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AB245003

Anti-Trefoil Factor 3 antibody [EPR21130-240] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Trefoil Factor 3 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
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Sandwich ELISA - Anti-Trefoil Factor 3 antibody [EPR21130-240] - BSA and Azide free (Detector) (AB245003)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Trefoil Factor 3 antibody [EPR21130-240] - BSA and Azide free (Detector) (AB245003)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab233617).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21130-240

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB233617

Human TFF3 ELISA Kit

Recombinant|SimpleStep

ELISA - Human TFF3 ELISA Kit (AB233617)

  • 0
  • 0
Sample Prep & Detection Kits

AB201802

DyLight® 633 Conjugation Kit (Fast) - Lightning-Link®

Schematic Diagram - DyLight® 633 Conjugation Kit (Fast) - Lightning-Link® (AB201802)

  • 0
  • 0
Sample Prep & Detection Kits

AB269902

Gold-Maleimide Conjugation Kit (20nm, 20 OD)

Lateral flow assay - Gold-Maleimide Conjugation Kit (20nm, 20 OD) (AB269902)

  • 0
  • 0
Primary Antibodies

AB285103

Anti-Clusterin antibody [EPR22533-240] - BSA and Azide free (Detector)

RabMAb|Recombinant

  • 0
  • 0
Primary Antibodies

AB272692

Anti-MUC2 antibody [EPR23479-47]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|Trial Size

Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (AB272692)

  • 5
  • 7
Primary Antibodies

AB276150

Anti-TFF2 antibody [EPR23231-30] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-TFF2 antibody [EPR23231-30] - BSA and Azide free (Detector) (AB276150)

  • 0
  • 0
Primary Antibodies

AB244826

Anti-Anterior Gradient 2 antibody [EPR20164-54] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Anterior Gradient 2 antibody [EPR20164-54] - BSA and Azide free (Capture) (AB244826)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab245003 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human TFF3. The recommended pair for sandwich ELISA is:
Capture: ab244735, Human TFF3 Capture Antibody (unconjugated)
Detector: ab245003, Human TFF3 Detector Antibody (unconjugated)
The reference range value is 3.1 - 200 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Trefoil Factor 3 (TFF3) also known as intestinal trefoil factor is a small peptide with a molecular mass around 7 kDa. This protein is characterized by the presence of a trefoil domain which consists of a stable compact structure important for its function. TFF3 has significant expression in the gastrointestinal tract notably in the colon and small intestine where it acts to maintain the integrity of the mucosal barrier. It can also be found in other tissues but its concentrations vary depending on factors like tissue type and health status.
Biological function summary

TFF3 plays an important role in protecting and repairing epithelial tissues particularly those lining the gastrointestinal tract. This protein promotes epithelial restitution by aiding cell migration and inhibits apoptosis contributing to tissue healing processes. While TFF3 does not form part of a large protein complex it often interacts with mucins specifically mucin 2 to bolster the protective mucus layer which is essential for shielding epithelial cells.

Pathways

Trefoil Factor 3 is notably involved in pathways associated with mucosal immune response and inflammation. A significant pathway involving TFF3 is the mucosal defense pathway where it works alongside other mucins and proteins like epidermal growth factor (EGF) to mediate regeneration and defense of epithelial tissues after injury. It also engages in the Wnt signaling pathway where it affects cell proliferation and migration critical for maintaining epithelial homeostasis.

TFF3 has links to inflammatory bowel disease (IBD) and colorectal cancer. In IBD its expression levels may be altered affecting the ability of the intestine to protect itself and heal contributing to the disease's progression. In colorectal cancer TFF3 can exhibit upregulated expression linking it to tumor growth and metastasis. Proteins such as beta-catenin in the Wnt signaling pathway which are associated with cell cycle regulation have connections with TFF3 highlighting its role in cancerous transformations.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
See full target information TFF3

대체 명칭 보기

ITF, TFI, TFF3, Trefoil factor 3, Intestinal trefoil factor, Polypeptide P1.B, hITF, hP1.B
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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