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AB67800

Anti-TRFP/MED20 antibody

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Mouse Polyclonal TRFP/MED20 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MED20.
1 이미지
Immunocytochemistry/ Immunofluorescence - Anti-TRFP/MED20 antibody (AB67800)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRFP/MED20 antibody (AB67800)

ab67800 at 10μg/ml staining TRFP/MED20 in Hela cells.

주요 정보

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MED20.

Q9H944

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보완 제품

Primary Antibodies

AB109186

Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker

BOND RX™ Validated|20ul selling size|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Olig2 antibody [EPR2673] - Oligodendrocyte Marker (AB109186)

  • 5
  • 22
Primary Antibodies

AB4680

Anti-Neurofilament heavy polypeptide antibody

Immunohistochemistry - Free Floating - Anti-Neurofilament heavy polypeptide antibody (AB4680)

  • 5
  • 13
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 76
Primary Antibodies

AB251760

Anti-VPS37A/HCRP1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-VPS37A/HCRP1 antibody (AB251760)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TRFP also known as MED20 is part of the Mediator complex which acts as a communication bridge between gene-specific regulatory proteins and RNA polymerase II. MED20 has a molecular mass of approximately 22 kDa and is essential for the transcriptional coactivation process. This protein is highly expressed in various human tissues including the liver heart and kidneys with detectable levels in several other organs. MED20 facilitates the activation of transcription by interacting with multiple transcription factors.
Biological function summary

The Mediator complex which includes MED20 as one of its subunits plays a significant role in the initiation of transcription. MED20 forms part of the larger Mediator complex working closely with other subunits to modulate gene expression. By serving as a scaffold it helps stabilize interactions between transcription factors and RNA polymerase II enhancing transcription efficiency. The complex influences cell growth differentiation and response to cellular signals.

Pathways

MED20's presence in the Mediator complex situates it in critical transcription regulation pathways such as the RNA polymerase II transcription initiation pathway. It interacts with proteins like CDK8 and CCNC integral in controlling transcription by regulating Mediator's cyclin-dependent kinase activity. MED20 fosters connections between regulatory proteins and RNA polymerase II affecting transcription-related cellular signaling.

Abnormalities in the Mediator complex including issues with MED20 have implications in certain disorders like cancer and cardiovascular disease. Dysregulation of gene expression through interrupted Mediator activity can lead to these conditions. Specifically deficiencies involving MED20 affect the overall integrity of the Mediator complex relating to proteins such as MED12 and MED1 which have associations with oncogenesis and developmental defects.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
See full target information MED20

대체 명칭 보기

TRFP, MED20, Mediator of RNA polymerase II transcription subunit 20, Mediator complex subunit 20, TRF-proximal protein homolog, hTRFP
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Heliyon 9:e22086 PubMed38046167

2023

Long non-coding RNA PCAT5 regulates the progression of Esophageal Squamous Cell Carcinoma via miR-4295/PHF20.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Liu,Hang Yin,Tao Yang,Jiacai Lin,Tingting Sun
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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