AB244380

Anti-TRIM29 antibody

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(4 제품이 사용된 논문 )

Rabbit Polyclonal TRIM29 antibody. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRIM29 aa 50 to C-terminus.

대체 명칭 보기

ATDC, TRIM29, Tripartite motif-containing protein 29, Ataxia telangiectasia group D-associated protein

8 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)

Paraffin-embedded human skin tissue stained for TRIM29 using ab244380 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)

Paraffin-embedded human stomach tissue stained for TRIM29 using ab244380 at 1/500 dilution in immunohistochemical analysis.

No positivity in glandular cells as expected.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)

Paraffin-embedded human prostate tissue stained for TRIM29 using ab244380 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-TRIM29 antibody (AB244380)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRIM29 antibody (AB244380)

PFA-fixed, Triton X-100 permeabilized SiHa cells stained for TRIM29 (green) using ab244380 at 4 μg/ml in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)

Paraffin-embedded human esophagus tissue stained for TRIM29 using ab244380 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)

Paraffin-embedded human placenta tissue stained for TRIM29 using ab244380 at 1/500 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-TRIM29 antibody (AB244380)

All lanes:

Western blot - Anti-TRIM29 antibody (ab244380) at 0.4 µg/mL

All lanes:

RT4 (Human urinary bladder cancer cell line) cell lysate

Predicted band size: 65 kDa

false

Unknown

Western blot - Anti-TRIM29 antibody (AB244380)

All lanes:

Western blot - Anti-TRIM29 antibody (ab244380) at 0.4 µg/mL

Lane 1:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (Rat cell line) cell lysate

Predicted band size: 65 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human TRIM29 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14134

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The tripartite motif-containing protein 29 (TRIM29) also known as ATDC is a protein with a molecular mass of approximately 66 kDa. TRIM29 is part of the TRIM family characterized by its RING finger motif B-box type 1 and 2 domains and a coiled-coil region which are significant for its involvement in ubiquitination processes. Researchers have found TRIM29 expression largely in epithelial tissues exhibiting varied levels of expression depending on tissue type.

Biological function summary

TRIM29 functions as an essential regulator of DNA damage responses and cellular proliferation. It is not found as part of any larger protein complex but interacts with various other proteins to carry out its function. TRIM29 can influence the activity of key molecules involved in DNA repair particularly through its interaction with DNA-dependent protein kinase catalytic subunit (DNA-PKcs) which enhances cell survival after genotoxic stress. It participates in ensuring proper functioning of cell cycle checkpoints and modulation of apoptosis.

Pathways

TRIM29 participates in the DNA damage response pathway and the WNT signaling pathway. It interacts with proteins such as β-catenin which influences the transcriptional regulation of target genes involved in cell proliferation. Through these interactions TRIM29 influences cellular responses to DNA damage and the regulation of cell growth linking it to critical pathways that maintain genomic stability and cell integrity.

Aberrant TRIM29 expression correlates with various cancers including breast and colorectal cancers. In breast cancer TRIM29 affects the pathways involving p53 a known tumor suppressor protein. In colorectal cancer its overexpression is associated with enhanced cancer cell proliferation and survival often in conjunction with KRAS mutations. TRIM29's regulatory role in these pathways highlights its potential as a biomarker and a therapeutic target in oncology.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays a crucial role in the regulation of macrophage activation in response to viral or bacterial infections within the respiratory tract. Mechanistically, TRIM29 interacts with IKBKG/NEMO in the lysosome where it induces its 'Lys-48' ubiquitination and subsequent degradation. In turn, the expression of type I interferons and the production of pro-inflammatory cytokines are inhibited. Additionally, induces the 'Lys-48' ubiquitination of STING1 in a similar way, leading to its degradation.

See full target information TRIM29

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PeerJ 12:e16921 PubMed38426148

2024

Identification and immunoinfiltration analysis of key genes in ulcerative colitis using WGCNA.

Applications

Unspecified application

Species

Unspecified reactive species

Siyi Ni,Yingchao Liu,Jihong Zhong,Yan Shen

Cell biology international 47:1126-1135 PubMed36841942

2023

TRIM29 promotes podocyte pyroptosis in diabetic nephropathy through the NF-kB/NLRP3 inflammasome pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Xu,Zihan Qin,Ce Zhang,Xia Mi,Chi Zhang,Feihong Zhou,Junsheng Wang,Liexiang Zhang,Fei Hua

Molecular medicine reports 22:3822-3832 PubMed32901838

2020

Long non‑coding RNA TP73‑AS1 accelerates the progression and cisplatin resistance of non‑small cell lung cancer by upregulating the expression of TRIM29 via competitively targeting microRNA‑34a‑5p.

Applications

Unspecified application

Species

Unspecified reactive species

Shunxiang Luo,Ming Shen,Hui Chen,Weiwei Li,Cong Chen

Cancer management and research 12:4981-4990 PubMed32612385

2020

miRNA-181a-5p Enhances the Sensitivity of Cells to Cisplatin in Esophageal Adenocarcinoma by Targeting CBLB.

Applications

Unspecified application

Species

Unspecified reactive species

Song Yang,Peng Wang,Songhua Wang,Aihua Cong,Qi Zhang,Wenhao Shen,Xiangyi Li,Wei Zhang,Gaohua Han

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRIM29 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)

Immunocytochemistry/ Immunofluorescence - Anti-TRIM29 antibody (AB244380)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)

Western blot - Anti-TRIM29 antibody (AB244380)

Western blot - Anti-TRIM29 antibody (AB244380)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise