AB150428

Anti-TRK fused gene antibody [EPR8765(2)(B)]

RabMAb
Recombinant
KO Validated
각 태그의 뜻

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Rabbit Recombinant Monoclonal TRK fused gene antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication.

10 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling TRK fused gene with ab150428 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150428 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab150428 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemical analysis of paraffin embedded Human Lung adenocarcinoma tissue using ab150428 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab150428 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunofluorescent analysis of HeLa cells labelling TRK fused gene with ab150428 at 1/250 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemical analysis of paraffin embedded Human Thyroid gland carcinoma tissue using ab150428 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

All lanes:

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 43 kDa

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Lab

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Lanes 1-4 : Merged signal (red and green). Green - ab150428 observed at 57 kDa. Red - loading control ab8245 observed at 36 kDa.

ab150428 Anti-TRK fused gene antibody [EPR8765(2)(B)] was shown to specifically react with TFG wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265841 (knockout cell lysate ab257738) was used. Wild-type and TFG knockout samples were subjected to SDS-PAGE. ab150428 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

TFG knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human TFG (TRK fused gene) knockout HeLa cell line (<a href='/ko/products/cell-lines/human-tfg-trk-fused-gene-knockout-hela-cell-line-ab265841'>ab265841</a>)

Lane 3:

HAP-1 cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 43 kDa

Observed band size: 57 kDa

false

Lab

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Lanes 1 - 4 : Merged signal (red and green). Green - ab150428 observed at 57 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab150428 was shown to react with TFG in HAP1 wild-type cells in Western blot. Loss of signal was observed when TFG knockout sample was used. HAP1 wild-type and TFG knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween^®) before incubation with ab150428 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/10000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

TFG knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

MCF7 whole cell lysate at 20 µg

Predicted band size: 43 kDa

Observed band size: 57 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8765(2)(B)

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The TRK fused gene also known as NTRK or tropomyosin receptor kinase encodes for a family of proteins integral to neurotrophin signaling. These proteins weighing approximately 80-90 kilodaltons include isoforms such as TRKA TRKB and TRKC which are products of the NTRK1 NTRK2 and NTRK3 genes respectively. They express mainly in neuronal tissues and certain non-neuronal cells. These proteins possess a diverse range of functions largely due to structural variations derived from gene fusions.

Biological function summary

Neurotrophin signaling pathways involve TRK proteins facilitating neuronal differentiation survival and plasticity. TRK proteins often form part of larger complexes engaged in transmitting signals from the extracellular environment to intracellular pathways. The TRK proteins interact with neurotrophins like NGF BDNF and NT-3 playing essential roles in nervous system function and development. The interaction between TRK receptors and their ligands activates downstream signaling cascades.

Pathways

TRK proteins significantly contribute to the MAPK/ERK and PI3K/Akt pathways. These pathways control cell growth differentiation and survival. Within these pathways TRK proteins interact with partners such as Ras and Akt coordinating a broad range of cellular processes. They ensure proper cellular responses to external stimuli and maintain homeostasis within the cell.

TRK fusion proteins frequently associate with certain cancers including thyroid and lung cancer due to gene fusions leading to overactive signaling. Altered TRK signaling correlates with neurodegenerative diseases as well such as Alzheimer's disease. TRK fusions can affect proteins like ETV6 and LMNA in the context of cancer influencing tumorigenesis and disease progression. Understanding the connections between TRK fusions and these diseases aids the development of target-specific therapies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays a role in the normal dynamic function of the endoplasmic reticulum (ER) and its associated microtubules (PubMed : 23479643, PubMed : 27813252). Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus (PubMed : 21478858).

See full target information Protein TFG

대체 명칭 보기

Protein TFG, TRK-fused gene protein, TFG

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of biomedical research 37:418-430 PubMed37977559

2023

Galectin-14 promotes hepatocellular carcinoma tumor growth enhancing heparan sulfate proteoglycan modification.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Gou,Gang Yang,Sujuan Ma,Tong Ding,Luan Sun,Fang Liu,Jin Huang,Wei Gao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRK fused gene antibody [EPR8765(2)(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (AB150428)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise