Xiangfei Xue,Chuanjia Gu,Wentao Fang,Jiayuan Sun,Yongchun Yu,Jiayi Wang

International journal of nanomedicine 15:6183-6200 PubMed32922001

2020

Evidence Supporting the Safety of Pegylated Diethylaminoethyl-Chitosan Polymer as a Nanovector for Gene Therapy Applications.

Applications

Unspecified application

Species

Unspecified reactive species

Elsa Patricia Rondon,Houda Abir Benabdoun,Francis Vallières,Maicon Segalla Petrônio,Marcio José Tiera,Mohamed Benderdour,Julio Cesar Fernandes

Clinical proteomics 17:20 PubMed32518534

2020

Proteomic analysis of human synovial fluid reveals potential diagnostic biomarkers for ankylosing spondylitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Hyun Lee,Jae Hun Jung,Jeesoo Kim,Won-Ki Baek,Jinseol Rhee,Tae-Hwan Kim,Sang-Hyon Kim,Kwang Pyo Kim,Chang-Nam Son,Jong-Seo Kim

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control

Flow Cytometry (Intracellular) - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (AB109503)

Western blot - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (AB109503)

Western blot - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (AB109503)

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Reagents, Controls & Accessories

Reagents, Controls & Accessories

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Assay Kits

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (11)

Product promise