Anti-Transferrin Receptor antibody [EPR4013]

• WB

Unknown

Western blot - Anti-Transferrin Receptor antibody [EPR4013] (AB109259)

All lanes:

Western blot - Anti-Transferrin Receptor antibody [EPR4013] (ab109259) at 1/10000 dilution

Lane 1:

Human placenta lysate at 10 µg

Lane 2:

MCF7 lysate at 10 µg

Lane 3:

JAR lysate at 10 µg

Predicted band size: 84 kDa

Observed band size: 90 kDa

false

• WB

Lab

Western blot - Anti-Transferrin Receptor antibody [EPR4013] (AB109259)

Anti-Transferrin Receptor antibody [EPR4013] staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab109259 was shown to bind specifically to Transferrin Receptor. A band was observed at 90 kDa in wild-type K562 cell lysates with no signal observed at this size in Tfrc knockout cell line. To generate this image, wild-type and Tfrc knockout K562 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Transferrin Receptor antibody [EPR4013] (ab109259) at 1/10000 dilution

Lane 1:

Wild-type K562 cell lysate at 20 µg

Lane 2:

Western blot - Human TFRC knockout K562 cell line (<a href='/ko/products/cell-lines/human-tfrc-knockout-k562-cell-line-ab288185'>ab288185</a>)

Lane 2:

TFRC knockout K562 cell lysate at 20 µg

Lane 3:

HDLM-2 cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Observed band size: 90 kDa

false