AB7785

Anti-Tropomyosin antibody [TM-311]

(1 Review)

(17 Publications)

Mouse Monoclonal TPM1 antibody. Suitable for WB and reacts with Human samples. Cited in 17 publications.

대체 명칭 보기

Tropomyosin alpha-1 chain, Alpha-tropomyosin, Tropomyosin-1, TPM1

1 Images

Unknown

Western blot - Anti-Tropomyosin antibody [TM-311] (AB7785)

All lanes:

Western blot - Anti-Tropomyosin antibody [TM-311] (ab7785) at 1/200 dilution

All lanes:

Human heart tissue lysate - total protein (ab29431) at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 33 kDa

Observed band size: 37 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

TM-311

Isotype

IgG1

Light chain 타입

kappa

Carrier free

Reacts with

Human

Applications

applications

Specificity

This antibody is specific for the 36K and 39K bands (alpha isoform at 39 kDa and beta isoform at 36 kDa )of chicken gizzard tropomyosin. It is not specific for the alpha form alone. This antibody stains human fibroblast cells, and it cross-reacts with chicken fibroblast and L8 rat muscle cells.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/200", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

보관 버퍼

Preservative: 0.1% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Tropomyosin also known as anti-TM is a coiled-coil protein with a mass typically around 32 to 37 kDa depending on the isoform. This protein exists in both muscle and non-muscle cells playing essential roles in stabilizing actin filaments. In muscle tissues tropomyosin is part of the thin filament structure. It is found in high concentrations in skeletal cardiac and smooth muscles and is responsible for regulating the interaction between actin and myosin which is important for muscle contraction.

Biological function summary

In muscle contraction and cellular movement tropomyosin acts as a regulatory protein. It associates with the troponin complex to control the binding of myosin heads to actin filaments. By shifting its position on the actin filament tropomyosin enables the muscle contraction cycle to proceed. It is an integral part of the thin filament complex interacting with actin and troponin and is essential for the calcium-dependent regulation of muscle contraction.

Pathways

Tropomyosin plays a significant role in the actin cytoskeleton regulation pathway. It influences the dynamics of actin filament stability and organization which are important for cell motility and structure. The protein also interacts with the calmodulin-dependent protein kinase II (CaMKII) pathway where its interaction influences heart and skeletal muscle functionality. In these pathways tropomyosin regulates the activities of proteins such as myosin and actin ensuring proper cellular mechanical function and signaling.

Alterations in tropomyosin function can lead to several muscle-related conditions. Hypertrophic cardiomyopathy and dilated cardiomyopathy are two disorders associated with mutations in tropomyosin. In these conditions improper regulation of the actin-myosin interaction disrupts normal cardiac muscle function. Tropomyosin-related disorders often involve interactions with proteins such as troponin which is another key player in the regulation of muscle contraction and is important for maintaining a healthy cardiac rhythm.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.

See full target information TPM1

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제품이 사용된 논문 (17)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of medical virology 96:e29379 PubMed38235617

2024

Vertical mother-to-infant transmission of herpes simplex virus 2 is correlated with tropism due to mutations in viral UL13.

Applications

Unspecified application

Species

Unspecified reactive species

Kimiyasu Shiraki,Tohru Daikoku,Mark N Prichard,Koma Matsuo,Tomoko Okuda,Yoshihiro Yoshida,Masaya Takemoto,Kenji Takeuchi,Kiyonao Sada,Richard Whitley,Takashi Kawana

Life (Basel, Switzerland) 12: PubMed36013400

2022

Effects of Obesity and Diabesity on Ventricular Muscle Structure and Function in the Zucker Rat.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Sultan,Ernest Adeghate,Bright Starling Emerald,Muhammad A Qureshi,Saeed Tariq Minhas,Frank Christopher Howarth

Neoplasma 66:203-210 PubMed30509103

2018

Skullcapflavone I inhibits proliferation of human colorectal cancer cells via down-regulation of miR-107 expression.

Applications

Unspecified application

Species

Unspecified reactive species

W Zhang,W Li,X Han

Mechanisms of ageing and development 171:24-30 PubMed29510160

2018

Tropomyosin 2 heterozygous knockout in mice using CRISPR-Cas9 system displays the inhibition of injury-induced epithelial-mesenchymal transition, and lens opacity.

Applications

IHC-P

Species

Mouse

Teppei Shibata,Shinsuke Shibata,Yasuhito Ishigaki,Etsuko Kiyokawa,Masahito Ikawa,Dhirendra P Singh,Hiroshi Sasaki,Eri Kubo

Molecular biology of the cell 28:2531-2542 PubMed28720661

2017

Stabilization of F-actin by tropomyosin isoforms regulates the morphology and mechanical behavior of red blood cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhua Sui,David S Gokhin,Roberta B Nowak,Xinhua Guo,Xiuli An,Velia M Fowler

Molecular biology of the cell 28:535-544 PubMed27974641

2016

Proper cytoskeletal architecture beneath the plasma membrane of red blood cells requires .

Applications

Unspecified application

Species

Unspecified reactive species

Faryal Ijaz,Yasue Hatanaka,Takahiro Hatanaka,Koji Tsutsumi,Takayuki Iwaki,Kazuo Umemura,Koji Ikegami,Mitsutoshi Setou

Scientific reports 5:11358 PubMed26079396

2015

Visualizing the appearance and disappearance of the attractor of differentiation using Raman spectral imaging.

Applications

Species

Mouse

Taro Ichimura,Liang-da Chiu,Katsumasa Fujita,Hiroaki Machiyama,Satoshi Kawata,Tomonobu M Watanabe,Hideaki Fujita

Scientific reports 4:4781 PubMed24759171

2014

Induction of functional tissue-engineered skeletal muscle constructs by defined electrical stimulation.

Applications

Species

Mouse

Akira Ito,Yasunori Yamamoto,Masanori Sato,Kazushi Ikeda,Masahiro Yamamoto,Hideaki Fujita,Eiji Nagamori,Yoshinori Kawabe,Masamichi Kamihira

The Journal of biological chemistry 289:11616-29 PubMed24644292

2014

Differential actin-regulatory activities of Tropomodulin1 and Tropomodulin3 with diverse tropomyosin and actin isoforms.

Applications

Species

Rabbit

Sawako Yamashiro,David S Gokhin,Zhenhua Sui,Sarah E Bergeron,Peter A Rubenstein,Velia M Fowler

Journal of proteomics 75:5386-95 PubMed22749930

2012

Effect of the myosin light chain kinase inhibitor ML-7 on the proteome of hearts subjected to ischemia-reperfusion injury.

Applications

Species

Rat

Han-bin Lin,Virgilio J J Cadete,Jolanta Sawicka,Mieczyslaw Wozniak,Grzegorz Sawicki

제품이 사용된 논문 모두 보기

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Product promise

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Anti-Tropomyosin antibody [TM-311]

Western blot - Anti-Tropomyosin antibody [TM-311] (AB7785)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Specificity

대체품

Primary Antibodies

보완 제품

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (17)

Product promise