Anti-TRIF antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIF antibody (AB13810)

Immunohistochemistrical analysis of a FFPE tissue section of mouse spleen using 1/200 dilution of ab13810. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

• WB

Supplier Data

Western blot - Anti-TRIF antibody (AB13810)

All lanes:

Western blot - Anti-TRIF antibody (ab13810) at 1/1000 dilution

Lane 1:

Mouse spleen lysate with immunizing peptide

Lane 2:

Mouse spleen lysate

Predicted band size: 156 kDa,17 kDa,40 kDa,42 kDa,75 kDa,76 kDa

Observed band size: 13 kDa,17 kDa,20 kDa,25 kDa,35 kDa,50 kDa,75 kDa

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• WB

Supplier Data

Western blot - Anti-TRIF antibody (AB13810)

All lanes:

Western blot - Anti-TRIF antibody (ab13810) at 1/1000 dilution

Lane 1:

Mouse intestine lysate

Lane 2:

Mouse spleen lysate

Lane 3:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate

Predicted band size: 76 kDa

false

• WB

CiteAb

Western blot - Anti-TRIF antibody (AB13810)

TRIF western blot using anti-TRIF antibody ab13810. Publication image and figure legend from Lin, S., Liang, Y., et al., 2012, J Neuroinflammation, PubMed 22361049.

ab13810 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab13810 please see the product overview.

TIR-domain-containing adapter-inducing interferon-β (TRIF) is expressed by microglia, but not by astrocytes or neurons, and is expressed in a time-dependent manner. (A) Retinas were collected from sham-operated mice and from TRIF-deficient mice at 1, 3, and 7 days post-crush (1dPC, 3dPC, and 7dPC, respectively). TRIF expression was examined by western blot, which showed that in the sham-operated and 1dPC groups, TRIF expression was limited. At 3dPC and 7dPC, TRIF expression was upregulated. GAPDH and β-actin were used as internal controls. *p < 0.05, increase relative to sham control. (B) Using dual-label immunofluorescence staining, expression of TRIF was detected in microglia but not neurons and astroglia. Iba-1, βIII-tubulin and glial fibrillary acidic protein (GFAP) were used to identify microglia, neurons, and astroglia in retinal sections. Scale bar = 20 μm. Scale bar (in box) = 10 μm.

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