AB157215

Anti-Tropomodulin 3 antibody [EPR10303(B)]

RabMAb
Recombinant
각 태그의 뜻

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(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Tropomodulin 3 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomodulin 3 antibody [EPR10303(B)] (AB157215)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomodulin 3 antibody [EPR10303(B)] (AB157215)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling Tropomodulin 3 with ab157215 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomodulin 3 antibody [EPR10303(B)] (AB157215)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Tropomodulin 3 with ab157215 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Tropomodulin 3 antibody [EPR10303(B)] (AB157215)

All lanes:

Western blot - Anti-Tropomodulin 3 antibody [EPR10303(B)] (ab157215) at 1/1000 dilution

All lanes:

Immunoprecipitation pellet from Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg

Predicted band size: 40 kDa,65 kDa

false

Unknown

Western blot - Anti-Tropomodulin 3 antibody [EPR10303(B)] (AB157215)

All lanes:

Western blot - Anti-Tropomodulin 3 antibody [EPR10303(B)] (ab157215) at 1/1000 dilution

Lane 1:

C2C12 cell lysate at 10 µg

Lane 2:

C6 cell lysate at 10 µg

Lane 3:

RAW 264.7 cell lysate at 10 µg

Lane 4:

Caco-2 cell lysate at 10 µg

Predicted band size: 40 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10303(B)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 79% identity (92% positives) with Tropomodulin-2. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Tropomodulin 3 also known as TMOD3 serves an important role in regulating the dynamics of the actin cytoskeleton. It caps the pointed end of actin filaments which prevents both their elongation and disassembly. TMOD3 has a molecular mass of approximately 40 kDa. This protein is expressed in various tissues including the heart liver and lungs indicating its broad functional significance across multiple systems.

Biological function summary

Tropomodulin 3 interacts with components of the cytoskeleton to help maintain cell shape and stability. It often functions as part of a multi-protein complex with tropomyosin which stabilizes the actin filament and protects it from depolymerization. Through its interaction with actin and tropomyosin TMOD3 influences cell motility division and muscle contraction essential processes in cellular and tissue dynamics.

Pathways

Tropomodulin 3 plays a part in the actin cytoskeleton regulation pathway. It regulates the turnover and length of actin filaments which connect to processes like cell adhesion and movement. The regulation of actin filament dynamics by TMOD3 influences interactions with associated proteins such as myosin which drives cellular movements and mechanical support.

Tropomodulin 3 has been linked to specific pathologies such as cardiomyopathies and certain cancers. In conditions of cardiomyopathy TMOD3 expression or function disturbances can lead to improper cardiac muscle function often involving related proteins like myosin and tropomyosin. Moreover aberrant TMOD3 activity has also been associated with cancer metastasis where altered actin dynamics contribute to increased cell invasiveness and tumor spread.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Blocks the elongation and depolymerization of the actin filaments at the pointed end. The Tmod/TM complex contributes to the formation of the short actin protofilament, which in turn defines the geometry of the membrane skeleton (By similarity).

See full target information TMOD3

대체 명칭 보기

Tropomodulin-3, Ubiquitous tropomodulin, U-Tmod, TMOD3

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of cellular and molecular medicine 27:2437-2447 PubMed37436074

2023

PSMC4 promotes prostate carcinoma progression by regulating the CBX3-EGFR-PI3K-AKT-mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kaifeng Liu,Shengmin Zhang,Yongzhan Gong,Panyan Zhu,Weigan Shen,Qi Zhang

The Journal of clinical investigation 131: PubMed33586680

2021

Aberrant AZIN2 and polyamine metabolism precipitates tau neuropathology.

Applications

Unspecified application

Species

Unspecified reactive species

Leslie A Sandusky-Beltran,Andrii Kovalenko,Devon S Placides,Kevin Ratnasamy,Chao Ma,Jerry B Hunt,Huimin Liang,John Ivan T Calahatian,Camilla Michalski,Margaret Fahnestock,Laura J Blair,April L Darling,Jeremy D Baker,Sarah N Fontaine,Chad A Dickey,Joshua J Gamsby,Kevin R Nash,Erin Abner,Maj-Linda B Selenica,Daniel C Lee

Molecular carcinogenesis 58:1897-1907 PubMed31313392

2019

Tropomodulin 3 modulates EGFR-PI3K-AKT signaling to drive hepatocellular carcinoma metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zheng,Yuan Yang,Yong-Gang Hong,Meng-Chao Wang,Sheng-Xian Yuan,Zhen-Guang Wang,Feng-Rui Bi,Li-Qiang Hao,Hong-Li Yan,Wei-Ping Zhou

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tropomodulin 3 antibody [EPR10303(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomodulin 3 antibody [EPR10303(B)] (AB157215)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tropomodulin 3 antibody [EPR10303(B)] (AB157215)

Western blot - Anti-Tropomodulin 3 antibody [EPR10303(B)] (AB157215)

Western blot - Anti-Tropomodulin 3 antibody [EPR10303(B)] (AB157215)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise