AB312833

Anti-TRIM67 antibody [EPR25994-6] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal TRI67 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat, Transfected cell lysate samples.

대체 명칭 보기

TNL, TRIM67, Tripartite motif-containing protein 67, TRIM9-like protein

2 이미지

Supplier Data

Western blot - Anti-TRIM67 antibody [EPR25994-6] - BSA and Azide free (AB312833)

This data was developed using ab312832, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : lung (PMID : 31420404), liver (PMID : 31420404). The bands beneath the target band (83kDa) are likely to be degraded target fragments. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 15 seconds

All lanes:

Western blot - Anti-TRIM67 antibody [EPR25994-6] (<a href='/ko/products/primary-antibodies/trim67-antibody-epr25994-6-ab312832'>ab312832</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 60 µg

Lane 2:

Human lung tissue lysate at 60 µg

Lane 3:

human liver tissue lysate at 60 µg

Lane 4:

Mouse cerebellum tissue lysate at 60 µg

Lane 5:

Mouse lung tissue lysate at 60 µg

Lane 6:

Mouse liver tissue lysate at 60 µg

Lane 7:

Rat cerebellum tissue lysate at 20 µg

Lane 8:

Rat lung tissue lysate at 60 µg

Lane 9:

Rat liver tissue lysate at 60 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 83 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-TRIM67 antibody [EPR25994-6] - BSA and Azide free (AB312833)

This data was developed using ab312832, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 3 seconds

All lanes:

Western blot - Anti-TRIM67 antibody [EPR25994-6] (<a href='/ko/products/primary-antibodies/trim67-antibody-epr25994-6-ab312832'>ab312832</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containi a His tag whole cell lysate at 20 µg

Lane 2:

293T transfected with a TRIM67 expression vector containi a His tag whole cell lysate at 20 µg

Lane 3:

293T transfected with a TRIM9 expression vector containi a His tag whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 83 kDa

false

Exposure time: 3s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25994-6

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TRIM67 also known as Tripartite Motif Containing 67 is a protein encoded by the TRIM67 gene. It belongs to the tripartite motif (TRIM) family of proteins characterized by an N-terminal RING finger domain one or two B-box motifs and a coiled-coil region. TRIM67 has a molecular mass of approximately 53 kDa. This protein is mainly expressed in the brain particularly in neurons highlighting its importance in neural tissues.

Biological function summary

TRIM67 plays an important role in neuronal development and differentiation. It contributes to processes like axon outgrowth and guidance which are critical for proper neuronal network formation. TRIM67 does not function as part of a complex but interacts with other proteins for executing its roles. It is known to modulate several signaling pathways key to cell proliferation and survival emphasizing its importance in cellular maintenance and integrity.

Pathways

TRIM67 impacts the regulation of the Wnt signaling pathway which is essential for numerous developmental processes. Moreover it has a relationship with the PI3K/AKT signaling pathway which is important for cell growth and apoptosis. In these pathways TRIM67 can interact with proteins like DVL and AKT1 contributing to their regulation and overall pathway stability. This connectivity underlines the multifaceted role TRIM67 plays in cell signaling and developmental biology.

TRIM67 has been associated with neurological disorders given its strong expression in neuronal tissues. Specifically it relates to conditions like autism spectrum disorders (ASD) where disruptions in its expression or mutations could impact neural connectivity. Additionally neurodegenerative diseases such as Alzheimer's disease may also involve TRIM67 due to its role in neuronal survival and function. These associations highlight a connection with proteins like APP and BDNF which are linked to neural health and disease.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information TRIM67

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRIM67 antibody [EPR25994-6] - BSA and Azide free

Western blot - Anti-TRIM67 antibody [EPR25994-6] - BSA and Azide free (AB312833)

Western blot - Anti-TRIM67 antibody [EPR25994-6] - BSA and Azide free (AB312833)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise