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AB264070

Anti-TrkA (pY674) + TrkB (pY706) + TrkC (pY709) antibody [EPR22298-67] - BSA and Azide free

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Rabbit Recombinant Monoclonal TrkB phospho Y706 antibody. Carrier free. Suitable for Dot, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

대체 명칭 보기

TRKB, NTRK2, BDNF/NT-3 growth factors receptor, GP145-TrkB, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Tropomyosin-related kinase B, Trk-B, MTC, TRK, TRKA, High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A, gp140trk, p140-TrkA, Trk-A, TRKC, NT-3 growth factor receptor, GP145-TrkC, Neurotrophic tyrosine kinase receptor type 3, TrkC tyrosine kinase, Trk-C

4 이미지
Western blot - Anti-TrkA (pY674) + TrkB (pY706) + TrkC (pY709) antibody [EPR22298-67] - BSA and Azide free (AB264070)
  • WB

Lab

Western blot - Anti-TrkA (pY674) + TrkB (pY706) + TrkC (pY709) antibody [EPR22298-67] - BSA and Azide free (AB264070)

This data was developed using ab229908, the same antibody clone in a different buffer formulation.

Blocking buffer and dilution concentration : 5% NFDM/TBST.

Lanes 1 - 4:

Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (<a href='/ko/products/primary-antibodies/trka-phospho-y674trkb-phospho-y706trkc-phospho-y709-antibody-epr22298-67-ab229908'>ab229908</a>) at 1/1000 dilution

Lanes 1 - 4:

Western blot - Anti-TrkA (pY674) + TrkB (pY706) + TrkC (pY709) antibody [EPR22298-67] - BSA and Azide free (ab264070) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human TrkA expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human TrkB expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human TrkC expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 140 kDa

false

Exposure time: 15s

Western blot - Anti-TrkA (pY674) + TrkB (pY706) + TrkC (pY709) antibody [EPR22298-67] - BSA and Azide free (AB264070)
  • WB

Lab

Western blot - Anti-TrkA (pY674) + TrkB (pY706) + TrkC (pY709) antibody [EPR22298-67] - BSA and Azide free (AB264070)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229908).

Blocking and diluting buffer and concentration : 5% NFDM/TBST

ab181602 was used as a GAPDH loading control at 1/200000 dilution.

All lanes:

Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (<a href='/ko/products/primary-antibodies/trka-phospho-y674trkb-phospho-y706trkc-phospho-y709-antibody-epr22298-67-ab229908'>ab229908</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human TrkA expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human TrkB expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human TrkC expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 95 kDa,140 kDa

false

Exposure time: 15s

Dot Blot - Anti-TrkA (pY674) + TrkB (pY706) + TrkC (pY709) antibody [EPR22298-67] - BSA and Azide free (AB264070)
  • Dot

Lab

Dot Blot - Anti-TrkA (pY674) + TrkB (pY706) + TrkC (pY709) antibody [EPR22298-67] - BSA and Azide free (AB264070)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229908).

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Dot Blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67] (<a href='/ko/products/primary-antibodies/trka-phospho-y674trkb-phospho-y706trkc-phospho-y709-antibody-epr22298-67-ab229908'>ab229908</a>) at 1/1000 dilution

Lane 1:

Human TrkA (phospho Y674) peptide

Lane 2:

Human TrkA (phospho Y675) peptide

Lane 3:

Human TrkA (phospho Y674/675) peptide1

Lane 4:

Human TrkA (phospho Y674/675) peptide2

Lane 5:

Human TrkA control peptide for Lane 1-4

Lane 6:

Human TrkB (phospho Y706) peptide

Lane 7:

Human TrkB (phospho Y707) peptide

Lane 8:

Human TrkB (phospho Y706/707) peptide

Lane 9:

Human TrkB control peptide for Lane 6-8

Lane 10:

Human TrkC (phospho Y709) peptide

Lane 11:

Human TrkC (phospho Y710) peptide

Lane 12:

Human TrkC (phospho Y709/710) peptide

Lane 13:

Human TrkC control peptide for Lane 10-12

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Dot Blot - Anti-TrkA (pY674) + TrkB (pY706) + TrkC (pY709) antibody [EPR22298-67] - BSA and Azide free (AB264070)
  • Dot

Unknown

Dot Blot - Anti-TrkA (pY674) + TrkB (pY706) + TrkC (pY709) antibody [EPR22298-67] - BSA and Azide free (AB264070)

Dot blot analysis using ab229908 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

Lane 1 : TrkB (phospho T705) peptide (aa697-708)

Lane 2 : TrkB (phospho T705) peptide (aa702-712)

Lane 3 : TrkB non-phospho peptide (aa697-712)

Blocking/diluting buffer and concentration : 5% NFDM/TBST This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229908).

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Unconjugated

    Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR22298-67]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22298-67

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>The antibody shows low affinity in the natural tissues of mouse and rat</p>

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대체품

Primary Antibodies

AB197072

Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR19399]

RabMAb|Recombinant

Western blot - Anti-TrkA (phospho Y674)+TrkB (phospho Y706)+TrkC (phospho Y709) antibody [EPR19399] (AB197072)

  • 1
  • 1
Primary Antibodies

AB134155

Anti-TrkB antibody [EPR1294]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] (AB134155)

  • 5
  • 1
Primary Antibodies

AB223155

Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-TrkB antibody [EPR17805-146] - Low endotoxin, Azide free (AB223155)

  • 0
  • 0
Primary Antibodies

AB248704

Anti-TrkB antibody [EPR1294] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TrkB antibody [EPR1294] - BSA and Azide free (AB248704)

  • 0
  • 0
Primary Antibodies

AB187041

Anti-TrkB antibody [EPR17805-146]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-TrkB antibody [EPR17805-146] (AB187041)

  • 0
  • 1

보완 제품

Primary Antibodies

AB108319

Anti-BDNF antibody [EPR1292]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Western blot - Anti-BDNF antibody [EPR1292] (AB108319)

  • 4
  • 10
Primary Antibodies

AB187041

Anti-TrkB antibody [EPR17805-146]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-TrkB antibody [EPR17805-146] (AB187041)

  • 0
  • 1
Primary Antibodies

AB32515

Anti-CREB antibody [E306]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB antibody [E306] (AB32515)

  • 5
  • 4
Primary Antibodies

AB32503

Anti-Bax antibody [E63]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (AB32503)

  • 4
  • 12
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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제품 세부 정보

ab264070 is the carrier-free version of ab229908.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TrkB also known as Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) is a cell surface receptor with a mass of approximately 145 kDa. It binds brain-derived neurotrophic factor (BDNF) and is widely expressed in the central nervous system particularly in areas like the hippocampus cortex and cerebellum. TrkB belongs to the family of receptor tyrosine kinases functioning by catalyzing the transfer of a phosphate group from ATP to specific tyrosine residues on substrate proteins in the receptor triggering downstream signaling cascades.
Biological function summary

TrkB plays essential roles in neuronal survival differentiation and synaptic plasticity. It acts as an important mediator for the effects of neurotrophins primarily BDNF on neurons. While TrkB itself is not part of a multi-protein complex it interacts closely with several adaptor proteins post-activation to propagate survival and proliferation signals within the neuron. These biochemical interactions greatly influence neurodevelopment and cognitive functions.

Pathways

TrkB is a pivotal player in both the MAPK/ERK and PI3K/AKT pathways. Upon BDNF binding TrkB undergoes autophosphorylation triggering these pathways which are critically involved in cell survival and growth. Other proteins such as SHC1 and PLCγ interact with TrkB in these pathways extending the range of cellular responses to neurotrophin signaling facilitating processes like neuronal plasticity and memory formation.

TrkB has significant implications in neurological conditions like Alzheimer's disease and depression. In Alzheimer's disease alterations in TrkB signaling can result in impaired cognitive functions and neuronal loss where it interacts with proteins like amyloid-beta contributing to disease progression. In depression dysfunctional BDNF-TrkB signaling impacts mood regulation and resilience linking TrkB with alterations in serotonin receptor signaling emphasizing its role in mood disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity (By similarity). Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2 (PubMed : 15494731, PubMed : 7574684). Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation (PubMed : 15494731). Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.
See full target information NTRK2 pY706

Additional targets

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 25: PubMed38542462

2024

Differential Expression of CKLF-like MARVEL Transmembrane Domain-Containing Protein 6 and Programmed Cell Death Ligand 1 as Prognostic Biomarkers in Upper Tract Urothelial Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Said Kdimati,Clemens Christoph,Änne Glass,Nadja Engel,Desiree-Louise Dräger,Claudia Maletzki,Anne-Sophie Becker,Annette Zimpfer
제품이 사용된 논문 모두 보기
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Product promise

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